A5035481101- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Photochemistry and Electron Transfer Studies
- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Protein Interaction Studies and Fluorescence Analysis
- Electron Spin Resonance Studies
- Spectroscopy and Quantum Chemical Studies
- Lanthanide and Transition Metal Complexes
- Saffron Plant Research Studies
- TiO2 Photocatalysis and Solar Cells
- Magnetism in coordination complexes
- Radical Photochemical Reactions
- Molecular Sensors and Ion Detection
- Photosynthetic Processes and Mechanisms
- Chromium effects and bioremediation
- Phytochemicals and Antioxidant Activities
- Quantum Dots Synthesis And Properties
- Polysaccharides and Plant Cell Walls
- Galectins and Cancer Biology
- Advanced Chemical Sensor Technologies
- CRISPR and Genetic Engineering
- Redox biology and oxidative stress
- Molecular Biology Techniques and Applications
Zhejiang A & F University
2014-2025
Air Force Medical University
2021
Xijing Hospital
2021
National Clinical Research Center for Digestive Diseases
2021
Zhejiang University
2008-2016
Beijing National Laboratory for Molecular Sciences
2006-2015
Chinese Academy of Sciences
2006-2015
Institute of Chemistry
1996-2014
Agriculture and Forestry University
2013
ZheJiang Academy of Agricultural Sciences
2008-2012
In bacteria, OxyR is a peroxide sensor and transcription regulator, which can sense the presence of reactive oxygen species induce antioxidant system. When cells are exposed to H2O2, protein activated via formation disulfide bond between two conserved cysteine residues (C199 C208). Deinococcus radiodurans, previously unreported special characteristic DrOxyR (DR0615) found with only one cysteine. dr0615 gene mutant hypersensitive but little ionizing radiation. Site-directed mutagenesis...
A novel cyanine dye supramolecular assembly was designed to recognize mixed G-quadruplex in human telomeres.
Summary Two‐component systems are predominant signal transduction pathways in prokaryotes, and also exist many archaea as well some eukaryotes. A typical TCS consists of a histidine kinase cognate response regulator. In this study, novel gene encoding regulator (we designate it drRRA ) is identified to be essential for the extreme radioresistance Deinococcus radiodurans . DrRRA null mutant MR) sensitive gamma‐radiation compared with wild‐type strain. Transcriptional assays show that numerous...
Preliminary findings indicate that PprI is a regulatory protein stimulates transcription and translation of recA other DNA repair genes in response to damage the extremely radioresistant bacterium Deinococcus radiodurans. To define repertoire proteins regulated by investigate vivo mechanism gamma radiation, we performed comparative proteomics analyses on wild type (R1) pprI knock-out strain (YR1) under conditions ionizing irradiation. Results two-dimensional electrophoresis MALDI-TOF MS or...
The supramolecular assembly of a novel cyanine dye, 3,3′-di(3-sulfopropyl)-4,5,4′,5′-dibenzo-9-ethyl-thiacarbocyanine triethylammonium salt (ETC) was designed to verify specific intramolecular G-quadruplexes from duplex and single-strand DNAs. Spectral results have shown that ETC presented two major distinct signatures with in vitro: (i) dramatic changes the absorption spectra (including disappearance peak around 660 nm appearance independent new 584 nm); (ii) ∼70 times enhancement...
Sasa argenteastriatus, with abundant active compounds and high antioxidant activity in leaves, is a new leafy bamboo grove suitable for exploitation. To utilize it more effectively scientifically, we investigate the seasonal variations of composition its leaves activity. The argenteastriatus were collected on 5th day each month three same-sized sample plots from May 2009 to 2011. total flavonoids (TF): phenolics (TP) triterpenoid (TT) extracted contents analyzed by UV-spectrophotometer. Our...
A G-quadruplex is identified which can exhibit two different motifs, respectively, corresponding to Na+ and K+. The relative amount of each motif related the Na+/K+ ratio. Based on selective recognition motifs by a cyanine dye aggregate, method for both colorimetric quantitative measurements ratios constructed.
Abstract Background Deinococcus radiodurans accumulates high levels of manganese ions, and this is believed to be correlated with the radiation resistance ability microorganism. However, maintenance ion homeostasis in D. remains investigated. Results In study, we identified efflux protein (MntE) . The null mutant mntE was more sensitive than wild-type strain growth delayed manganese-supplemented media. Furthermore, there a substantial increase vivo concentration ions. Consistent these...
A colorimetric and fluorometric dual-modal supramolecular chemosensor has been fabricated by using the H- J-aggregates of a cyanine dye, which successfully applied to detect human serum albumin (HSA) in urine with high specificity.
Genipin has attracted much attention for its hepatoprotective, anti-inflammatory, and neuroprotection activities. However, poor water solubility active chemical properties limit application in food pharmaceutical industries. This article aimed to develop a lipid-based microemulsion delivery system improve the stability bioavailability of genipin. The excipients genipin (GME) preparation were screened pseudo-ternary phase diagram was established. droplet size (DS), zeta potential (ZP),...
Double-minded supramolecules: The interactions between human serum albumin (HSA) and J-aggregation of a cyanine dye (PTC) in phosphate buffer solution are studied. In the presence HSA, achiral J-aggregate shows chirality. chirality can be inversed by adjusting ratio HSA to PTC pH value (see picture; CD=circular dichroism). Supporting information for this article is available on WWW under http://www.wiley-vch.de/contents/jc_2267/2007/z600548_s.pdf or from author. Please note: publisher not...
Weeding out ligands: Rapid screening of G-quadruplex ligands in plant extracts is achieved by the combination recognition (see picture) with 1H NMR spectroscopy, diffusion-ordered spectroscopy (DOSY), and 2D techniques. The method has a low detection limit allows structures to be identified.
Here we present direct evidence for the vital role of RecO in Deinococcus radiodurans's radioresistance. A recO null mutant was constructed using a deletion replacement method. The exhibited growth defect and extreme sensitivity to irradiation with gamma rays UV light. These results suggest that DNA repair this organism occurs mainly via RecF pathway.