A5087483514- Mosquito-borne diseases and control
- Plant Virus Research Studies
- T-cell and B-cell Immunology
- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Escherichia coli research studies
- Insect Resistance and Genetics
- Virology and Viral Diseases
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Bacteriophages and microbial interactions
Stony Brook University
2015-2021
Stony Brook School
2021
Significance Arboviruses (arthropod-borne viruses), a large group of RNA viruses, replicate in insects that transmit them to mammals, their second host. Insects and mammals have evolved different protein encoding strategies (codon pair bias); hence, arboviruses must delicately balance encodings between two phyla. Using dengue virus (DENV), the most important human arbovirus pathogen, as model, we have, by computer design chemical synthesis, undone this codon bias favor insects. Recoded DENVs...
Computer design and chemical synthesis generated viable variants of poliovirus type 1 (PV1), whose ORF (6,189 nucleotides) carried up to 1,297 "Max" mutations (excess overrepresented synonymous codon pairs) or 2,104 "SD" (randomly scrambled codons). "Min" underrepresented are nonviable except for P2
Dengue virus (DENV), an arthropod-borne ("arbovirus") virus, causes a range of human maladies ranging from self-limiting dengue fever to the life-threatening shock syndrome and proliferates well in two different taxa Animal Kingdom, mosquitoes primates. Mosquitoes primates show taxonomic group-specific intolerance certain codon pairs when expressing their genes by translation. This is called "codon pair bias". By necessity, viruses evolved delicately balance this fundamental difference open...
The sequence of Zika virus has evolved as it spread out Africa and into the Americas. It is unclear whether American strains define a new serotype. Here, we have tested virulence immunogenicity three wild-type ZIKV in neonatal Swiss Webster mice. We found that all (African MR766, 1947; Asian FSS13025, 2010; American, PRVABC59, 2015) are capable killing mice after intracranial injection. Intraperitoneal injection with these viruses did not kill, but produced neutralizing antibodies measured...
Abstract Dengue virus (DENV), an arthropod-borne (“arbovirus”) causing a range of human maladies ranging from self-limiting dengue fever to the life-threatening shock syndrome, proliferates well in two different taxa Animal Kingdom, mosquitoes and primates. Unexpectedly, primates have distinct preferences when expressing their genes by translation, e.g. members these show taxonomic group-specific intolerance certain codon pairs. This is called “codon pair bias”. By necessity, arboviruses...
Abstract γδ T cells are critical sentinels protecting barrier surfaces against pathogens. However, our understanding of cell specificity remains limited. We previously identified a resident memory Vγ4Vδ1 population elicited by foodborne Listeria monocytogenes (Lm) infection. Analysis the CDR3δ sequences revealed that large subset non-canonical clones expanded during primary Lm infection, contracted homeostasis and recalled after secondary infection suggesting antigenic-specificity may be...
Abstract γδ T cells are pleiotropic sentinels involved in immune defense at barrier tissues. While most cell subsets associated with innate-like responses, foodborne Listeria monocytogenes (Lm) infection elicits a substantial resident memory Vγ4Vδ1 response that appears specific to the priming pathogen despite limited receptor (TCR) diversity. As our understanding of remains elusive, were examined using heterologous challenge infections coupled TCRδ sequencing reveal an unanticipated...