A5045752816- Glycosylation and Glycoproteins Research
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Cellular transport and secretion
- Galectins and Cancer Biology
- Heavy Metal Exposure and Toxicity
- Calcium signaling and nucleotide metabolism
- Biomedical Research and Pathophysiology
- Aquaculture disease management and microbiota
- Adenosine and Purinergic Signaling
- Effects and risks of endocrine disrupting chemicals
- Calpain Protease Function and Regulation
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Erythrocyte Function and Pathophysiology
- Folate and B Vitamins Research
- Enzyme Structure and Function
- Essential Oils and Antimicrobial Activity
- Genomic variations and chromosomal abnormalities
- CRISPR and Genetic Engineering
- Rice Cultivation and Yield Improvement
- Ionic liquids properties and applications
- Lipid metabolism and disorders
- Zebrafish Biomedical Research Applications
- Ubiquitin and proteasome pathways
University of Brescia
2005-2023
University of Milano-Bicocca
2021
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2021
Universitat de Barcelona
2004
Telethon Institute Of Genetics And Medicine
1999-2001
Telethon Foundation
2001
Vitenparken
2000
Several mammalian sialidases have been described so far, suggesting the existence of numerous polypeptides with different tissue distributions, subcellular localizations and substrate specificities. Among these enzymes, plasma-membrane-associated sialidase(s) a pivotal role in modulating ganglioside content lipid bilayer, their involvement complex mechanisms governing cell-surface biological functions. Here we describe identification expression human sialidase, NEU3, isolated starting from...
Cystinuria (OMIM 220100) is a common recessive disorder of renal reabsorption cystine and dibasic amino acids that results in nephrolithiasis cystine. Mutations SLC3A1, which encodes rBAT, cause Type I cystinuria, mutations SLC7A9, putative subunit rBAT (bo,+AT), non-Type cystinuria. Here we describe the genomic structure SLC7A9 (13 exons) 28 new this gene that, together with seven previously reported, explain 79% alleles 61 cystinuria patients. These data demonstrate main gene. G105R,...
Several mammalian sialidases have been described so far, suggesting the existence of numerous polypeptides with different tissue distributions, subcellular localizations and substrate specificities. Among these enzymes, plasma-membrane-associated sialidase(s) a pivotal role in modulating ganglioside content lipid bilayer, their involvement complex mechanisms governing cell-surface biological functions. Here we describe identification expression human sialidase, NEU3, isolated starting from...
Sialidase NEU3 is also known as the plasma-membrane-associated form of mammalian sialidases, exhibiting a high substrate specificity towards gangliosides. In this respect, sialidase modulates cell-surface biological events and plays pivotal role in different cellular processes, including cell adhesion, recognition differentiation. At moment, no detailed studies concerning subcellular localization are available, mechanism its association with membranes still unknown. present study, we have...
Mucolipin‐1 is a 65‐kDa membrane protein encoded by the MCOLN1 gene, which mutated in patients with mucolipidosis type IV (MLIV), rare neurodegenerative lysosomal storage disorder. We studied subcellular localization of wild‐type and three different mutant forms (T232P, F408del F465L) mucolipin expressing Myc‐tagged proteins HeLa cells. The overexpressed colocalizes to late endocytic structures induces an aberrant distribution these compartments. F465L MLIV show similar protein, whereas...
Sialidases remove sialic acid residues from various sialo-derivatives. To gain further insights into the biological roles of sialidases in vertebrates, we exploited zebrafish (Danio rerio) as an animal model. A transcriptome- and genome-wide search using sequences human NEU polypeptides templates revealed presence seven different genes related to sialidases. neu1 neu4 are putative orthologues mammalian NEU1 NEU4 respectively. Interestingly, remaining organized clusters located on chromosome...
Transposable elements, as major components of most eukaryotic organisms' genomes, define their structural organization and plasticity. They supply host genomes with functional for example, binding sites the pleiotropic master transcription factor p53 were identified in LINE1, Alu LTR repeats human genome. Similarly, this report we reveal role zebrafish (Danio rerio) EnSpmN6_DR non-autonomous DNA transposon shaping repertoire target genes. The multiple copies embedded responsive elements...
Abstract To orchestrate the genomic response to cellular stress signals, p53 recognizes and binds DNA containing specific well-characterized p53-responsive elements (REs). Differences in RE sequences can strongly affect transactivation capacity occur even between closely related species. Therefore, identification characterization of a species-specific Binding sistes (BS) consensus sequence associated target genes may help provide new insights into evolution regulatory networks across...
Sialic acid acetylesterase (SIAE) removes acetyl moieties from the carbon 9 and 4 hydroxyl groups of sialic recently a debate has been opened on its association to autoimmunity. Trying get new insights this intriguing enzyme we have studied siae in zebrafish (Danio rerio). In teleost encodes for polypeptide with high degree sequence identity human mouse counterparts. Zebrafish Siae behavior upon transient expression COS7 cells is comparable concerning pH optimum activity, subcellular...
Essential oils are widely recognized as natural alternatives to pharmaceutical antibacterial and antifungal agents. With respect standard pharmaceutics, the advantages of essential their (i) low production costs, (ii) lack chemical biochemical drawbacks that intrinsic synthetic process (iii) good tolerance by humans. On other hand, liquid nature poses concerns about actual application in different therapeutic issues regarding persistence ability control or prolong drug release. In this...
In this paper, we report the metabolic characterization of two foci, F1 and F3, obtained at end Cell Transformation Assay (CTA), performed by treating C3H10T1/2Cl8 mouse embryo fibroblasts with 1 μM CdCl2 for 24 h. The elucidation cadmium action mechanism can be useful both to improve in vitro CTA yield insights into carcinogenesis. metabolism foci was investigated through Seahorse enzyme activity assays; mitochondria were studied confocal microscopy reactive oxygen species detected flow...
Zebrafish encodes several sialidases belonging to the NEU3 group, plasma membrane-associated member of family with high specificity toward ganglioside substrates. Neu3.1, Neu3.2 and Neu 3.3 have been expressed in E. coli purified using pGEX-2T expression system. Although all enzymes are by bacterial cells, Neu3.1 formed insoluble aggregates that hampered its purification. Neu3.3 oligomers as demonstrated gel filtration chromatography experiments. Actually, first a trimer whereas second...
<title>Abstract</title> In this paper we report the metabolic characterization of two <italic>foci</italic>, F1 and F3, obtained at end Cell Transformation Assay (CTA), performed by treating C3H10T1/2Cl8 mouse embryo fibroblasts with 1 µM CdCl<sub>2</sub> for 24 h. The elucidation cadmium action mechanism can be useful both to improve <italic>in vitro</italic> CTA yield insights into carcinogenesis. We previously showed that, despite being completely transformed type III F3...
Sialidases remove terminal sialic acids residues from the non-reducing ends of glycoconjugates. They have been recognized as catabolic enzymes that work within different subcellular compartments and can ensure proper turn-over Four mammalian sialidases (NEU1-4) exist, with localization, pH optimum substrate specificity. In zebrafish, seven sialidases, high homology to counterparts, identified. Zebrafish Neu3.2 is similar human cytosolic sialidase NEU2, which involved in skeletal muscle...