Lentil quantitative trait loci (QTL) related to plant structure (branches at first node, height of total number branches, height), growth habit (flowering time, pod dehiscence) and yield (number seeds, seed weight, diameter) were located using a F2 population 113 individuals derived from the intersubspecific cross Lens culinaris ssp. L. c. orientalis. Several traits found be significantly correlated. Using interval composite mapping 23 QTL for nine located. No was identified F3 produced. Six...

Usage of high-throughput sequencing approaches allow for the generation and characterization reference transcriptome datasets that support gene-based marker discovery, which in turn can be used to build genetic maps among other purposes. We have obtained a assembly including 49,453 genes lentil (Lens culinaris Medik.) cultivar Alpo using RNAseq methodology. This was as obtain 6,306 quality polymorphic markers (SNPs short indels) analyzing genotype data from RIL population at F7 derived...

Abstract Avena barbata, an autotetraploid grass, is much more widely adapted than hirtula, its diploid ancestor. We have determined the 14-locus genotype of 754 and 4751 tetraploid plants from 10 50 Spanish sites, respectively. Allelic diversity greater in (52 alleles) (38 alleles): extra alleles were present nonsegregating heteroallelic quadriplexes. Seven loci monomorphic for same allele (genotypically 11) all populations diploid: five these also 1111) whereas two each formed a quadriplex...

Abstract Spanish explorers and colonists inadvertently started a massive experiment in evolutionary genetics when they accidentally introduced Avena barbata to California from Spain during the seventeenth eighteenth centuries. Assays of Californian gene pools this species for 15 loci show that present day pool, particularly Southwestern Spain, is identical or virtually five closely similar nine loci. Despite their allelic single-locus genotypic compositions, present-day are differently...

Electrophoretic banding patterns were determined for nine enzyme systems (IPO, PGM, PGI, LAP, GOT, EST, PHOS, MDH, CPX) in four populations of Secale cereale L. from widely different geographical areas, and one population S. vavilovii Grossh. was found to be extensively variable invariant these systems. Formal genetic studies polymorphic zones revealed that each zone under single locus control. Mating system based on loci indicated 8% self-fertilization occurred field conditions a cereale,...

Abstract Background and Aims Flowering time is important due to its roles in plant adaptation different environments subsequent formation of crop yield. Changes light quality affect a range developmental processes including flowering time, but little known about quality-induced control lentil. This study aims investigate the genetic basis for differences response Methods We explored variation caused by changes red/far-red-related lentil interspecific recombinant inbred line (RIL) population...

Most known plant disease-resistance genes (R genes) include in their encoded products domains such as a nucleotide-binding site (NBS) or leucine-rich repeats (LRRs). Sequences with unknown function, but encoding these conserved domains, have been defined resistance gene analogues (RGAs). The motifs within NBS make it possible to use degenerate primers and PCR isolate RGAs. We used deduced from the domain of NBS-LRR proteins amplify genomic sequences Lens species. Fragments approximately...

Abstract Fourteen isozymatk loci have been analyzed in 23 samples of populations belonging to genus Secale by starch gel electrophoresis. The allelic frequencies for these also estimated and used calculating genetic identity values between populations. Isozymatic data agree with the hypothesis that there are four species Secale: S. cereale, montanum, silvestre , vavilovii. characteristics growth outbreeding habits, isozymatic variability along previously known cytogenetic data, maintain...

A total of 122 accessions different wild and cultivated Pisum sp. were analysed using retrotransposon-based insertion polymorphisms (RBIP) markers. The materials included (landraces cultivars) from the World core collection John Innes Centre (JI) representing all generally recognized taxa, landraces Spanish collection, commercial pea cultivars largely sown in Spain. overall polymorphism detected by RBIP marker was high accessions, except two pairs, could be distinguished their pattern....

Powdery mildew is a widespread fungal plant disease that can cause significant losses in many crops. Some MLO genes (Mildew resistance locus O) have proved to confer durable powdery several species. Resistance granted by the gene family members has prompted an increasing interest characterizing these and implementing their use breeding. Lentil (Lens culinaris Medik.) widely grown food legume almost exclusively consumed as dry seed with average world production of 4.5 million tons. causes...

Lentil crosses (Lens culinaris ssp. and L. c. orientalis) were carried out in the greenhouse field, effects of genotype some environmental conditions on crossing success assessed. In fall, independent variables “Male” “Hour” influenced pod seed set per pollinated flower “Temperature” affected number pod. Some genotypes different cross combinations better under conditions. Seed best inter-sub-specific combination (Lupa × averaged 55.2%, while for other intra average ranged between 3.6 to...

Pseudomonas syringae pv. causes extensive yield losses in the pea crop worldwide, although there is little information on its host specialization and interactions with pea. A collection of 88 putative P. strains (including 39 isolated from pea) was characterized by repetitive polymerase chain reaction (rep-PCR), multilocus sequence typing (MLST), syrB amplification evaluated for pathogenicity virulence. rep-PCR data grouped into two groups (1B 1C) together other hosts; a third group (1A)...

Frost is one of the main abiotic stresses limiting plant distribution and crop production. To cope with stress, plants evolved adaptations known as cold acclimation or chilling tolerance to maximize frost tolerance. Cold a progressive acquisition freezing by subjected low non-freezing temperatures which subsequently allows them survive exposure frost. Lentil cool season grain legume that challenged winter in some areas its cultivation. better understand genetic base differential gene...

Lentils (Lens sp.) are one of the main sources protein for humans in many regions, part because their rusticity allows them to withstand semi-dry climates and tolerate a wide spectrum pests. Both also highly sought-after attributes face climate change. Wild accessions, rather than cultivated varieties, typically holders most influential alleles traits. However, genomic transcriptomic research conducted lentils has been carried out on commercial accessions (L. culinaris), while wild relatives...

The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from libraries based on hybridization-capture repeat regions biotin-conjugated oligonucleotids non-radioactive colony hybridization was carried out. PCR products 200 800 bp were obtained cloned. Of 60 clones sequenced, 21 yielded with n values equal or higher than six....

Convicilins, a set of seed storage proteins, differ from vicilins, related group mainly because the presence N-terminal extension, an additional sequence amino acids in corresponding to first exon. Convicilins have been described only species legume tribe Vicieae. One or two genes for convicilins identified most this tribe. The genus Pisum is main exception, since its species. Thirty-four new convicilin gene sequences 29 different (Lathyrus, Lens, Pisum, and Vicia spp.) analyzed here....

We have applied a multivariate log-linear technique to the analysis of interlocus allelic associations among 14 allozyme loci in sample 4011 plants from 42 Spanish populations Avena barbata. The fell into three natural groups five, and four loci. five first group are invariant, or nearly so, throughout range species. genetic organization this set is defined by single five-locus genotype; each allele predominant genotype "wild-type" that contributes favorably adaptedness all single-locus...