Léa Girard

ORCID: 0000-0002-2163-5924
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About
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Research Areas
  • Genomics and Phylogenetic Studies
  • Plant Pathogenic Bacteria Studies
  • Vibrio bacteria research studies
  • Microbial Community Ecology and Physiology
  • Microbial Natural Products and Biosynthesis
  • Plant-Microbe Interactions and Immunity
  • Bacterial biofilms and quorum sensing
  • Bacterial Genetics and Biotechnology
  • Aquaculture disease management and microbiota
  • Microbial infections and disease research
  • Mycotoxins in Agriculture and Food
  • Biochemical Analysis and Sensing Techniques
  • Antibiotic Resistance in Bacteria
  • Antimicrobial Peptides and Activities
  • Dye analysis and toxicity
  • Yersinia bacterium, plague, ectoparasites research
  • Bacterial Identification and Susceptibility Testing
  • Legionella and Acanthamoeba research
  • Erythrocyte Function and Pathophysiology
  • Biosensors and Analytical Detection
  • Legume Nitrogen Fixing Symbiosis
  • Catalysis and Hydrodesulfurization Studies
  • Catalytic Processes in Materials Science
  • RNA and protein synthesis mechanisms
  • Industrial Gas Emission Control

KU Leuven
2019-2023

Sorbonne Université
2017-2019

Centre National de la Recherche Scientifique
2017-2019

Laboratoire de Biodiversité et Biotechnologies Microbiennes
2017-2019

BC Innovation Council
1968

The genus Pseudomonas hosts an extensive genetic diversity and is one of the largest genera among Gram-negative bacteria. Type strains are well known to represent only a small fraction this number available genome sequences increasing rapidly. Consequently, new species regularly reported within constantly evolving. In study, whole sequencing enabled us define 43 provide update evolutionary taxonomic relationships. Phylogenies based on rpoD gene sequences, including, respectively, 316 313...

10.3390/microorganisms9081766 article EN cc-by Microorganisms 2021-08-18

Pseudomonas species are prominent producers of lipopeptides that support proliferation in a multitude environments and foster varied lifestyles. By genome mining biosynthetic gene clusters (BGCs) with lipopeptide-specific organization, we mapped the global lipopeptidome linked its staggering diversity to taxonomy producers, belonging different groups within major fluorescens lineage.

10.1128/msystems.00988-22 article EN cc-by mSystems 2023-01-31

The taxonomic affiliation of Pseudomonas isolates is currently assessed by using the 16S rRNA gene, MultiLocus Sequence Analysis (MLSA), or whole genome sequencing. Therefore, microbiologists are facing an arduous choice, either universal marker, knowing that these affiliations could be inaccurate, engaging in more laborious and costly approaches. rpoD like included most MLSA procedures has already been suggested for rapid identification certain groups Pseudomonas. However, a comprehensive...

10.3390/microorganisms8081166 article EN cc-by Microorganisms 2020-07-31

The genus Pseudomonas hosts an extensive genetic diversity and is one of the largest genera among Gram-negative bacteria. Type strains are well-known to represent only a small fraction this number available genome sequences increasing rapidly. Consequently, new species regularly reported within in constant evolution. In study, whole se-quencing enabled us define 43 provide update Pseu-domonas evolutionary taxonomic relationships. Phylogenies based on rpoD gene sequences, including 316 313...

10.20944/preprints202107.0335.v1 preprint EN 2021-07-14

Since the discovery of quorum sensing (QS) in 1970s, many studies have demonstrated that Vibrio species coordinate activities such as biofilm formation, virulence, pathogenesis, and bioluminescence, through a large group molecules called N-acyl homoserine lactones (AHLs). However, despite extensive knowledge on involved biological processes controlled by QS few selected strains, less is known about overall diversity AHLs produced broader range environmental strains. To investigate prevalence...

10.3390/s17040906 article EN cc-by Sensors 2017-04-20

Pseudomonas spp. are ubiquitous bacteria able to thrive in a wide range of ecological niches, and lipopeptides often support their lifestyle but also interaction with other micro- macro-organisms. Therefore, the production is widespread among strains.

10.1128/aem.01869-21 article EN cc-by Applied and Environmental Microbiology 2021-11-03

In Pseudomonas lipopeptides, the D-configuration of amino acids is generated by dedicated, dual-function epimerization/condensation (E/C) domains. The increasing attention to stereochemistry in lipopeptide structure elucidation efforts has revealed multiple examples where epimerization does not occur, even though an E/C-type domain present. While origin idle those E/C-domains remains elusive, activity so far shown a binary profile: it either 'on' (active) or 'off' (inactive). Here, we report...

10.3390/ijms241814302 article EN International Journal of Molecular Sciences 2023-09-19

A cellular approach combining Direct Viable Counting and Fluorescent In Situ Hybridization using a one-step multiple-probe technique Solid Phase Cytometry (DVC-FISH-SPC) was developed to monitor total viable vibrios cover the detection of large diversity vibrios. FISH combined three probes in same assay targeted sequences located at different positions on 16S rRNA Vibrio Aliivibrio members. We performed 10-month situ study investigate weekly dynamics relative culturable counts two...

10.1264/jsme2.me17028 article EN Microbes and Environments 2017-01-01

Vibrio fischeri possesses a complex AHL-mediated Quorum-sensing (QS) system including two pathways, LuxI/R (3-oxo-C6-HSL and C6-HSL) AinS/R (C8-HSL), which are important for the regulation of physiological traits. Diverse QS-dependent functional phenotypes have been described in V. fischeri; however, AHL diversity is still underestimated. In present study, we investigated five symbiotic strains with distinct phenotypic properties using UHPLC-HRMS/MS. The results obtained (1) revealed an...

10.1264/jsme2.me18145 article EN Microbes and Environments 2019-01-01

Recent changes in the taxonomy of Pseudomonadaceae family have led to delineation three new genera (Atopomonas, Halopseudomonas and Stutzerimonas). However, genus Pseudomonas remains most densely populated displays a broad genetic diversity. are able produce wide variety secondary metabolites which drives important ecological functions great impact sustaining their lifestyles. While soilborne constantly examined, we currently lack studies aiming explore diversity metabolic potential marine...

10.3389/fmicb.2023.1071039 article EN cc-by Frontiers in Microbiology 2023-04-06

Summary. We tested the benefit of using buffered propionic acid (BPA) as a means preventing farmer's lung disease (FLD). BPA, new formulation acid, hay preservative with no deleterious effect on farm machinery or cattle, reduces development micro‐organisms in hay. Twenty pairs round bales were analysed for concentration measured winter following treatment. Each pair included one untreated bale and treated BPA during haymaking. Our results showed decreases bales: total fungal species, 40% ( P...

10.1046/j.1439-0507.2002.00754.x article EN Mycoses 2002-06-01

The draft genome sequence of wheat rhizosphere isolate Pseudomonas sp. strain SWRI103 is reported. This carries several gene clusters encoding nonribosomal peptide synthetases (NRPSs), including a system for cyclic lipopeptide (CLP) production, and genes carotenoid biosynthesis.

10.1128/mra.00538-20 article EN Microbiology Resource Announcements 2020-07-01

10.1016/0004-6981(68)90001-2 article EN Atmospheric Environment (1967) 1968-07-01

Abstract Cyclic lipopeptides (CLiPs) are secondary metabolites secreted by a range of bacterial phyla. CLiPs from Pseudomonas in particular display diverse structural variations terms the number amino acid residues, macrocycle size, identity and stereochemistry (e.g. D- vs. L-amino acids). Reports detailing discovery novel or already characterized new sources appear regularly literature. Increasingly however, lack detailed characterization threatens to cause considerable confusion,...

10.1101/2022.01.07.475420 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2022-01-09

The draft genome sequence of Pseudomonas aeruginosa LMG 1272, isolated from mushroom, is reported here. This strain triggers formation a precipitate ("white line") when cocultured with tolaasii However, 1272 lacks the capacity to produce cyclic lipopeptide that typically associated white line formation, suggesting involvement different diffusible factor.

10.1128/mra.01363-19 article EN Microbiology Resource Announcements 2020-02-12

Journal Article Method for Analyzing the Effluent from a Microreactor Get access L. Girard, Girard Search other works by this author on: Oxford Academic PubMed Google Scholar A. C. Harkness British Columbia Research Council, Vancouver 8, of Chromatographic Science, Volume 5, Issue 10, October 1967, Pages 516–517, https://doi.org/10.1093/chromsci/5.10.516 Published: 01 1967 history Received: 20 May 1966 Revision received: 10 March

10.1093/chromsci/5.10.516 article EN Journal of Chromatographic Science 1967-10-01
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