Roland Kerpes

ORCID: 0000-0002-1690-1917
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About
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Research Areas
  • Fermentation and Sensory Analysis
  • Fungal and yeast genetics research
  • Celiac Disease Research and Management
  • Meat and Animal Product Quality
  • Microbial Inactivation Methods
  • Microfluidic and Bio-sensing Technologies
  • Microbial Metabolites in Food Biotechnology
  • Digestive system and related health
  • Protein Hydrolysis and Bioactive Peptides
  • Food composition and properties
  • Consumer Attitudes and Food Labeling
  • Gene Regulatory Network Analysis
  • Olfactory and Sensory Function Studies
  • Fault Detection and Control Systems
  • Freezing and Crystallization Processes
  • Galectins and Cancer Biology
  • Biochemical Analysis and Sensing Techniques
  • Biofuel production and bioconversion
  • Eosinophilic Esophagitis
  • GDF15 and Related Biomarkers
  • Innovative Microfluidic and Catalytic Techniques Innovation
  • Cell Image Analysis Techniques
  • Magnetic and Electromagnetic Effects
  • Bacterial Genetics and Biotechnology
  • Animal Nutrition and Physiology

Technical University of Munich
2012-2024

Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2022

The high loss rate of bread is generally known to contribute the alarmingly numbers in worldwide food waste. Correct storage techniques are believed enable reduction preventable Therefore, influence parameters on staling and spoilage behavior German within limits common household methods was investigated this study. aim generate reliable data for using different (PE-layered microperforated paper bag, plastic fridge box) bridge gap between consumer’s needs scientific research questions....

10.3390/foods10010076 article EN cc-by Foods 2021-01-02

A novel millifluidic magnetophoresis approach can be used to fractionate yeast cells according their individual cell age. Old and young show different behavior in gene expression metabolome.

10.1039/d4lc00185k article EN cc-by Lab on a Chip 2024-01-01

Despite the fact that yeast is a widely used microorganism in food, beverage, and pharmaceutical industries, impact of viability age distribution on cultivation performance has yet to be fully understood. For detailed analysis fermentation physiological state, we introduced method magnetic batch separation isolate daughter mother cells from heterogeneous culture. By binding functionalised iron oxide nanoparticles, it possible separate chitin-enriched bud scars by way linker protein. This...

10.1002/biot.202200610 article EN cc-by Biotechnology Journal 2023-04-04

For industrial processes, a fast, precise, and reliable method of determining the physiological state yeast cells, especially viability, is essential. However, an increasing number processes use magnetic nanoparticles (MNPs) for cell manipulation, but their impact on viability assay itself unclear. This study tested Saccharomyces pastorianus ssp. carlsbergensis Pichia pastoris by comparing traditional colourimetric, high-throughput, growth assays with membrane fluidity. Results showed that...

10.1007/s00216-023-04676-w article EN cc-by Analytical and Bioanalytical Chemistry 2023-04-21

Food-derived peptides with an inhibitory effect on dipeptidyl peptidase IV (DPP-IV) can be used as additive treatment for type 2 diabetes. The potential of food depends technological protein hydrolysis and gastrointestinal digestion, the only act after intestinal resorption. malting a hydrolytic step availability these in grains has yet to investigated. In this study, quinoa was malted under systematic temperature, moisture, time variations. resulting malts, DPP-IV inhibition reached maximum...

10.1021/acs.jafc.4c00570 article EN cc-by Journal of Agricultural and Food Chemistry 2024-05-11

The brewing industry is facing an ever increasing challenge to become more cost-effective, while at the same time maintaining or improving product quality. Brewing with unmalted oats (Avena sativa L.) has potential reduce costs of raw materials. However, replacement malted barley can also adversely affect quality and processability mashes, worts, beers. In this study, (0–40%) was carried out in a 60-L pilot plant. impact various levels on mashing, lautering, fermentation performance...

10.1094/asbcj-2012-0708-01 article EN Journal of the American Society of Brewing Chemists 2012-06-01

Flow cytometry is a powerful tool for identifying and quantifying various cell markers, such as viability, vitality, individual age, at single-cell stages. However, autofluorescence marker fluorophore signals overlap low fluorescence intensities. Thus, these must be unmixed before determining the age fraction.A comparison was made between principal component regression (PCR) random forest (RF) to predict of Saccharomyces pastorianus var. carlsbergensis in flow cytometer. RF provided better...

10.1002/biot.202200091 article EN cc-by Biotechnology Journal 2022-11-03

The physiological and metabolic diversity of a yeast culture is the sum individual cell phenotypes. As well as environmental conditions, genetics, numbers divisions, major factor influencing characteristics age. A postcytokinesis bud scar on mother cell, benchmark in replicative life span, quantifiable indicator age, characterized by significant amounts chitin. We developed binding process for visualizing scars Saccharomyces pastorianus var. carlsbergensis using protein linker containing...

10.1039/d1ra05276d article EN cc-by-nc RSC Advances 2021-01-01

Abstract Gluten-free beer (GFB) was produced from gluten-containing barley malt wort by adding an enzyme-enriched extract to the wort, intended for patients with celiac disease. Nevertheless, aroma profile of such is unknown. The results indicated that treatment reduced 3-methylbutyl acetate, 2-methylbutyl ethyl hexanoate, 2-methylpropyl and butyrate when compared reference. Moreover, foam, free amino nitrogen, aging stability, color deviated significantly in GFB. Although values were below...

10.1007/s00217-022-04050-7 article EN cc-by European Food Research and Technology 2022-06-01

Consumer health concerns and regulatory policies lead to a growing demand for sugar-sweetened beverage alternatives. A reduced energy content can be achieved using artificial sweeteners, which often also convey metallic or bitter off-flavor. Therefore, the alteration of sweetness perception masking potential off-flavors are paramount improving sweet beverages. Trigeminal stimuli, such as capsaicin (spicy) menthol (cooling), have been used influence taste in food items, although their use...

10.3390/foods11182734 article EN cc-by Foods 2022-09-06

Abstract Downscaling the anaerobic fermentation in a microtiter plate (MTP) facilitates high throughput screening (HTS) applications. This study investigates impacts of MTP configurations (scale, shaking, and cover) on S. pastorianus beer compared to that shaking flask (SF) European Brewing Convention (EBC) tube regarding performances flavor attributes. The lager strains MTPs accelerated cells reproduction vitalization, sugar consumption, glycerol accumulation. microscale was closer SF but...

10.1007/s00217-022-04009-8 article EN cc-by European Food Research and Technology 2022-04-22
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