Physiologic activity and community structure of planktonic biofilm microbial communities in an urban river were analyzed using 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) staining, fluorescent situ hybridization (FISH) denaturing gradient gel electrophoresis (DGGE) analysis polymerase chain reaction (PCR)-amplified 16S rDNA fragments.Respiring bacteria estimated by CTC reduction higher biofilms (20%) than stream water samples (12%).FISH revealed that bacterial populations both dominated...

Microbes of low abundance (i.e., rare biosphere) are involved in the ability coral as holobiont, to respond and acclimatize local global environmental disturbances. However, no complete characterization these important microbial assemblages has been conducted. Outlining would greatly benefit our understanding their ecological role coral-microbial association. Here, we assessed bacterial biosphere hermatypic corals Pocillopora cf. damicornis P. verrucosa, well surrounding seawater sediment,...

The key to 650 million years of evolutionary success in jellyfish is adaptability: with alternating benthic and pelagic generations, sexual asexual reproductive modes, multitudes body forms a cosmopolitan distribution, are likely have established plenitude microbial associations. Here we explored bacterial assemblages the scyphozoan Chrysaora plocamia (Lesson 1832). Life stages involved propagation through cyst formation, i.e., mother polyp, its dormant cysts (podocysts), polyps recently...

The northern region of Chile boasts unique geographical features that support the emergence geothermal effluents, salt lagoons, and coastal creeks. These extreme climate conditions create polyextreme habitats for microorganisms, particularly adapted to survive these harsh environments. extremophilic microorganisms hold immense potential as a source hydrolytic enzymes, among other biotechnological applications. In this study, we isolated 15 strains aerobic thermophilic bacteria (45–70 °C)...

The Mexican Central Pacific is located in a zone of oceanographic transition between two biogeographic provinces with particular conditions that affect the associated fauna. objective this study was to evaluate variation hermatypic coral assemblages region and determine their relationship heterogeneity benthonic habitat spatial variables. A total 156 transects were carried out at 41 sites years 2010 2011. sampling effort returned 96.7% richness expected for area, 15 species recorded. results...

This work details a protocol for recovering bacteriophages from intertidal sessile mussels and testing their lytic activity against pathogenic bacteria. Although were highly abundant in coastal filter-feeding organisms, they not detectable the surrounding water column. difference reflects high filtering rate of mussels, which capture concentrate amounts bacteria, generating an ideal environment bacteriophages. We validated providing mean concentration 4E + 04 PFU mL-1 fish-pathogen bacterium...

Bacterial assemblages associated with the hermatypic corals Pocillopora damicornis and P. verrucosa, surrounding seawater sediment at six coral reef sites in north section of Tropical Eastern Pacific were assessed using MiSeq Illumina sequencing V4 region 16S rDNA. The bacterial microbiota both species, stable to seasonal variations. between same substrates not significantly different from each other sampled. Interestingly, composition within site was different, or not, depending on...

In bivalve aquaculture, selecting suitable probiotic treatments can be crucial for improving hatchery-rearing of larvae and juveniles. We assessed the potential five bacterial strains, previously selected in vitro, to improve survival, growth resistance catarina scallop Argopecten ventricosus during early late larval juvenile developmental stages, as well exposure pathogen Vibrio alginolyticus. Hatchery-reared juveniles were treated with eight single or combined strains Bacillus...

The present study describes the isolation of an extremely thermophilic bacterium from El Tatio, a geyser field in high planes Northern Chile. thermophile named Thermus thermophilus strain ET-1 showed 99% identity with T. SGO.5JP 17-16 (GenBank accession No. CP002777) by 16S rDNA gene analysis. Morphologically, cells were non-sporeforming Gram-negative rods that formed colonies yellow pigmentation. This is able to proliferate between 55 and 80 °C pH range 6-10, presenting optimum growth rate...

We isolated microbiota from the digestive tract of Nodipecten subnodosus and Pteria sterna determined in vitro their haemolytic activity, antagonism against Vibrio spp., bacterial hydrophobicity, production extracellular enzymes molecular identification. Five strains were selected: RL5 C3 (Lactobacillus spp.) PB1-1, PB1-5 PB1-6 (Bacillus spp.). The isolates showed antimicrobial activity spp. enzymatic for amylase, protease, lipase cellulose; highly hydrophobic. selected bacteria tested vivo...

Abalone pearl farming is an aquaculture activity with great potential for future growth and diversification of the global sector, which has been dominated decades by pearls marine oysters genus Pinctada. Despite this potential, there a significant lack knowledge regarding methods nucleus (bead) seeding process formation in abalone, compared to well-studied oyster model. To address problem, review first compares main anatomical differences similarities between both groups mollusks,...

The Loa River is the only perennial artery that crosses Atacama Desert in northern Chile. It plays an important role ecological and economic development of most water-stressed region, revealing impact mining industry, which exacerbate regional water shortages for many organisms processes. Despite this, river system has remained understudied. To our knowledge, this study provides first effort to attempt compare microbial communities at spatial scale along River, as well investigate...

Abstract Identifying the core microbiome structure of a metaorganism can be used to monitor impact perturbation against it and changes in its stability (i.e., dysbiosis), resistance, resilience. The core‐microbiome interaction regulates holobiont health homeostasis is an indicator resilience whole community. This study determined exclusive shared taxa two reef‐building coral species ( Pocillopora damicornis P. verrucosa ), as well surrounding seawater sediment, six communities along...

Abstract In shallow sublittoral rocky habitats of the Southeast Pacific, two conspicuous ecological systems exist; kelp beds dominated by large Laminariales algae, and barren grounds crustose coralline algae sea urchins. The aim this study was to examine successional development micro‐periphyton communities in both using a colonization experiment conducted Northern Chile. systems, we installed replicated ceramic plates at 10 m depth samples were taken after 1, 2, 4, 6, 8 14 days exposure....

The biodegradation capacity of planktonic cells and biofilms obtained from a natural river located in an industrial area was compared through the use die-away test. change bacterial community during process also investigated using denaturing gradient gel electrophoresis (DGGE) PCR-amplified 16S rDNA fragments multidimensional scaling (MDS) analysis banding patterns DGGE gel. structure biofilm formed on ceramic slides submerged water were similar to those stones bed, that is, is more suitable...

Physiologic activity and community structure of planktonic biofilm microbial communities in an urban river were analyzed using 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) staining, fluorescent situ hybridization (FISH) denaturing gradient gel electrophoresis (DGGE) analysis polymerase chain reaction (PCR)-amplified 16S rDNA fragments. Respiring bacteria estimated by CTC reduction higher biofilms (20%) than stream water samples (12%). FISH revealed that bacterial populations both dominated...

We designed mathematical first-order difference equations to model the initial stage of bacteriophage infection dynamics in aquatic environments under laboratory conditions. Our only requires knowing bacterial and numbers measure average volume velocity, make predictions about number bacteriophages adsorbed on their host by time. The was validated experimental measurements vitro, showing significant goodness fit when compared curves. This generated as an estimator for effectiveness...

Utilización de una biopelícula