A5004449572- Bacteriophages and microbial interactions
- Chromatin Remodeling and Cancer
- Neurofibromatosis and Schwannoma Cases
- Genomics and Phylogenetic Studies
- Cytokine Signaling Pathways and Interactions
- Microbial Natural Products and Biosynthesis
- Plant Reproductive Biology
- Genetics and Neurodevelopmental Disorders
- Chromosomal and Genetic Variations
- Microbial infections and disease research
- Axon Guidance and Neuronal Signaling
- Glioma Diagnosis and Treatment
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- Plant Virus Research Studies
- Neuroblastoma Research and Treatments
Indiana University
2025
University School
2025
Indiana University School of Medicine
2025
Washington University in St. Louis
2019-2023
Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder caused by spectrum of distinct germline NF1 gene mutations, traditionally viewed as equivalent loss-of-function alleles. To specifically address the issue mutational equivalency in disease with considerable clinical heterogeneity, we engineered seven isogenic human induced pluripotent stem cell lines, each different patient mutation, to identify potential differential effects mutations on central nervous system cells and...
Neurodevelopmental disorders are often caused by chromosomal microdeletions comprising numerous contiguous genes. A subset of neurofibromatosis type 1 (NF1) patients with severe developmental delays and intellectual disability harbors such a microdeletion event on chromosome 17q11.2, involving the NF1 gene flanking regions (NF1 total deletion [NF1-TGD]). Using patient-derived human induced pluripotent stem cell (hiPSC)-forebrain cerebral organoids (hCOs), we identify both neural (NSC)...
Single-nucleus RNA sequencing (snRNA-seq) enables resolving cellular heterogeneity in complex tissues. snRNA-seq overcomes limitations of traditional single-cell RNA-seq by using nuclei instead cells, allowing to utilize frozen tissues and difficult-to-isolate cell types. Although various isolation methods have been developed, systematic evaluations their effects on nuclear integrity subsequent data quality remain lacking, a critical gap with profound implications for the rigor...
Abstract Single nucleotide variants in the general population are common genomic alterations, where majority presumed to be silent polymorphisms without known clinical significance. Using human induced pluripotent stem cell (hiPSC) cerebral organoid modeling of 1.4 megabase Neurofibromatosis type 1 (NF1) deletion syndrome, we previously discovered that cytokine receptor-like factor-3 (CRLF3) gene, which is co-deleted with NF1 functions as a major regulator neuronal maturation. Moreover,...
We present here the complete genomes of two Streptomyces bacteriophages, Satis and JustBecause. Both phages were isolated directly from soil samples collected in St. Louis, MO, with an unusual prolate head morphology large genome lengths over 180 kb.