A5079416014- Fungal and yeast genetics research
- Receptor Mechanisms and Signaling
- Enzyme-mediated dye degradation
- Adenosine and Purinergic Signaling
- Bacterial Genetics and Biotechnology
- Metabolism, Diabetes, and Cancer
- Plant nutrient uptake and metabolism
- Microtubule and mitosis dynamics
- Growth Hormone and Insulin-like Growth Factors
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Peroxisome Proliferator-Activated Receptors
- Pharmacological Receptor Mechanisms and Effects
- HER2/EGFR in Cancer Research
- Neuropeptides and Animal Physiology
- Animal Genetics and Reproduction
- Microbial Natural Products and Biosynthesis
- Parkinson's Disease Mechanisms and Treatments
- Plant Molecular Biology Research
- Protein Kinase Regulation and GTPase Signaling
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Transgenic Plants and Applications
- Genetics and Neurodevelopmental Disorders
Boehringer Ingelheim (Austria)
2001-2018
Boehringer Ingelheim (Germany)
2000
North Carolina State University
1992
Bio Forschung Austria
1983-1991
University of Vienna
1983-1991
Osaka University
1989
Polish Academy of Sciences
1983
Expression of the CTA1 gene Saccharomyces cerevisiae, encoding catalase A, peroxisomal this yeast, is sensitive to glucose repression. A DNA fragment cloned as a multicopy plasmid suppressing repression transcription was demonstrated contain ADR1 gene. Multiple copies increased formation not only on 10% glucose, but also ethanol medium and in presence oleic acid, an inducer peroxisome proliferation. Compared with wild-type cells, adr1 null mutants produced by disruption exhibit reduced...
We have constructed a Xenopus oocyte cDNA library in Saccharomyces cerevisiae expression vector and used this to isolate genes that can function yeast cells suppress the temperature sensitive [corrected] defect of cdc15 mutation. Two maternally expressed cDNAs which fulfill these conditions been isolated. One clones encodes N-ras. In contrast RAS genes, N-ras rescues Moreover, overexpression S. does not activate RAS-cyclic AMP (cAMP) pathway; rather, it results decreased levels intracellular...
The catalase T structural gene of Saccharomyces cerevisiae was cloned by functional complementation a mutation causing specific lack the enzyme (cttl). Catalase T-deficient mutants were obtained UV mutagenesis an S. strain bearing cas1 mutation, which causes insensitivity to glucose repression. Since second protein cerevisiae, A, is completely repressed on 10% glucose, mutant colonies could be detected under such conditions. A cttl transformed with library in plasmid YEp13. Among T-positive...
We have undertaken the production of recombinant soluble Fc epsilon receptor II (Fc RII) as a secretory protein, but not cleavage product membrane-bound receptor. Several plasmid constructs containing sequence were prepared. Only chimeric gene sequences encoding IL-6 signal peptide and moiety RII could be expressed in Xenopus laevis oocytes CHO cells, resulting secretion RII. The was also produced yeast expression system. NH2-terminal analysis generated by demonstrated correct leader...
ABSTRACT Protein ubiquitylation is a dynamic post-translational modification that can be reversed by deubiquitylating enzymes (DUBs). It unclear how the small number (∼100) of DUBs present in mammalian cells regulate thousands different events. Here, we analysed annotated transcripts human and found ∼300 ribosome-associated as protein coding, which thus increases total DUBs. By using USP35, poorly studied DUB, case study, provide evidence alternative isoforms contribute to functional...
Expression of the CTA1 gene Saccharomyces cerevisiae, encoding catalase A, peroxisomal this yeast, is sensitive to glucose repression. A DNA fragment cloned as a multicopy plasmid suppressing repression transcription was demonstrated contain ADR1 gene. Multiple copies increased formation not only on 10% glucose, but also ethanol medium and in presence oleic acid, an inducer peroxisome proliferation. Compared with wild-type cells, adr1 null mutants produced by disruption exhibit reduced...
The neurokinin receptor family is known to modulate phospholipase C activity. In order find new compounds modulating the activity of these receptors we have developed a cellular screening system that measures biological coupled IP3/DAG signal transduction pathway via transcriptional activation reporter gene. For establishment test cell lines line A20, stably transformed with luciferase gene under control promoter containing TPA response elements (TRE), which did not respond agonists, was...
The catalase T structural gene of Saccharomyces cerevisiae was cloned by functional complementation a mutation causing specific lack the enzyme (cttl). Catalase T-deficient mutants were obtained UV mutagenesis an S. strain bearing cas1 mutation, which causes insensitivity to glucose repression. Since second protein cerevisiae, A, is completely repressed on 10% glucose, mutant colonies could be detected under such conditions. A cttl transformed with library in plasmid YEp13. Among T-positive...
We have constructed a Xenopus oocyte cDNA library in Saccharomyces cerevisiae expression vector and used this to isolate genes that can function yeast cells suppress the temperature sensitive [corrected] defect of cdc15 mutation. Two maternally expressed cDNAs which fulfill these conditions been isolated. One clones encodes N-ras. In contrast RAS genes, N-ras rescues Moreover, overexpression S. does not activate RAS-cyclic AMP (cAMP) pathway; rather, it results decreased levels intracellular...
Propentofylline is neuroprotective in vivo, but its mechanism of action not completely understood. Previously, propentofylline was shown to block adenosine transport processes, inhibit three receptor subtypes, and cAMP phosphodiesterase. We tested the effect on function Chinese hamster ovary (CHO) cells transfected with human A1, A2A, or A2B receptors a luciferase reporter gene under control promoter sequence containing several copies response element. investigated concentration-dependent...
Propentofylline is neuroprotective in vivo, but its mechanism of action not completely understood. Previously, propentofylline was shown to block adenosine transport processes, inhibit three receptor subtypes, and cAMP phosphodiesterase. We tested the effect on function Chinese hamster ovary (CHO) cells transfected with human A 1 , 2A or 2B receptors a luciferase reporter gene under control promoter sequence containing several copies response element. investigated concentration-dependent...
Abstract Background: Inhibitors of apoptosis proteins (IAPs) regulate cellular by interfering with the proteolytic activities caspases. IAP inhibitors (SMAC mimetics) have been developed to restore defective that characterizes many tumour cells. Emerging evidence demonstrates IAPs are critical components immune-modulatory pathways control innate and adaptive immunity. Accordingly, SMAC mimetics hold promise both inducing cell killing stimulating immune system recognize eliminate dying Here...