A5013491557- Insect Resistance and Genetics
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Entomopathogenic Microorganisms in Pest Control
- Insect-Plant Interactions and Control
- Insect Utilization and Effects
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Genetics and Neurodevelopmental Disorders
- Agricultural safety and regulations
- Invertebrate Immune Response Mechanisms
Tokyo University of Agriculture and Technology
2016-2024
Japan Society for the Promotion of Science
2021-2024
Bacillus thuringiensis produces Cry toxins, which are used as insecticides in sprays and transgenic crops. However, little is known about the function of toxin receptors mechanisms that determine their binding specificity activity. In this study, cRNAs Bombyx mori ABC transporter C2 (BmABCC2), toxin-binding region cadherin-like receptor (BtR175-TBR), or aminopeptidase N1 (BmAPN1) were injected into Xenopus oocytes, Cry1Aa-dependent cation-selective pore formation activities these analyzed...
Bacillus thuringiensis Cry toxins are insecticidal proteins used widely for pest control. They lethal to a restricted range of insects via specific interactions with insect receptors such as the ABC transporter subfamily members C2 (ABCC2) and C3 (ABCC3). However, it is still unclear how these different contribute susceptibility Cry1A toxins. Here, we investigated differences between silkworm (Bombyx mori) ABCC2 (BmABCC2_S) ABCC3 (BmABCC3) in mediating toxicity. Compared BmABCC2_S, BmABCC3...
Recent studies have suggested that ABC transporters are the main receptors of Cry toxins. However, many toxins not been identified. In this study, we used a heterologous cell expression system to identify Bombyx mori transporter subfamily C members (BmABCCs) function as for five active in Lepidopteran insects: Cry1Aa, Cry1Ca, Cry1Da, Cry8Ca, and Cry9Aa. All can use multiple ABCCs low-efficiency receptors, which induce cytotoxicity only at high concentrations. Surface plasmon resonance...
Cry toxins are insecticidal proteins produced by Bacillus thuringiensis (Bt). They used commercially to control insect pests since they very active in specific insects and harmless the environment human health. The gene encoding ATP-binding cassette subfamily A member 2 (ABCA2) was identified an analysis of Cry2A toxin resistance genes. However, we do not have direct evidence for role ABCA2 or why does cross other toxins. Therefore, performed two experiments. First, edited sequence Bombyx...
Little information is available regarding the region of Cry toxins involved in binding to their major receptors, ATP-binding cassette (ABC) transporters. We analyzed which Cry1Aa amino acid residues contribute Bombyx mori ABC transporter C2 (BmABCC2). Several two oxidized double-cysteine substitution mutant were made. In these, acids at distant positions on toxin loop α8 and 2 or 3 substituted with cysteine crosslinked. These mutants exhibited a marked reduction affinity BmABCC2, suggesting...