A5053296205- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Retinal Development and Disorders
- Hormonal Regulation and Hypertension
- Neuroscience and Neuropharmacology Research
- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Circadian rhythm and melatonin
- Dietary Effects on Health
- FOXO transcription factor regulation
- Receptor Mechanisms and Signaling
- Photochromic and Fluorescence Chemistry
- Cancer Research and Treatments
- Genomics, phytochemicals, and oxidative stress
- Photoreceptor and optogenetics research
- Cancer-related Molecular Pathways
- Heart Failure Treatment and Management
- Nutrition, Genetics, and Disease
- Cardiomyopathy and Myosin Studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Pancreatic function and diabetes
- Adrenal Hormones and Disorders
- Cardiovascular Health and Risk Factors
- Retinal and Macular Surgery
Cincinnati Children's Hospital Medical Center
2021-2024
University of Cincinnati Medical Center
2021-2024
University of Cincinnati
2023-2024
University of Michigan
2024
Oncology Hematology Care
2022
Michigan United
2021
W.K. Kellogg Foundation
2021
Sarcopenia burdens the elderly population through loss of muscle energy and mass, yet treatments to functionally rescue both parameters are missing. The glucocorticoid prednisone remodels metabolism based on frequency intake, but its mechanisms in sarcopenia unknown. We found that once-weekly intermittent rescued quality aged 24-month-old mice levels comparable young 4-month-old mice. discovered an age- sex-independent receptor transactivation program encompassing PGC1α co-factor Lipin1....
Circadian time-of-intake gates the cardioprotective effects of glucocorticoid administration in both healthy and infarcted hearts. The cardiomyocyte-specific receptor (GR) its co-factor, Krüppel-like factor (Klf15), play critical roles maintaining normal heart function long-term serve as pleiotropic regulators cardiac metabolism. Despite this understanding, cardiomyocyte-autonomous metabolic targets influenced by concerted epigenetic action GR-Klf15 axis remain undefined. Here, we...
Photoreceptor loss results in vision many blinding diseases, and metabolic dysfunction underlies photoreceptor degeneration. So, exploiting metabolism is an attractive strategy to prevent loss. Yet, the pathways that maintain health remain largely unknown. Here, we investigated dependence of photoreceptors on glutamine (Gln) catabolism. Gln converted glutamate via glutaminase (GLS), so mice lacking GLS rod were generated inhibit Loss produced rapid In vivo metabolomic methodologies...
Mitochondrial capacity is critical to adapt the high energy demand of heart circadian oscillations and diseased states. Glucocorticoids regulate cycle metabolism, but little known about how timing exogenous glucocorticoid dosing directly regulates metabolism through cardiomyocyte-autonomous mechanisms. While chronic once-daily intake glucocorticoids promotes metabolic stress failure, we recently discovered that intermittent once-weekly promoted muscle in normal obese skeletal muscle....
Skeletal muscle disorders commonly affect the function and integrity of muscles. Novel interventions bring new potential to rescue or alleviate symptoms associated with these disorders. In vivo in vitro testing mouse models allows quantitative evaluation degree dysfunction, therefore, level rescue/restoration by target intervention. Several resources methods are available assess lean mass, as well myofiber typing separate concepts; however, a technical resource unifying is missing. Here, we...
Photoreceptor loss results in vision many blinding diseases, and metabolic dysfunction underlies photoreceptor degeneration. So, exploiting metabolism is an attractive strategy to prevent loss. Yet, the pathways that maintain health remain largely unknown. Here, we investigated dependence of photoreceptors on Gln catabolism. converted glutamate via glutaminase (GLS), so mice lacking GLS rod were generated inhibit Loss produced rapid In vivo metabolomic methodologies supplementation...
Photoreceptor loss results in vision many blinding diseases, and metabolic dysfunction underlies photoreceptor degeneration. So, exploiting metabolism is an attractive strategy to prevent loss. Yet, the pathways that maintain health remain largely unknown. Here, we investigated dependence of photoreceptors on Gln catabolism. converted glutamate via glutaminase (GLS), so mice lacking GLS rod were generated inhibit Loss produced rapid In vivo metabolomic methodologies supplementation...
Genetic variations in the glucocorticoid receptor (GR) gene NR3C1 can impact metabolism. The single nucleotide polymorphism (SNP) rs6190 (p.R23K) has been associated humans with enhanced metabolic health, but SNP mechanism of action remains completely unknown. We generated a transgenic knock-in mice genocopying this to elucidate how mutant GR impacts Compared non-mutant littermates, showed increased muscle insulin sensitivity and strength on regular chow high-fat diet, blunting diet-induced...
The development and maintenance of the neuromuscular junction (NMJ) requires reciprocal signals between nerve terminals multinucleated skeletal muscle fiber (myofiber). This interaction leads to highly specialized transcription in sub-synaptic or NMJ myonuclei within mature myofibers leading clustering acetylcholine receptors (AChRs). Here, we utilized single-nucleus RNA sequencing (snRNA-seq) delineate transcriptional response denervation. Through snRNA-seq on from two independent mouse...
Elevated cholesterol poses a significant cardiovascular risk, particularly in older women. The glucocorticoid receptor (GR), crucial nuclear transcription factor that regulates the metabolism of virtually all major nutrients, harbors still undefined role regulation. Here, we report coding single nucleotide polymorphism (SNP) gene encoding GR,
Photoreceptor loss results in vision many blinding diseases, and metabolic dysfunction underlies photoreceptor degeneration. So, exploiting metabolism is an attractive strategy to prevent loss. Yet, the pathways that maintain health remain largely unknown. Here, we investigated dependence of photoreceptors on Gln catabolism. converted glutamate via glutaminase (GLS), so mice lacking GLS rod were generated inhibit Loss produced rapid In vivo metabolomic methodologies supplementation...
Abstract Sarcopenia burdens the elderly population through loss of muscle energy and mass, yet treatments to functionally rescue both parameters are missing. The glucocorticoid prednisone remodels metabolism based on frequency intake, but its mechanisms in sarcopenia unknown. We found that once-weekly intermittent rescued quality aged 24-month-old mice levels comparable young 4-month-old mice. discovered an age- sex-independent receptor transactivation program encompassing PGC1alpha...
Abstract Disclosure: A. Prabakaran: None. M. Wintzinger: K. Piczer: Miz: Walton: H. Durumutla: Quattrocelli: Glucocorticoids (GCs) are circadian hormones regulating metabolism by activating the glucocorticoid receptor (GR) as pleiotropic transcription factor. Timing of exogenous GC drug regimens is emerging a key determinant their metabolic effects. This was shown surprising finding that once-weekly intermittence reverses many pro-obesogenic side effects once-daily dosing in mice and humans....
Circadian time-of-intake gates the cardioprotective effects of glucocorticoid administration in both healthy and infarcted hearts. The cardiomyocyte-specific receptor (GR) its co-factor, Krüppel-like factor (Klf15), play critical roles maintaining normal heart function long-term serve as pleiotropic regulators cardiac metabolism. Despite this understanding, cardiomyocyte-autonomous metabolic targets influenced by concerted epigenetic action GR-Klf15 axis remain undefined. Here, we...
Adiponectin is the most abundant adipokine in our circulation and regulates inter-tissue communication like adipose-muscle axis. Pharmacological modulation of this crosstalk beneficial promoting exercise tolerance metabolic stress conditions obesity. Endogenous glucocorticoids regulate function response, but chronic daily intake exogenous produces or exacerbates stress. However, stark contrast to intake, we discovered that intermittent regimens glucocorticoids, e.g. once-weekly, improves...
Glucocorticoids are circadian regulators of energy balance. However, the cardiomyocyte-autonomous mechanisms glucocorticoid action on heart metabolism remain unresolved. Also, little attention is given to impact time intake effects these drugs in heart. Here, we investigated whether exposure gates synthetic glucocorticoids and function. In single pulse experiments, compared prednisone wildtype (WT) hearts through light-phase- versus dark-phase-specific injections. Light-phase - but not...
Abstract Mitochondrial capacity is critical to adapt the high energy demand of heart circadian oscillations and diseased states. Glucocorticoids regulate cycle metabolism, but little known about how timing exogenous glucocorticoid dosing directly regulates metabolism through cardiomyocyte-autonomous mechanisms. While chronic oncedaily intake glucocorticoids promotes metabolic stress failure, we recently discovered that intermittent once-weekly promoted muscle in normal obese skeletal muscle....