A5054097304- RNA Research and Splicing
- Reproductive Biology and Fertility
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Microtubule and mitosis dynamics
- Renal and related cancers
- Historical Linguistics and Language Studies
- Phagocytosis and Immune Regulation
- French Language Learning Methods
- DNA Repair Mechanisms
- Linguistics and Discourse Analysis
- Marine Invertebrate Physiology and Ecology
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Marine Sponges and Natural Products
- Genetics and Neurodevelopmental Disorders
- Prenatal Screening and Diagnostics
- RNA and protein synthesis mechanisms
- PI3K/AKT/mTOR signaling in cancer
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2021-2025
Charles University
2023
Université Grenoble Alpes
2019
Examining early-branching animal phyla can help reconstructing the evolutionary origin of immune cells. Here we characterized related cell program in sea anemone Nematostella vectensis, a model organism representing phylum Cnidaria that diverged ~600 million years ago from rest animals. By using transgenic reporter line expressing mCherry under RLRb antiviral promoter show cells fluorescent upon stimulation with viral mimic poly(I:C) are morphologically and transcriptomically distinct. These...
Translation is critical for development as transcription in the oocyte and early embryo silenced. To illustrate translational changes during meiosis consecutive two mitoses of embryo, we performed a genome-wide translatome analysis. Acquired data showed significant uniform activation key initiation elongation axes specific to M-phases. Although global protein synthesis decreases M-phases, translation activity increases uniformly fluctuating manner, leading qualitative regulation via...
Mammalian oocyte development depends on the temporally controlled translation of maternal transcripts, particularly in coordination meiotic and early embryonic when transcription has ceased. The mRNA is regulated by various RNA-binding proteins. We show that absence cytoplasmic polyadenylation element-binding protein 3 (CPEB3) negatively affects female reproductive fitness. CPEB3-depleted oocytes undergo meiosis normally but experience arrest due to a disrupted transcriptome, leading...
In mammalian females, oocytes are stored in the ovary and meiosis is arrested at diplotene stage of prophase I. When females reach puberty selectively recruited cycles to grow, overcome meiotic arrest, complete first division become mature (ready for fertilization). At a molecular level, master regulator I arrest resumption maturation-promoting factor (MPF) complex, formed by active form cyclin dependent kinase 1 (CDK1) Cyclin B1. However, we still do not have information regarding factors...
Regulation of translation is essential for the diverse biological processes involved in development. Particularly, mammalian oocyte development requires precisely controlled maternal transcripts to coordinate meiotic and early embryo progression while transcription silent. It has been recently reported that key components mRNA control are short long noncoding RNAs (ncRNAs). We found ncRNABrain cytoplasmic 1 (BC1) a role fully grown germinal vesicle (GV) mouse oocyte, where highly expressed...
Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A part stored belongs to long non-coding class which contain either transcriptional noise or important contributors cellular physiology. Despite expanding number studies related lncRNAs, their influence on oocyte physiology remains enigmatic. We found an specific antisense, RNA, "Rose" (lncRNA in Oocyte Specifically Expressed) expressed two variants containing and three...
Abstract Transcriptional activity and gene expression are essential for the development of a mature, meiotically competent oocyte. We have found that absence cyclin-dependent kinase 12 (CDK12) in oocytes leads to complete female sterility, as there no fully developed able accomplish meiosis I ovaries. Mechanistically, CDK12 growing controls POLII maintenance physiological maternal transcriptome, which negatively affects protein synthesis promotes further oocyte growth. In addition,...