A5021113194- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Neuroscience and Neural Engineering
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Functional Brain Connectivity Studies
- Neurotransmitter Receptor Influence on Behavior
- Tryptophan and brain disorders
- EEG and Brain-Computer Interfaces
- Stress Responses and Cortisol
- Neural Networks and Applications
- stochastic dynamics and bifurcation
- Immune cells in cancer
- Schizophrenia research and treatment
- Neurogenesis and neuroplasticity mechanisms
- Pain Mechanisms and Treatments
- Photoreceptor and optogenetics research
- Anesthesia and Neurotoxicity Research
- Cholinesterase and Neurodegenerative Diseases
- Vestibular and auditory disorders
- Nicotinic Acetylcholine Receptors Study
- Cardiac electrophysiology and arrhythmias
- Single-cell and spatial transcriptomics
University of Pittsburgh
2015-2025
Lewis University
2024
Pittsburg State University
2015
Massachusetts Institute of Technology
2014
Center for the Neural Basis of Cognition
2000-2002
University of Buenos Aires
1991-1997
Reactive oxygen species, including superoxide, generally are considered neurotoxic molecules whose effects can be alleviated by antioxidants. Different from this view, we show that scavenging of superoxide with an antioxidant enzyme is associated deficits in hippocampal long-term potentiation (LTP), a putative neural substrate memory, and hippocampal-mediated memory function. Using transgenic mice overexpress extracellular dismutase (EC-SOD), scavenger, found LTP was impaired area CA1...
In the primate neocortex, little is known about possible associations between functional subclasses of GABA neurons, their morphological properties and calcium-binding protein (CaBP) content. We used whole-cell current clamp recordings, combined with intracellular labeling fluorescence immunohistochemistry, to determine these relationships for interneurons in layers 2–3 monkey prefrontal cortex (PFC). Eighty-one were included analysis. Thirty-eight cells showed immunoreactivity one three...
Schizophrenia may involve hypofunction of NMDA receptor (NMDAR)-mediated signaling, and alterations in parvalbumin-positive fast-spiking (FS) GABA neurons that cause abnormal gamma oscillations. It was recently hypothesized prefrontal cortex (PFC) FS neuron activity is highly dependent on NMDAR activation that, consequently, dysfunction schizophrenia secondary to hypofunction. However, NMDARs are abundant synapses onto PFC pyramidal neurons; thus, a key question whether or cell more NMDARs....
Parvalbumin-positive (PV+) neurons control the timing of pyramidal cell output in cortical neuron networks. In prefrontal cortex (PFC), PV+ activity is involved cognitive function, suggesting that maturation critical for development. The two major subtypes found PFC, chandelier cells (ChCs) and basket (BCs), are thought to play different roles circuits, but trajectories their physiological have not been compared. Using separate mouse lines, we mature both ChCs BCs abundant superficial layer...
Importance The risk of mental disorders is consistently associated with variants in CACNA1C (L-type calcium channel Cav1.2) but it not known why these channels are critical to cognition, and whether they affect the layer III pyramidal cells dorsolateral prefrontal cortex that especially vulnerable cognitive disorders. Objective To examine molecular mechanisms expressed primate cortices. Design, Setting, Participants design included transcriptomic analyses from human macaque cortex,...
Dopaminergic modulation of neuronal networks in the dorsolateral prefrontal cortex (PFC) is believed to play an important role information processing during working memory tasks both humans and nonhuman primates. To understand basic cellular mechanisms that underlie these actions dopamine (DA), we have investigated influence DA on properties layer 3 pyramidal cells area 46 macaque monkey PFC. Intracellular voltage recordings were obtained with sharp whole cell patch-clamp electrodes a PFC...
Recent studies suggest that fast-spiking (FS) interneurons of the monkey dorsolateral prefrontal cortex (DLPFC) exhibit task-related firing during working-memory tasks. To gain further understanding functional role FS neurons in DLPFC, we described vitro electrophysiological properties and their synaptic connections with pyramidal cells layers 2/3 areas 9 46. Extracellular spike duration was found to distinguish from non-FS interneuron subtypes. However, a substantial overlap extracellular...
In monkey dorsolateral prefrontal cortex (PFC), long-distance, horizontally oriented intrinsic axon collaterals interconnect clusters of pyramidal neurons in the supragranular layers. order to study electrophysiological responses mediated by these long-distance projections, an vitro slice preparation PFC was used obtain whole-cell patch clamp recordings from layer 3 neurons. Using vivo tracer injections, we found that projections were well preserved slices cut coronal plane. Postsynaptic...
In the prefrontal cortex (PFC) during working memory tasks fast-spiking (FS) interneurons might shape spatial selectivity of pyramidal cell firing. order to provide time control activity, incoming excitatory inputs should excite FS more vigorously than cells. This can be achieved if subthreshold responses are considerably stronger and faster those in neurons. Here we compared functional properties post-synaptic potentials (EPSPs) between cells slices from monkey dorsolateral PFC rat...
In primates, little is known about intrinsic electrophysiological properties of neocortical neurons and their morphological correlates. To classify inhibitory cells (interneurons) in layers 2-3 monkey dorsolateral prefrontal cortex we used whole cell voltage recordings intracellular labeling slice preparation with subsequent reconstructions. Regular spiking pyramidal have been also included the sample. Neurons were successfully segregated into three physiological clusters: regular-,...
The heterogeneity of γ-aminobutyric acid interneurons in the rodent neocortex is well-established, but their classification into distinct subtypes remains a matter debate. primate further complicated by less extensive database features these neurons and reported interspecies differences. Consequently, this study we characterized 8 different morphological types from monkey prefrontal cortex, 4 which have not been previously classified. These interneuron differed expression molecular markers...
Differences in the developmental origin and relative proportions of biochemically distinct classes cortical neurons have been found between rodents primates. In addition, species differences properties certain cell types, such as neurogliaform cells, also reported. Consequently, this study we compared anatomical physiological parvalbumin (PV)-positive basket interneurons prefrontal cortex macaque monkeys rats. The somal size, total dendritic length, horizontal vertical spans axonal arbor...
Depolarization-induced suppression of inhibition (DSI) is a prevailing form endocannabinoid signalling. However, several discrepancies have arisen regarding the roles played by two major brain endocannabinoids, 2-arachidonoylglycerol (2-AG) and anandamide, in mediating DSI. Here we studied signalling prefrontal cortex (PFC), where components system been identified, but remains largely unexplored. In voltage clamp recordings from mouse PFC pyramidal neurons, depolarizing steps significantly...