A5012462860- Insect Resistance and Genetics
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- CRISPR and Genetic Engineering
- Genetically Modified Organisms Research
- Viral Infectious Diseases and Gene Expression in Insects
- Fish Ecology and Management Studies
- Marine and fisheries research
- Bacillus and Francisella bacterial research
- Ecology and Vegetation Dynamics Studies
- Child Welfare and Adoption
- Environmental Toxicology and Ecotoxicology
- Transportation Systems and Logistics
- Insect and Arachnid Ecology and Behavior
- Regional Socio-Economic Development Trends
- Plant Virus Research Studies
- Geochemistry and Elemental Analysis
- Genetics, Bioinformatics, and Biomedical Research
- Family and Disability Support Research
- Botany, Ecology, and Taxonomy Studies
- Heavy metals in environment
- Recycling and Waste Management Techniques
- Animal Genetics and Reproduction
- Animal testing and alternatives
- Environmental DNA in Biodiversity Studies
Bayer (United States)
2020-2021
Climate Central
2020
Monsanto (United States)
2011-2017
Florida International University
2008-2009
RNA interference (RNAi) has previously been shown to be effective in western corn rootworm (WCR, Diabrotica virgifera LeConte) larvae via oral delivery of synthetic double-stranded (dsRNA) an artificial diet bioassay, as well by ingestion transgenic plant tissues engineered express dsRNA. Although the RNAi machinery components appear conserved Coleopteran insects, key steps this process have not reported for WCR. Here we characterized sequence events that result mortality after a dsRNA...
The sequence specificity of the endogenous RNA interference pathway allows targeted suppression genes essential for insect survival and enables development durable efficacious insecticidal products having a low likelihood to adversely impact non-target organisms. spectrum activity 240 nucleotide (nt) dsRNA targeting Snf7 ortholog in Western Corn Rootworm (WCR; Diabrotica virgifera virgifera) was characterized by selecting testing insects based upon their phylogenetic relatedness WCR. Insect...
MON 87411 maize, which expresses DvSnf7 RNA, was developed to provide an additional mode of action confer protection against corn rootworm (Diabrotica spp.). A critical step in the registration a genetically engineered crop with insecticidal trait is performing ecological risk assessment evaluate potential for adverse effects. For 87411, plan that met specific goals by characterizing routes and levels exposure, testing representative functional taxa would be directly or indirectly exposed...
Two primary use patterns exist for dsRNA-based products crop protection: in planta produced dsRNA such as a genetically engineered (GE) crop; and topically applied spray application. To enable effective environmental risk assessments these products, must be successfully measured relevant compartments (soil, sediment, surface water) to provide information on potential exposure. This perspective reviews results from numerous fate degradation studies with unformulated dsRNAs demonstrate the...
Environmental risk assessments (ERA) support regulatory decisions for the commercial cultivation of genetically modified (GM) crops. The ERA terrestrial agroecosystems is well-developed, whereas guidance GM crops in aquatic ecosystems not as well-defined. purpose this document to demonstrate how comprehensive problem formulation can be used develop a conceptual model and identify potential exposure pathways, using Bacillus thuringiensis (Bt) maize case study. Within formulation, insecticidal...
Abstract The honey bee ( Apis mellifera L.) is the most important managed pollinator species worldwide and plays a critical role in pollination of diverse range economically crops. This to agriculture historically has been used as surrogate for pollinators evaluate potential adverse effects conventional, biological, microbial pesticides, well genetically engineered plants that produce pesticidal products. As part ecological risk assessment MON 87411 maize, which expresses double‐stranded RNA...
The spectrum of insecticidal activity Cry51Aa2.834_16 protein targeting hemipteran and thysanopteran insect pests in cotton was characterized by selecting screening multiple pest non-pest species, based on representation ecological functional groups, taxonomic relatedness (e.g. relationship to species where observed), availability for effective testing. Seven invertebrate orders, comprising 12 families 17 representative were screened susceptibility and/or the ability protect against feeding...
In recent years, corn rootworm (CRW)-resistant maize events producing two or more CRW-active Bt proteins have been commercialized to enhance efficacy against the target pest(s) by providing multiple modes of action (MoA). The hybrid MON 87411 has developed that produces Cry3Bb1 protein (hereafter Cry3Bb1) and expresses a RNAi-mediated MoA also targets CRW. As part an environmental risk assessment for 87411, potential interaction between DvSnf7 RNA DvSnf7) was assessed in 12-day diet...
Genetically modified (GM) crops have been cultivated safely for more than 25 years, and the framework conducting an environmental risk assessment (ERA) is well-established. Currently, there alignment of broadly-stated protection goals across global jurisdictions; however, a lack consistency data that are required by regulators. Risk assessors over years collective experience assessing safety GM conducted hundreds ERAs to evaluate crop safety. This provides scientific basis help determine...
Laboratory and field data generated on genetically modified (GM) plants in one country can inform the environmental risk assessment support regulatory decision-making for GM being cultivated another country. Well-designed studies that test clear hypotheses follow well-established methods allow conclusions to be made about potential effects from cultivation of a plant relative its conventional counterparts. Following principle transportability, if no biologically relevant differences between...
The insidious flower bug, Orius insidiosus (Say) (Heteroptera: Anthocoridae) is an important surrogate species for assessing potential effects of plant-incorporated protectants (PIPs) on nontarget heterotrophic predators. In this study, a continuous dietary exposure system was optimized by the effect diet composition and age survival development nymphs O. insidiosus. Greater than 85% control acceptable rate from nymph hatching to adult achieved using 5-d-old at test initiation bee...
The responsiveness of insects to oral delivery insecticidal dsRNA has been shown be dependent on length and sequence match. Previous work with the western corn rootworm (WCR, Diabrotica virgifera virgifera; Coleoptera: Chrysomelidae) demonstrated that at least one ≥21 nt match must present in DvSnf7 approximately ≥60 base-pairs (bp) for activity. Further data is needed activity <21 matches along characterization relationship between number matches. To characterize sequence-activity further,...