A5098667594- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Medicinal Plants and Neuroprotection
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Cholinesterase and Neurodegenerative Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
University of Oxford
2024
Abstract Astrocytes are increasingly appreciated to possess underestimated and important roles in modulating neuronal circuits. striatum can regulate dopamine transmission by governing the extracellular tone of axonal neuromodulators, including GABA adenosine. However, here we reveal that striatal astrocytes occupy a cell type-specific anatomical functional relationship with cholinergic interneurons (ChIs), through which they rapidly excite ChIs govern release via nicotinic acetylcholine...
SUMMARY Astrocytes are increasingly thought to have underestimated and important roles in modulating neuronal circuits. striatum can regulate dopamine transmission by governing the extracellular tone of axonal neuromodulators, including GABA adenosine. However, here we reveal that striatal astrocytes occupy a cell type-specific anatomical functional relationship with cholinergic interneurons (ChIs), through which they rapidly excite ChIs govern release via nicotinic acetylcholine receptors...
This protocol is to assess whether optogenetically activating astrocytes affects the dopamine concentration released following electrical stimulation.
This collection contains seven protocols detailing methods used in Stedehouder, J. and Roberts, B.M. et al (2024) Rapid modulation of striatal cholinergic interneurons dopamine release by satellite astrocytes.
The following protocol outlines the steps necessary for intracranial injections of viral vectors in dorsal or ventral striatum mice. These are generalisable different viruses and injection sites, however care must be taken to optimise coordinates virus titre.
This collection contains eight protocols detailing methods used in Stedehouder, J., Roberts, B.M., Raina, S.et al.Rapid modulation of striatal cholinergic interneurons and dopamine release by satellite astrocytes.Nat Commun15, 10017 (2024).
This protocol describes how to image GRAB Acetylcholine (ACh) sensor expression in ex vivo mouse striatal slices, and measure changes fluorescence signals resulted from electrically-evoked ACh release.
This protocol details confocal image acquisition steps and the analysis pipeline used to calculate inter-soma distance between cholinergic interneurons/pMSNs their nearest astrocyte. The partner Protocol: Immunofluorescent Labelling of Post-Mortem Rodent Brain Tissue describes how label along with striatal astrocytes in PFA perfusion-fixed, 50-µm thick, post-mortem rodent brain tissue.