A5031136431- Ion Channels and Receptors
- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Child Nutrition and Water Access
- Invertebrate Immune Response Mechanisms
- Public Health and Nutrition
Centre for Inflammation Research
2023
The Queen's Medical Research Institute
2023
University of Edinburgh
2023
Queen Mary University of London
2019-2022
B cells are key pathogenic drivers of chronic inflammation in rheumatoid arthritis (RA). There is limited understanding the relationship between synovial cell subsets and antibody secreting (ASCs). This knowledge crucial for development more targeted B-cell depleting therapies. While CD11c+ double-negative 2 (DN2) have been suggested as an ASC precursor lupus, to date there no proven link two RA. We used both single-cell gene expression BCR sequencing study from patients with established RA,...
Abstract Monocytes are mononuclear phagocytes that can differentiate to a variety of cell fates under the influence their microenvironment and hardwired commitment. We found inhibition TRPM8 in human blood CD14+ monocytes during critical 3-h window at beginning differentiation into macrophages led enhanced survival LPS-driven TNFα production after 24 h. antagonism also promoted derived from intestinal mucosa. Macrophages had been for 6 days blockade impaired phagocytic capacity were...
Abnormal intestinal monocyte to macrophage transition plays a critical role in inflammatory bowel disease (IBD). TRPM8 (Transient Receptor Potential Melastatin 8) is ligand-gated cation channel activated by factors including cold and cooling compounds leading influx. RNA increased both the colonic mucosa of Crohn's patients mice with experimentally induced colitis which activation synthetic agonists ameliorates disease. Mice TRPM8-deficient macrophages develop worse colitis, implying that...
Abstract B cells are key pathogenic drivers of chronic inflammation in rheumatoid arthritis (RA). There is limited understanding the relationship between synovial cell subsets and antibody secreting (ASCs). This knowledge crucial for development targeted therapies. Here, we combine flow cytometry circulating with single-cell RNA paired repertoire sequencing over 27,000 from patients established RA. Twelve clusters were identified including previously recognised subsets, a novel cluster that...