Chinese hamster ovary (CHO) cells are the primary platform for production of biopharmaceuticals. To increase yields, many CHO cell lines have been genetically engineered to resist death. However, kinetics that governs fate in bioreactors confounded by variables associated with batch processes. Here, we used CRISPR-Cas9 create combinatorial knockouts three known BCL-2 family effector proteins: Bak1, Bax, and Bok. assess response apoptotic stimuli, were cultured presence four cytotoxic...

TDCS for photo-double ionization of helium have been measured in a PhotoElectron-PhotoElectron COincidence (PEPECO) experiment. The obtained the first time at very low excess energies E, 0.6 eV<E<2 eV, both equal and unequal energy sharing between two outgoing electrons. data are compared with predictions Wannier model, also recent, non-Wannier, ab initio calculations. In addition, time, relative magnitudes various determined this region, suggesting departure from model largest studied, E=2 eV.

The radiative decay of $^{1}P^{o}$ doubly excited states in helium has been investigated using a novel apparatus which metastable atoms and vacuum ultraviolet photons are detected. intensity ratio the energetically narrow $(sp,2n\ensuremath{-})^{1}P^{o}$ $(2p,nd)^{1}P^{o}$ series to broader $(sp,2n+)^{1}P^{o}$ is strikingly enhanced comparison with conventional photoabsorption, photoion, or photoelectron measurements synchrotron radiation. experimental approach new way forward for study states.

The authors have studied near-threshold photoionisation of argon in the region valence satellites (32 to 49 eV) two ways. They measured photoelectron spectra for various constant values energy, from zero 0.48 eV. Secondly, they relative partial ionisation cross sections many ionic states this region, over energy range 1.0 eV above their respective thresholds. dominant contribution these is observed come decay doubly excited neutral or resonances.

Doubly excited neutral states of argon have been investigated by observing their decay into the 3p and 3s main ionisation lines satellite ionic states. These were measured using photoelectron spectroscopy monochromatic synchrotron radiation in range 31.0 to 42.3 eV. The line constant state spectra show structure due doubly but general these are much less prominent than for satellites which dominated resonances, particularly close threshold. There is also a strong selectivity with marked...

A transmission W/B(4)C multilayer has been designed and characterized which shows significant phase retardation up to a photon energy of 1 keV, when operated near the Bragg condition. This allows, for first time, full polarization vector soft x-radiation be measured keV in self-calibrating method. Quantitative polarimetry is now possible across 2p edges all transition metals.

Autoionization spectra of CH2Cl2 and CD2Cl2 molecules after Cl 2p excitation are studied. The two molecular atomic Auger transitions examined assigned. contribution is lower in the deuterated molecule. In addition, to support presence ultrafast dissociation mechanism dichloromethane molecule, a series high-level ab initio quantum mechanical calculations were performed at multiconfigurational self-consistent field (MCSCF) multireference configuration interaction (MRCI) levels theory. Minimum...

A new type of photoelectron/photoion coincidence spectrometer using a penetrating field technique has been used to investigate photo-double ionization processes in helium. Threshold measurements have determine the value exponent Wannier theory and its range validity. The obtained is consistent with prediction. Photoelectron/photoion also behaviour asymmetry parameter, beta , for near threshold constant close -0.4, observed. This result disagreement predictions which appears underestimate...

The pyridine molecule has been examined by the means of photoelectron and threshold spectroscopies. Ionization energies were determined for both outer inner valence orbitals new adiabatic values also resolved. Vibronic structure associated with several states was assigned mainly to be due C–C stretches ring bends. Additionally a Rydberg state converging 7b2 ascribed. data shown here are in good agreement previous results brings some insights into electronic this biologically astrochemically...

Soft X-rays (90-173 eV) from the 3rd generation Canadian Light Source have been used in conjunction with a multi coincidence time and position sensitive detection apparatus to observe dissociative ionization of OCS. By varying X-ray energy we can compare dynamics direct Auger processes, access channels which result two or three body breakup, 2+ 4+ states. We make several new observations for 3+ state such as kinetic release limited by photon energy, using Dalitz plots see evidence timescale...

Changes in milk yield and composition from mature Holstein-Friesian cows managed under low fluctuating environmental temperatures have been presented. The experiment conducted at the University of Saskatchewan, Saskatoon, lasted for 106 days during winter 1956–57. Loafing barn temperatures, measured continuously degree-hours per day (d-h/day) ranged 110 to 1202 daily minimum ambient air temperature (DMAAT) varied −5° 38°F.As decreased below 25°F., significantly. Milk was unaffected by...

Abstract Both calcite and vaterite polyerystalline phases of LuBO3 doped with cerium praseodymium have been prepared their fluorescence properties investigated in the visible, near UV VUV regions. The systems exhibit a strong rapid UV-blue typical 5d → 4f parity-allowed transitions dopants. It is shown that excitation transfers from excitions electron-hole pairs to dopants are very efficient. light yield can be three times more efficient than well-known Bi4Ge3O12 scintillator for best samples.

A W: B4C multilayer phase retarder has been designed and characterized which shows a nearly constant retardance between 640 850 eV photon energies when operated near the Bragg condition. This freestanding transmission was used successfully to determine, for first time, full polarization vector at soft x-ray above 600 eV, not possible before due lack of suitable optical elements. Thus, quantitative polarimetry is now 2p edges magnetic substances Fe, Co, Ni benefit circular dichroism...

The reliable and cost-efficient manufacturing of monoclonal antibodies (mAbs) is essential to fulfil their ever-growing demand. Cell death in bioreactors reduces productivity product quality, largely attributed apoptosis. In perfusion bioreactors, this leads the necessity a bleed stream, which negatively affects overall process economy. To combat limitation, death-resistant Chinese hamster ovary cell lines were developed by simultaneously knocking out apoptosis effector proteins Bak1, Bax,...

We investigate the polarization changes simultaneously occurring with intensity due to carbon contamination of optical components at two plane grating beam lines PGM-1 and PGM-2 BESSY II. The are very similar each other connect same APPLE II type undulator UE56/2. spectra measured initial horizontal vertical differ for lines. For polarimetry measurements 8-axis polarimeter is equipped a Cr/Sc multilayer in transmission as phase retarder reflecting operating near Brewster angle analyzer....

Calculations based on the theory of radiationless resonant Raman scattering are employed in interpretation new $\mathrm{Xe}{L}_{3}{\ensuremath{-}M}_{4,5}{M}_{4,5}$ Auger spectra recorded using synchrotron radiation tuned to energies across ${L}_{3}$ edge. Fits theoretical line shapes separating intensities due $\mathrm{nd}$ spectator (resonant) and diagram processes. Near-threshold intensity, previously attributed decay, is found be large$\ensuremath{-}n$ lines that result from...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe P(4Su) + N3(2IIg) reaction: chemical generation of a new metastable state PNT. L. Henshaw, M. A. MacDonald, D. H. Stedman, and R. CoombeCite this: J. Phys. Chem. 1987, 91, 11, 2838–2842Publication Date (Print):May 1, 1987Publication History Published online1 May 2002Published inissue 1 1987https://pubs.acs.org/doi/10.1021/j100295a037https://doi.org/10.1021/j100295a037research-articleACS PublicationsRequest reuse permissionsArticle...

In this study effects of estradiol and testosterone injections thyroidectomy on muscle fibre diameters ewe wether yearlings were determined. Forty-eight sheep, half which ewes wethers, subdivided into estradiol, testosterone, control groups containing equal numbers each sex. Half the animals within sex group treatment thyroidectomized. Biweekly rates intramuscular injection 5 mg. 175 mg., respectively. All received same amount feed per day throughout a 195-day experimental period.Live weight...

The asymmetry parameters for photo-double ionization of helium up to 2 eV above threshold have been measured using a coincidence photoelectron spectrometer and synchrotron radiation. observations were made the extreme case unequal energy partitioning between two photoelectrons wherein one electron has near-zero other carries off all excess energy. measurements show that, even in this special case, correlation still plays dominant role.

We investigate polarisation properties of a reflective Mo/Si multilayer system in the EUV range using polarized synchrotron radiation at BESSY-II. The characterization involves reflectivity measurements with s- and p-polarized light as function wavelength for three different angles near normal incidence. phase retardance is determined incidence one fixed angle wavelength. As an additional spin-off polarimetry measurement Stokes parameters beamline could be determined. With 8-axis...