Previous studies suggested that PspC is important in adherence and colonization within the nasopharynx. In this study, we conducted mutational to further identify role plays pathogenesis of pneumococci. pspC and/or pspA was insertionally inactivated a serotype 2 Streptococcus pneumoniae strain 19 S. strain. mouse model, pneumococcal strains with mutations were significantly attenuated their abilities colonize. pneumonia unable infect or multiply lung. Using reverse transcriptase PCR able...

ABSTRACT It was previously proposed that autolysin's primary role in the virulence of pneumococci to release pneumolysin an extracellular location. This interpretation came into question when observed be released significant amounts from some during log-phase growth, because autolysis not believed occur at this time. We have reexamined phenomenon detail for one such strain, WU2. study found WU2 dependent on autolysin action. A mutant lacking showed same pattern as wild-type strain. Addition...

ExoT is a type III secreted effector protein found in almost all strains of Pseudomonas aeruginosa and required for full virulence an animal model acute pneumonia. It comprised N-terminal domain with GTPase activating (GAP) activity towards Rho family GTPases C-terminal ADP ribosyltransferase (ADPRT) minimal synthetic substrate vitro. Consistent its as GTPase, has been shown to inhibit P. internalization into epithelial cells macrophages, disrupt the actin cytoskeleton through Rho-dependent...

Pseudomonas aeruginosa, an important cause of opportunistic infections in humans, delivers bacterial cytotoxins by type III secretion directly into the host cell cytoplasm, resulting disruption signaling and innate immunity. However, little is known about fate toxins themselves following injection cytosol. Here, we show both vitro vivo studies that ubiquitin ligase Cbl-b interacts with III-secreted effector exotoxin T (ExoT) plays a key role limiting dissemination mediated ExoT. We...

The Helicobacter pylori CagA protein induces profound morphological changes in the host cytoskeleton and cell scattering, but signalling involved is poorly understood. Pseudomonas aeruginosa also affects actin a variety of ways by injecting ExoS ExoT toxins which encode N-terminal GTPase activating C-terminal ADP-ribosyltransferase (ADPRT) activities. In this study we developed novel coinfection assay to gain new insights into effector functions. We found that P. either or efficiently...

Abstract Real-time PCR methods for detecting foodborne pathogens offer the advantages of simplicity and quick time-to-results compared to traditional culture methods. In this study, MicroSEQ® real-time system was evaluated detection Salmonella spp. in 10 different food matrixes following AOAC Research Institute's Performance Tested MethodSM validation program. addition, performance MicroSEQ peanut butter as a part Emergency Response Validation Program sponsored by Institute. The ISO 6579...

Abstract Modern molecular methods offer the advantages of simplicity and short time-to-results compared to traditional culture methods. We describe validation a new Real-Time PCR method detect E. coli O157:H7 in five food matrixes. The complete system consists MicroSEQ®E. Detection Kit, sample preparation (two methods, PrepSEQ® Nucleic Acid Extraction Kit PrepSEQ Rapid Spin Sample Preparation were validated), Applied Biosystems 7500 Fast instrument, RapidFinder™ Express software. test was...

A complete system for real-time PCR detection of Listeria species was validated in five food matrixes and environmental surfaces, namely, hot dogs, roast beef, lox (smoked salmon), pasteurized whole cow's milk, dry infant formula, stainless steel, plastic cutting board, ceramic tile, rubber sheets, sealed concrete. The consists the MicroSEQ spp. Detection Kit, two sample preparation kits (PrepSEQ Nucleic Acid Extraction Kit PrepSEQ Rapid Spin Sample Preparation Kit), Applied Biosystems 7500...

Increasingly, more food companies are relying on molecular methods, such as PCR, for pathogen detection due to their improved simplicity, sensitivity, and rapid time results. This report describes the validation of a new Real-Time PCR method detect Listeria monocytogenes in nine different matrixes. The complete system consists MicroSEQ L. Detection Kit, sample preparation, Applied Biosystems 7500 Fast instrument, RapidFinder Express software. Two preparation methods were validated: PrepSEQ...

Peanut butter spiked with Salmonella enterica ser. Typhimurium was prepared by an independent laboratory and sent to Applied Biosystems determine the sensitivity specificity of TaqMan Detection Kit for detecting in peanut butter. The samples were at three levels: five no-spike (0 CFU/25 g); 20 low-spike (0.2 high-spike (2 g). They coded create a blind set 45 samples. processed based on unpaired test design that included enrichment buffered peptone water candidate method lactose broth...

Abstract Increasingly, more food companies are relying on molecular methods, such as PCR, for pathogen detection due to their improved simplicity, sensitivity, and rapid time results. this report describes the validation of a new Real-time PCR method detect Listeria monocytogenes in nine different matrixes. complete system consists MicroSEQ®L. Detection Kit, sample preparation, Applied Biosystems 7500 Fast instrument, RapidFinder™ Express software. two preparation methods were validated:...

Abstract The Applied Biosystems Performance Tested MethodSM for detecting Listeria monocytogenes in food and environmental samples was compared to the Health Canada reference method (MFHPB-30) analysis of five ready-to-eat (RTE) meats (deli turkey, hot dogs, liver paté, deli ham, raw fermented sausage) a stainless steel surface. MicroSEQ® includes MicroSEQListeria Detection Kit option two different sample preparation kits, either automated high-throughput PrepSEQ™ Nucleic Acid Extraction or...

SUMMARY Healthcare linens act as a vector of microbial transmission through use, storage and handling. In this retrospective multi-year, multi-site study, the impact an infection prevention program, that included automated silver ion-based antimicrobial laundry treatment, was studied. A composite reduction 42% in healthcare associated (HAI) rates observed, with biggest reductions CAUTI CDI rates. Although further study is needed to better understand exact contribution such intervention...

Abstract The Applied Biosystems Performance Tested MethodSM for detecting Listeria species in food and environmental samples was compared to the Health Canada reference method (MFHPB-30) analysis of five ready-to-eat (RTE) meats (deli turkey, hot dogs, liver paté, deli ham, raw fermented sausage) a stainless steel surface. MicroSEQ includes MicroSEQ®Listeria spp. Detection Kit option two different sample preparation kits, either automated high-throughput PrepSEQ™ Nucleic Acid Extraction or...

Community-acquired infections caused by Staphylococcus and MRSA can spread easily through sharing towels, gear contaminated surfaces. The resulting skin lead to athlete disqualifications, cancellations of competitions potential impact on team performance. In this study, we evaluate a residual antimicrobial textile treatment as an environmental hygiene infection control strategy improved cleanliness reduced risk for infection. PURPOSE: To determine the silver-based levels athletic gear,...

Community-acquired infections caused by Staphylococcus and MRSA can spread easily through sharing towels, gear contaminated surfaces. The resulting skin lead to athlete disqualifications, cancellations of competitions potential impact on team performance. In this study, we evaluate a residual antimicrobial textile treatment as an environmental hygiene infection control strategy improved cleanliness reduced risk for infection. PURPOSE: To determine the silver-based levels athletic gear,...