A5037484413- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Cytomegalovirus and herpesvirus research
- Tryptophan and brain disorders
- Neurotransmitter Receptor Influence on Behavior
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Herpesvirus Infections and Treatments
- Cellular transport and secretion
- Stress Responses and Cortisol
- Ion channel regulation and function
- Toxoplasma gondii Research Studies
- Cannabis and Cannabinoid Research
- Neurogenesis and neuroplasticity mechanisms
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- GABA and Rice Research
- Immune Cell Function and Interaction
- Functional Brain Connectivity Studies
- HIV Research and Treatment
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Adipose Tissue and Metabolism
University of Pittsburgh
2016-2025
University of Pittsburgh Medical Center
2022-2025
Center for the Neural Basis of Cognition
2023
Carnegie Mellon University
2023
VA Pittsburgh Healthcare System
2013-2017
Northwestern University
2017
Translational Research in Oncology
2015
Center for Neurosciences
2015
Mental Illness Research, Education and Clinical Centers
2013
Library and Archives Canada
2008
The human cytomegalovirus (HCMV) early glycoprotein products of the US11 and US2 open reading frames cause increased turnover major histocompatibility complex (MHC) class I heavy chains. Since is homologous to another HCMV gene (US3), we hypothesized that US3 product also may affect MHC expression. In cells constitutively expressing gene, chains formed a stable with beta 2-microglobulin. However, maturation N-linked glycan was impaired in US3+ cells. (gpUS3) occurs mostly high-mannose form...
Cognitive deficits in schizophrenia are associated with altered activity of the dorsolateral prefrontal cortex, which has been attributed to lower expression 67 kDa isoform glutamic acid decarboxylase (GAD67), major γ-aminobutyric (GABA)-synthesizing enzyme. However, little is known about relationship GAD67 mRNA levels and illness severity, translation transcript into protein, protein axon terminals, key site GABA production function.Quantitative polymerase chain reaction was used measure...
Cargo selection and export from the endoplasmic reticulum is mediated by COPII coat machinery that includes small GTPase Sar1 Sec23/24 Sec13/31 complexes. We have analyzed sequential events regulated purified complexes during synchronized of cargo ER in vitro. find activation alone, absence other cytosolic components, leads to formation ER-derived tubular domains resemble transitional elements initiate selection. These Sar1-generated were shown be transient, functional intermediates Golgi...
ABSTRACT Human herpesvirus 8 (HHV8) infects Kaposi’s sarcoma (KS) spindle cells in situ, as well the lesional endothelial considered to be cell precursors. The HHV8 genome contains several oncogenes, suggesting that infection of and could induce cellular transformation tumorigenesis promote formation KS lesions. To investigate potential contribute development KS, we have developed an vitro model utilizing dermal microvascular support significant infection. In contrast existing systems used...
ABSTRACT We have previously demonstrated reactivation of latent human cytomegalovirus (HCMV) in myeloid lineage cells obtained from healthy donors. Virus was allogenically stimulated monocyte-derived macrophages (Allo-MDM), but not differentiated by mitogenic stimulation (ConA-MDM). In the present study, cellular and cytokine components essential for HCMV replication were examined Allo-MDM. The importance both CD4 + CD8 T generation HCMV-permissive Allo-MDM negative selection or blocking...
Deficient excitatory drive to parvalbumin-containing cortical interneurons is proposed as a key neural substrate for altered gamma oscillations and cognitive dysfunction in schizophrenia. However, pathological entity producing such deficit has not been identified. The authors tested the hypothesis that parvalbumin receive fewer synaptic inputs individuals with schizophrenia.Fluorescent immunohistochemistry, confocal microscopy, post-image processing techniques were used quantify number of...
Objective: Decreased density of dendritic spines in adult schizophrenia subjects has been hypothesized to result from increased pruning excess synapses adolescence. In vivo imaging studies have confirmed that synaptic is largely driven by the loss large or mature synapses. Thus, throughout adolescence would likely a deficit adulthood. Here, authors examined and volume deep layer 3 auditory cortex 20 matched comparison as well aberrant voltage-gated calcium channel subunit protein expression...
Objective: In schizophrenia, somatostatin (SST) and parvalbumin (PV) mRNA levels are lower in the dorsolateral prefrontal cortex (DLPFC), but it remains unclear whether these findings reflect transcript per neuron, fewer neurons, or both. Distinguishing among alternatives has implications for understanding pathogenesis of, developing new treatments for, DLPFC dysfunction schizophrenia. Methods: To identify SST PV neurons postmortem human DLPFC, authors used fluorescent situ hybridization to...
To meet the high energy demands of brain function, cerebral blood flow (CBF) parallels changes in neuronal activity by a mechanism known as neurovascular coupling (NVC). However, which neurons play role mediating NVC is not well understood. Here, we identify mice and humans specific population cortical GABAergic that co-express nitric oxide synthase tachykinin receptor 1 (Tacr1). Through whole-tissue clearing, demonstrate Tacr1 extend local long-range projections across functionally...
Monocytes/macrophages are key cells in the pathogenesis of human cytomegalovirus (HCMV). Although HCMV infection monocytes is restricted to early events gene expression, productive has been demonstrated differentiated macrophages vitro. We examined cellular and cytokine components that essential for replication Concanavalin A-stimulated monocyte-derived (MDM). By negative selection, depletion CD8+ T lymphocytes, but not CD4+ CD19+ B cells, or CD56+ NK resulted a 60-70% reduction number...
ABSTRACT Endothelial cells (EC) have been implicated as constituting an important cell type in the pathogenesis of human cytomegalovirus (HCMV). Microvascular and macrovascular EC exhibit different biochemical functional properties depending on organ origin. Phenotypic differences between microvascular may alter ability these to support HCMV replication. In this study, we compared replication primary aortic (AEC) with that brain (BMVEC). An examination IE72, pp65, gB viral antigen expression...
The heterogeneity of γ-aminobutyric acid interneurons in the rodent neocortex is well-established, but their classification into distinct subtypes remains a matter debate. primate further complicated by less extensive database features these neurons and reported interspecies differences. Consequently, this study we characterized 8 different morphological types from monkey prefrontal cortex, 4 which have not been previously classified. These interneuron differed expression molecular markers...
Development of inhibition onto pyramidal cells may be crucial for the emergence cortical network activity, including gamma oscillations. In primate dorsolateral prefrontal cortex (DLPFC), inhibitory synaptogenesis starts in utero and synapse density reaches adult levels before birth. However, DLPFC, expression γ-aminobutyric acid (GABA) synapse-related gene products changes markedly during development until young age, suggesting a highly protracted maturation GABA function. Therefore, we...
Ribosome-associated vesicles are a novel, dynamic ER-derived organelle.