Phenazines, secondary metabolites of fluorescent Pseudomonas, represent a group heterocyclic nitrogen-containing compounds showing broad spectrum antibiotic properties. Phenazines producing Pseudomonas species are studied extensively for their application in plant disease management. In this study, we examined the antifungal activity different indigenous isolates (Q16, B25 and PS2) against phytopathogenic fungus Alternaria tenuissima, which had infected cardoon (Cynara cardunculus L.,...

Anise (Pimpinella anisum L.) is an important medicinal spice plant that belongs to the family Apiaceae. seeds are rich in essential oils and this a reason why anise production Serbia has increased over last decade. During routine health inspection on collected from three localities province of Vojvodina (Mošorin, Veliki Radinci Ostojićevo) during 2012 2013, it was found out Fusarium spp. were commonly observed fungi. The presence fungion seed samples ranged 3.75-13.75%. aim study isolate...

Thirty-six strains of Xanthomonas campestris pv. (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into mesophilic tissue cabbage leaves. All Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35?C, produced levan, H2S indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin esculin show tolerance 0.1...

Talaromyces minioluteus is one of the important species genus Talaromyces, which has cosmopolitan distribution and encountered on a wide range different habitats. This not been considered as an plant pathogen, even though it isolated from various hosts. Fruits vegetables with Penicillium-like mold symptoms were collected 2015 to 2017 markets in Serbia. Isolates originating quince, tomato, orange fruits, onion bulbs, potato tubers identified characterized morphological, physiological,...

In September 2010, leaves of oilseed rape (Brassica napus L.) with v-shaped, necrotic lesions on the leaf margins surrounded by yellow halos were collected. Symptoms observed domestic cultivar Slavica (IFVC, Novi Sad) located in Bačka region, Vojvodina, Serbia, from a 3-ha field. Average disease incidence 3-month-old plants was 45% (15 to 75%). Diseased rinsed sterilized distilled water (SDW) and dried at room temperature for isolations. Leaf sections taken margin tissue macerated SDW...

Pseudomonas syringae is a widespread and economically important plant pathogen, one found on number of hosts, including fruit trees, field crops, vegetables, ornamental plants. This bacterium has been experimentally identified as parasite pear, apple, apricot, peach, cherry, sour plum, raspberry. The present study was designed to establish differences between strains isolated from trees in Serbia. pathogenic biochemical characteristics isolates were studied. BOX-PCR method used generate...

Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar milk tween agar used the bacterium whole seed extract. Colonies of yellow, mucoid convex on these media zones hydrolysis formed around them. The pathogenicity strains was confirmed by stem inoculation young plants. Identification proved using rapid methods (ELISA...

A multidisciplinary approach was used to compare phenolic composition, radical scavenging and antimicrobial activity of propolis samples from different geographical localities, plant resin against various microorganisms. Using UHPLC‐qqqMS quantitative analysis, 28 compounds were determined. Caffeic p‐coumaric acids identified as main in poplar samples, except Russia (P6) China (P7). Radical (applying DPPH spectrophotometric assay) showed the highest Serbian (40·51%) Chinese (53·21%) samples....

Indoleacetic acid (IAA) producing Pseudomonas isolates from the rhizosphere of maize (Q4 and Q20), alfalfa (Q1 Q16) wild red clover (B25) were selected for investigation their effect on biological vitality potato seed tubers. The production IAA ranged 4.09 to 15.9 ? gmL- 1 after 24h cultivation 4.08 26.4 gmL-1 48h cultivation. molecular comparison by RAPD analysis also was done. patterns spp. obtained BC318, AF14, SPH AP 10 primers demonstrated suitability method in distinguishing a high...

Abstract Plants of alfalfa ( M edicago sativa ) exhibiting general stunting, proliferation and phyllody associated with leaf yellowing reddening were observed in three localities C entral S erbia. Phytoplasma strains belonging to 16 rIII‐ B r XII ‐ A groups detected identified by RFLP sequence analysis DNA . Stolbur phytoplasma tuf gene showed the presence T uf AY ‐b‐type subgroup 80% symptomatic samples. This is first report III affecting

Summary The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. dSPE carried out with 1% acetic acid acetonitrile/water a mixture magnesium sulphate, sodium chloride citrate. analytes were separated on Zorbax XDB C18 column using methanol/water as mobile phase gradient mode. detection done tandem masss spectrometry (MS/MS) positive ESI. tropane alkaloids exhibited excellent linearity range 2-20 µg/kg LOQ 5 maize. extraction...

Pot marigold (Calendula officinalis L.) is native to southern Europe. Compounds of flowers exhibit anti-inflammatory, anti-tumor-promoting, and cytotoxic activities (4). In Serbia, pot cultivated as an important medicinal ornamental plant. Typical phyllody, virescence, proliferation axillary buds, witches' broom symptoms were sporadically observed in 2011 Pancevo plantation, Serbia (44°51'49″ N, 20°39'33″ E, 80 m above sea level). Until 2013, the number uniformly distributed affected plants...

Halo blight, caused by Pseudomonas savastanoi pv.phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia.Use of pathogen-free seeds one the most effective control measures against this disease.The aim study was evaluate a detection method for Psp untreated (23 genotypes) from commercial crops grown within Serbia.Detection pathogen made plating onto modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums soaked...

Antibiotic production by plant-associated microorganisms represents an environmentally compatible method of disease control in agriculture. However, a vide application bacterial strains needs careful selection and genetic characterization. In this investigation, selected Pseudomonas were characterized rep-PCR methods using ERIC (GTG)5 primers, partial 16S rDNA sequence analysis. None produced homoserine lactones (C4, C6, C8) as quorum sensing signal molecules. Very poor phenazines no...

The essential oil of Lavandula stoechas was examined by GC and GC-MS. Discs (5 mmi.d.) the tested fungi ( Alternaria alternata, Fusarium oxysporum Botritys cinerea) were inoculated separately onto each assay plate incubated at 25 o C for 7 days. yield dried parts (v/dw) obtained hydro-distillation 2.9%. Thirty-two compounds representing 98.3% determined. Linalool (49.9%), linalyl acetate (14.4%), lavandulyl (5.7%), α-terpineol (5.6%), terpinene-4-ol (5.1%), lavandulol (3.7%), E)-β-ocimene...

Blueberries (Vaccinium corymbosum) are among the healthiest fruits due to their high antioxidant content. The total growing area of blueberries in Serbia ranges from 80 90 ha. A phytoplasma-like disease was observed for first time during July 2009 three blueberry cultivars (Bluecrop, Duke, and Spartan) grown central Serbia, locality Kopljare (44°20'10.9″ N, 20°38'39.3″ E). Symptoms yellowing reddening were on upper leaves proliferating shoots, similar those already described (4). There...

During a survey carried out from 2005 to 2010, reddening, stunting and proliferation of axillary shoots or witches' broom symptoms were observed on Echinacea purpurea in Pancevo Indjija, Serbia.The association phytoplasma with these has been detected by electron microscopic observations PCR followed nested using 16S rRNA gene specific primers.Further, 16SrXII-A group (stolbur) phytoplasmas identified RFLP analysis TruI, KpnI AluI restriction endonucleases.

Chicory (Cichorium intybus, Asteraceae) is a typical Mediterranean plant indigenous to Europe, western Asia, Egypt, and North America (3). It commonly consumed as fresh vegetable in salads. In rural areas of Serbia it grows weed crops, but used folk medicine treat skin disorders due its antihepatotoxic activity Methanol extracts chicory leaves showed moderate antibacterial against enteric bacteria A phytoplasma-like disease, expressed proliferation shoots flowers, was observed on wild plants...

Evening primrose (Oenothera biennis L.) is a biennial medicinal, edible, and ornamental plant species. It has attracted great interest for its seed oil that contains gamma linolenic acid, thus distinguishing this as main commercial source of essential fatty acid (4). This species been grown permanent member medicinal collection established near Backi Petrovac (northern Serbia) 22 years. The first disease symptoms were recognized red spots on leaf rosette in July 2011, spreading gradually...