A5041675071- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- NF-κB Signaling Pathways
- Cellular transport and secretion
- Nitric Oxide and Endothelin Effects
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- Barrier Structure and Function Studies
- Neurological diseases and metabolism
- Liver physiology and pathology
- Immune cells in cancer
- Autophagy in Disease and Therapy
- Advanced Glycation End Products research
- Cellular Mechanics and Interactions
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Pancreatic function and diabetes
- Receptor Mechanisms and Signaling
- Hippo pathway signaling and YAP/TAZ
- Antimicrobial Peptides and Activities
- Psoriasis: Treatment and Pathogenesis
- Cytokine Signaling Pathways and Interactions
- Wnt/β-catenin signaling in development and cancer
- Immunodeficiency and Autoimmune Disorders
Kyushu University
2014-2025
Interleukin (IL)-17-producing T helper (Th17) cells are crucial for host defense against extracellular microbes and pathogenesis of autoimmune diseases. Here we show that the AP-1 transcription factor JunB is required Th17 cell development. Junb-deficient CD4+ able to develop in vitro into various subsets except Th17. The RNA-seq transcriptome analysis reveals Th17-specific gene expression program. mice completely resistant experimental encephalomyelitis, a Th17-mediated inflammatory...
The membrane-integrated NADPH oxidases DUOX1 and DUOX2 are recruited to the apical plasma membrane in epithelial cells release hydrogen peroxide, thereby playing crucial roles various functions such as thyroid hormone synthesis host defense. However, it has remained unknown about molecular mechanism for sorting of DUOX2. Here we show that correctly sorted via membrane-spanning DUOX maturation proteins DUOXA1 DUOXA2, respectively, when co-expressed MDCK cells. Impairment N-glycosylation...
ABSTRACT The small GTPases RhoA and Cdc42 their effector proteins play crucial roles in neutrophil chemotaxis. However, endogenous localization regulation of these have remained largely unknown. Here, we show, using a trichloroacetic acid fixation method, that are preferentially accumulated at the F‐actin‐rich leading edge (pseudopod) during chemotaxis human neutrophil‐like PLB‐985 cells response to chemoattractant C5a. Interestingly, enrichment is impaired by knockdown Cdc42, indicating...
The adhesion family of G protein-coupled receptors (GPCRs) is defined by an N-terminal large extracellular region that contains various adhesion-related domains and a highly-conserved GPCR-autoproteolysis-inducing (GAIN) domain, the latter which located immediately before canonical seven-transmembrane domain. These are expressed widely involved in functions including development, angiogenesis, synapse formation, tumorigenesis. GPR125 (ADGRA3), orphan GPCR, has been shown to modulate planar...
The nuclear protein IκBζ, comprising the N-terminal trans-activation domain and C-terminal ankyrin repeat (ANK) composed of seven ANK motifs, activates transcription a subset factor-κB (NF-κB)-dependent innate immune genes such as Lcn2 encoding antibacterial lipocalin-2. activation requires formation complex containing IκBζ NF-κB p50, factor that harbors DNA-binding Rel homology region but lacks domain, on promoter with canonical NF-κB-binding site (κB site) its downstream cytosine-rich...
The nuclear protein I κ B ζ activates transcription of a subset NF ‐ ‐dependent innate immune genes such as L cn2 encoding the antibacterial lipocalin‐2. functions coactivator via its interaction with p50, which contains DNA ‐binding R el‐homology domain but lacks transcriptional activation domain. However cis ‐regulatory elements involved in function have remained unknown. Here, we show that, although by itself is unable to associate promoter, interacts promoter p50 binding site ( site) and...
A single epithelial cell embedded in extracellular matrix (ECM) can proliferate to form an apical lumen-harboring cyst, whose formation is a fundamental step organ development. At early two-cell stage after division, the doublet typically displays "inverted" polarity, with and basolateral proteins being located ECM-facing cell-cell-contacting plasma membranes, respectively. Correct cystogenesis requires polarity reorientation, process containing protein endocytosis from ECM-abutting...
Transmembrane glycoproteins, synthesized at the endoplasmic reticulum (ER), generally reach Golgi apparatus in COPII‐coated vesicles en route to cell surface. Here, we show that bona fide nonglycoprotein Nox5, a transmembrane superoxide‐producing NADPH oxidase, is transported surface manner resistant co‐expression of Sar1 (H79G), GTP‐fixed mutant small GTPase Sar1, which blocks COPII vesicle fission from ER. In contrast, (H79G) effectively inhibits ER‐to‐Golgi transport glycoproteins...
Abstract The tight junction (TJ) in epithelial cells is formed by integral membrane proteins and cytoplasmic scaffolding proteins. former contains the claudin family with four transmembrane segments, while latter includes Par3, a PDZ domain-containing adaptor that organizes TJ formation. Here we show single membrane-spanning protein TMEM25 localizes to TJs binds Par3 via PDZ-mediated interaction its C-terminal tail. development during cell polarization accelerated depletion of TMEM25,...
Hepatocytes differ from columnar epithelial cells by their multipolar organization, which follows the initial formation of central lumen-sharing clusters polarized as observed during liver development and regeneration. The molecular mechanism for hepatocyte polarity establishment, however, has been comparatively less studied than those other cell types. Here, we show that tight junction protein Par3 organizes polarization via cooperating with small GTPase Cdc42 to target atypical kinase C...
Abstract The hydrogen peroxide (H 2 O )‐producing NADPH oxidase Nox4, forming a heterodimer with p22 phox , is expressed in variety of cells including those the heart to mediate adaptive responses cellular stresses such as hypoxia. Since Nox4 constitutively active, H production controlled by its protein abundance. Hypoxia‐induced expression observed various types and generally thought be regulated at transcriptional level. Here we show that hypoxia upregulates level Nox4‐catalyzed without...