A5023712149- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Enterobacteriaceae and Cronobacter Research
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Bacterial Infections and Vaccines
- Antifungal resistance and susceptibility
- Medical Device Sterilization and Disinfection
- Tuberculosis Research and Epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Antibiotic Use and Resistance
- Mycobacterium research and diagnosis
- Chemical Reactions and Isotopes
- Urinary Tract Infections Management
- Legionella and Acanthamoeba research
- Bacillus and Francisella bacterial research
- Salmonella and Campylobacter epidemiology
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Peptidase Inhibition and Analysis
- Infective Endocarditis Diagnosis and Management
- Microbial infections and disease research
- Antibiotics Pharmacokinetics and Efficacy
- Infectious Diseases and Mycology
- Polish socio-economic development
National Institute of Public Health
2010-2025
Wojskowy Instytut Higieny i Epidemiologii
2002-2015
Państwowa Wyższa Szkoła Zawodowa w Ciechanowie
2013
SGH Warsaw School of Economics
2013
University of Amsterdam
2008
University of Manchester
2007
ABSTRACT Phagocytic cells form the first line of defense against infections by human fungal pathogen Candida albicans . Recent in vitro gene expression data suggest that upon phagocytosis macrophages, C. reprograms its metabolism to convert fatty acids into glucose inducing enzymes glyoxylate cycle and acid β-oxidation pathway. Here, we asked whether β-oxidation, a metabolic pathway localized peroxisomes, is essential for virulence constructing two double deletion strains: pex5Δ/pex5Δ...
Summary Previously, we have shown that PDE2 is required for hyphal development and cell wall integrity in Candida albicans. In the present study, investigated effects of its deletion by genome‐wide transcriptome profiling. Changes expression levels genes involved metabolism, transcription, protein nucleic acids synthesis, as well stress responses, membrane biogenesis, adherence virulence been observed. By comparing these changes with previously reported profiles pde2 Δ mutants Saccharomyces...
The glyoxylate cycle, a metabolic pathway required for generating C4 units from C2 compounds, is an important factor in virulence, both animal and plant pathogens. Here, we report the localization of key enzymes this isocitrate lyase (Icl1; EC 4.1.3.1) malate synthase (Mls1; 2.3.3.9), human fungal pathogen Candida albicans. Immunocytochemistry combination with subcellular fractionation showed that Icl1 Mls1 are localized to peroxisomes, independent carbon source used. Although lack consensus...
A Klebsiella pneumoniae epidemic strain that coproduced carbapenemase KPC-2 (K. 2) and 16S rRNA methylase ArmA has emerged in Poland. Four nonduplicate isolates from patients a hospital Warsaw, Poland, were found to carry the bla(KPC-2) armA genes on ca. 50-kb 90-kb plasmids, respectively. Tn4401 with 100-bp deletion variable region was detected all isolates. XbaI pulsed-field gel electrophoresis (PFGE) revealed 93.2% similarity of All resistant carbapenems 4,6-disubstituted 2-deoxystreptamines.
Salmonellosis remains the second most common zoonosis in Europe. Resistance to fluoroquinolones (FQs) Salmonella has been increasing worldwide, with WHO considering FQ-resistant spp. as high-priority pathogens. The aim of this study was a retrospective analysis molecular mechanisms FQ resistance, detected among clinical ciprofloxacin-resistant enterica belonging serotypes. whole genome sequences (WGS) tested isolates were also analysed for occurrence other antimicrobial resistance...
We characterized 17 clinical isolates of Klebsiella pneumoniae producing 16S rRNA methylase ArmA. The originated in Poland from 2002 to May 2010 and encompassed four XbaI-PFGE clusters. All the were resistant amikacin, gentamicin kanamycin (MIC range: 256-1024 mg l(-1)) carried armA gene on a large plasmid approximately 90 or 130 kb 15 2 isolates, respectively. was found ~10 ClaI restriction fragment flanked by same elements as Tn1548. bla(CTX-M) for CTX-M-type extended-spectrum β-lactamase....
Aminoglycosides are a group of antimicrobial agents still the most commonly used in treatment life-threatening bacterial infections human and animals. The emergence spread 16S rRNA methylases, which confer high-level resistance to majority clinically relevant aminoglycosides, constitute major public health concern.Our goal was evaluate distribution methylases among different species Enterobacteriaceae during five month-long survey tertiary hospital Warszawa, Poland.In survey, total 1770...
Salmonella Kentucky belongs to zoonotic serotypes that demonstrate the high antimicrobial resistance and multidrug (including fluoroquinolones) is an emerging problem. To best of our knowledge, clinical S. strains isolated in Poland remain undescribed.Eighteen collected years 2018-2019 were investigated. All tested for susceptibility 11 antimicrobials using disc diffusion E-test methods. Whole genome sequences analysed genes, mutations, presence structure SGI1-K (Salmonella Genomic Island...
Abstract Background Transrectal ultrasound-guided prostate biopsy (TRUS-Bx) is considered an essential urological procedure for the histological diagnosis of cancer. It is, however, a “contaminated” which may lead to infectious complications. Recent studies suggest significant share fluoroquinolone-resistant rectal flora in post-biopsy infections. Methods The molecular mechanisms fluoroquinolone resistance, including PMQR (plasmid-mediated quinolone resistance) as well mutation QRDRs...
Background. Plasmid-mediated extended-spectrum β-lactamases (ESBLs), 16S rRNA methylases and quinolone resistance mechanisms (PMQRs) are well-known agents conferring to more than 1 antimicrobial in its group. The accumulation of these poses, therefore, a serious risk public health.Objectives. objective this study was investigate the presence common ß-lactamases Qnr-producing Enterobacteriaceae their genetic relatedness.Material Methods. We examined 18 isolates (Klebsiella pneumoniae n = 8,...
Fluoroquinolones (FQs) are recommended as prophylaxis for men undergoing transrectal prostate biopsy (TRUS-Bx). Recent studies suggest a significant share of FQ-resistant rectal flora in post-TRUST-Bx infections.435 Enterobacterales isolates from 621 patients attending 12 urological departments Poland were screened by PCR PMQR genes. PMQR-positive tested quinolone susceptibility and investigated whole genome sequencing (WGS) methods.In total, 32 (7.35%) E. coli strains with ciprofloxacin MIC...
The aim of this study is to compare the epidemiological situation legionellosis in Poland 2018-2021 prior years, taking into account impact COVID-19 pandemic 2020-2021.The assessment based on national surveillance data published annual bulletin "Infectious Diseases and Poisons Poland" from 2013 2021, as well Legionnaires' disease case reports collected sent Department Epidemiology Infectious Surveillance NIPH NIH - NRI by sanitary stations or submitted EpiBase.In Poland, both (an acute...