Electronic state spectroscopy of limonene has been investigated using vacuum ultraviolet photoabsorption in the energy range 5.0–10.8 eV. The availability a high resolution photon beam (∼0.075 nm) enabled detailed analysis vibrational progressions and allowed us to propose, for first time, new assignments several Rydberg series. Excited states located 7.5–8.4 eV region have studied time. A He(I) photoelectron spectrum also recorded from 8.2 9.5 compared previous low works. value 8.521 ±...

In order to determine a proper value for the density of dry DNA films we have used method based upon measurement interference effects in transmission spectra thin layers. Our results show that methodology is effective and this state, 1.407 g/cm(3), much lower than commonly 1.7 g/cm(3). Obtaining accurate values film will allow optical constants be recalculated, which were previously obtained assuming higher density. Furthermore, since our recent investigations shown strong dependence sample...

The pyridine molecule has been examined by the means of photoelectron and threshold spectroscopies. Ionization energies were determined for both outer inner valence orbitals new adiabatic values also resolved. Vibronic structure associated with several states was assigned mainly to be due C–C stretches ring bends. Additionally a Rydberg state converging 7b2 ascribed. data shown here are in good agreement previous results brings some insights into electronic this biologically astrochemically...

To accurately quantify the number of single-strand breaks (SSBs) induced in plasmid DNA molecules after irradiation, a new type assay methodology has been explored. The method is based on TUNEL (terminal deoxynucleotide transferase dUTP nick end-labeling) that was adopted for use under ELISA (enzyme-linked immunosorbent assay) conditions. found to both improve quantification and reduce uncertainties measurement SSBs compared with commonly used agarose gel electrophoresis (AGE) method....

The highest resolution vacuum ultraviolet photoabsorption spectrum of ethyl formate, C2H5OCHO, yet reported is presented over the wavelength range 115.0–275.5 nm (10.75–4.5 eV) revealing several new spectral features. Valence and Rydberg transitions their associated vibronic series, observed in spectrum, have been assigned accordance with ab initio calculations vertical excitation energies oscillator strengths. Calculations also carried out to determine ionization fine structure lowest ionic...

The high-resolution vacuum ultraviolet photoabsorption spectrum of ethyl acetate, C4H8O2, is presented over the energy range 4.5−10.7 eV (275.5−116.0 nm). Valence and Rydberg transitions their associated vibronic series observed in spectrum, have been assigned accordance with new ab initio calculations vertical excitation energies oscillator strengths. Also, cross sections used to calculate photolysis lifetime this ester upper stratosphere (20−50 km). Calculations also carried out determine...

2-Amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) and ethylenediaminetetraacetic acid (EDTA) are key components of biological buffers frequently used as DNA stabilizers in irradiation studies. Such surface or liquid phase studies done with the aim to understand fundamental mechanisms radiation damage improve cancer radiotherapy. When ionizing is used, abundant secondary electrons formed during process, which able attach molecular compounds present on surface. In study we experimentally...

Laser illuminated gold nanoparticles (AuNPs) efficiently absorb light and heat up the surrounding medium, leading to versatile applications ranging from plasmonic catalysis cancer photothermal therapy. Therefore, an in-depth understanding of thermal, optical, electron induced reaction pathways is required. Here, electrophilic DNA nucleobase analog 5-Bromouracil (BrU) has been used as a model compound study its decomposition in vicinity AuNPs with intense ns laser pulses under various...

Irradiations of plasmid DNA by both X-rays and UV light in the presence absence compounds containing platinum bromine atoms were performed order to asses sensitization potential these compounds. Plasmid pBR322 was incubated with (II) bromide, hydrogen hexabromoplatinate (IV), hexahydroxyplatinate (IV) sodium (IV). Incubation followed X-ray or irradiations. It found that amongst sensitizers tested, during irradiations carried out highest levels double strand breaks formation upon treatment...

Dissociative electron attachment to hydroxyurea was studied in the gas phase for energies ranging from zero 9 eV order probe its radiosensitizing capabilities. The experiments were carried out using a hemispherical monochromator coupled with quadrupole mass spectrometer. Diversified fragmentation of observed upon low energy and here we highlight major dissociation channels. Moreover, thermodynamic thresholds various reactions are reported support discussion experimental findings. dominant...

We present the experimental high-resolution vacuum ultraviolet (VUV) photoabsorption spectra of phenol covering for first time full 4.3–10.8 eV energy-range, with absolute cross sections determined. Theoretical calculations on vertical excitation energies and oscillator strengths were performed using time-dependent density functional theory equation-of-motion coupled cluster method restricted to single double excitations level. These have been used in assignment valence Rydberg transitions...

Two nanometre gold nanoparticles (AuNPs), bearing sugar moieties and/or thiol-polyethylene glycol-amine (PEG-amine), were synthesised and evaluated for their in vitro toxicity ability to radiosensitise cells with 220 kV 6 MV X-rays, using four cell lines representing normal cancerous skin breast tissues. Acute 3 h exposure of AuNPs, PEG-amine only or a 50:50 ratio alpha-galactose derivative resulted selective uptake towards cancer at unprecedentedly low nanomolar concentrations....

X-ray irradiation of plasmid DNA in presence platinum (II)-based compounds was carried out order to assess the radiosensitization capabilities these drugs. In present investigations pBR322 used monitor effectiveness chosen inducing strand breaks. Samples were incubated potential radiosensitisers: (II) bromide and cis-diamminedibromoplatinum (II). The results examined against a common cancer chemotherapy drug cis-diamminedichloroplatinum It found that can greatly increase levels single-...

The highest resolution vacuum ultraviolet photoabsorption spectrum of isobutyl formate, C5H10O2, yet reported is presented over the energy range 4.5-10.7 eV (275.5-118.0 nm) revealing several new spectral features. Valence and Rydberg transitions their associated vibronic series observed in have been assigned accordance with ab initio calculations vertical excitation energies oscillator strengths. Calculations also carried out to determine ionization fine structure lowest ionic state formate...

Interactions of VUV light and DNA samples in aqueous solutions are reported. The damage induced by such radiation is quantified monitoring both loss supercoiled formation single double strand breaks using agarose gel electrophoresis. Irradiations were performed synchrotron photons 130, 150, 170 190 nm. irradiation experiments revealed enhanced upon with nm as compared irradiations 150 130 carried at caused the least damage.

This paper describes some insights on applicability of a Selective Laser Melting and Direct Metal Sintering technology-manufactured turbine blade models for aerodynamic tests in wind tunnel. The principal idea behind this research was to assess the possibilities using 'raw' DLMS printed gas-flow experiments. actual blade, manufactured technology, is assessed terms surface quality (roughness), geometrical shape size (outline), counterbores small diameter holes. results are evaluated...

We investigate the methanol absorption spectrum in range 5.5-10.8 eV to provide accurate and absolute cross-sections values. The main goal of this study is a comprehensive analysis electronic-state spectroscopy by employing high-resolution vacuum ultraviolet (VUV) photoabsorption measurements together with state-of-the-art quantum chemical calculation methods. VUV reveals several new features that are not previously reported literature, for n > 3 transitions (nsσ(a') ← (2a″)) (1A' X̃1A')...