A5046348705- Silicon Nanostructures and Photoluminescence
- Biochemical effects in animals
- Biochemical Acid Research Studies
- Neuroscience and Neural Engineering
- Neurological diseases and metabolism
- Enzyme Structure and Function
- RNA Interference and Gene Delivery
- Anodic Oxide Films and Nanostructures
- Semiconductor materials and devices
- Mesoporous Materials and Catalysis
- Nanowire Synthesis and Applications
- Nerve injury and regeneration
- Electrochemical sensors and biosensors
University of Bergen
2016-2021
A porous Si (pSi) microparticle-based delivery system is investigated, and the intrinsic luminescence from particles employed as a probe to monitor release of model protein payload, bovine serum albumin (BSA). The microparticles consist core skeleton surrounded by SiO2 shell. Two types pSi are tested, one with smaller (10 nm) pores other larger (20 pores. pore material yields higher mass loading BSA (3 vs 20%). different methods used load into these nanostructures: first involves...
Abstract Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in biosynthesis of catecholamine neurotransmitters. TH is a highly complex enzyme at mechanistic, structural and regulatory levels preparation kinetically conformationally stable for characterization has been challenging. Here, we report on improved protocols purification recombinant human isoform 1 (TH1), which provide large amounts pure, stable, active TH1 with an intact N-terminus. purified through fusion His-tagged...
Enzyme replacement therapy (ERT) is a therapeutic approach envisioned decades ago for the correction of genetic disorders, but ERT has been less successful disorders with neurological manifestations. In this work, we have tested functionality nanoparticles (NP) composed maltodextrin lipid core to bind and stabilize tyrosine hydroxylase (TH). This complex unstable brain enzyme that catalyzes rate-limiting step in synthesis dopamine other catecholamine neurotransmitters. We characterized these...
Tyrosine hydroxylase (TH) is the enzyme catalyzing rate-limiting step in synthesis of dopamine brain. Developing replacement therapies using TH could therefore be beneficial to patient groups with deficiency, and use nanocarriers that cross blood–brain barrier seems advantageous for this purpose. Nanocarriers may also help maintain structure function TH, which complex unstable. Understanding how interact a nanocarrier crucial investigation such therapeutic applications. This work describes...