A5051403725- Genomics and Chromatin Dynamics
- Biochemical Analysis and Sensing Techniques
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Advanced Chemical Sensor Technologies
- Catalytic C–H Functionalization Methods
- Olfactory and Sensory Function Studies
- Health, Environment, Cognitive Aging
- Meat and Animal Product Quality
- Food Quality and Safety Studies
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Child Abuse and Trauma
- Metabolomics and Mass Spectrometry Studies
- Single-cell and spatial transcriptomics
- Chromosomal and Genetic Variations
- Migration, Health and Trauma
- Advanced biosensing and bioanalysis techniques
- Genomic variations and chromosomal abnormalities
- Tryptophan and brain disorders
- Renal and related cancers
- Synthesis and Catalytic Reactions
Shanghai Jiao Tong University
2018-2025
Harvard University
2020-2025
Henan University
2024-2025
Nanjing University of Science and Technology
2025
Zhejiang Sci-Tech University
2021-2024
Lawrence Berkeley National Laboratory
2014-2024
Shanghai Children's Hospital
2018-2024
East China University of Science and Technology
2024
Hubei University of Medicine
2024
Harvard University Press
2022-2023
Horizontal gene transfer, in which genetic material is transferred from the genome of one organism to that another, has been investigated microbial species mainly through computational sequence analyses. To address lack experimental data, we studied attempted movement 246,045 genes 79 prokaryotic genomes into Escherichia coli and identified consistently fail transfer. We mechanisms underlying transfer inhibition by placing coding regions different under control inducible promoters. Our data...
Organisms of the third domain life, Archaea, share molecular characteristics both with Bacteria and Eukarya. These organisms attract scientific attention as research models for regulation evolution processes such transcription, translation, RNA processing. We have reconstructed primary transcriptome Sulfolobus solfataricus P2, one most widely studied model archaeal organisms. Analysis 625 million bases sequenced cDNAs yielded a single-base-pair resolution map transcription start sites operon...
Highlights•RNA expression profiling of >12,000 single Arabidopsis root cells by Drop-seq•Recovered major tissue types and cell stages identified specific marker genes•Sucrose alters type proportions but does not profoundly change identities•Identified ∼800 genes dynamically expressed across endodermis developmentSummarySingle-cell transcriptome heterogeneous tissues can provide high-resolution windows into developmental dynamics environmental responses, its application to plants has been...
Ultraconserved elements have been suggested to retain extended perfect sequence identity between the human, mouse, and rat genomes due essential functional properties. To investigate necessities of these in vivo, we removed four noncoding ultraconserved (ranging length from 222 731 base pairs) mouse genome. maximize likelihood observing a phenotype, chose delete that function as enhancers transgenic assay are near genes exhibit marked phenotypes both when completely inactivated their...
Introduction The shape of the face is one most distinctive features among humans, and differences in facial morphology have substantial implications areas such as social interaction, psychology, forensics, clinical genetics. Craniofacial highly heritable, including normal spectrum morphological variation well susceptibility to major craniofacial birth defects. In this study, we explored role transcriptional enhancers development complex. Our study based on rationale that enhancers, which can...
Abstract The etiology of major depressive disorder (MDD), the leading cause worldwide disability, is unknown. neurogenic hypothesis proposes that MDD linked to impairments adult neurogenesis in hippocampal dentate gyrus (DG), while effects antidepressants are mediated by increased neurogenesis. However, alterations and endophenotypes not always causally linked, relationship between altered behavior controversial. To address causality, we used chemogenetics transgenic mice selectively...
Topologically associating domain (TAD) boundaries partition the genome into distinct regulatory territories. Anecdotal evidence suggests that their disruption may interfere with normal gene expression and cause disease phenotypes1-3, but overall extent to which this occurs remains unknown. Here we demonstrate targeted deletions of TAD a range disruptions in vivo function organismal development. We used CRISPR editing mice individually delete eight (11-80 kb size) from genome. All examined...
Aspartic acid (D) and glutamic (E) play vital roles in the umami peptides. To understand their exact mechanism of action, peptides were collected cut into 1/2/3/4 fragments. Connecting D/E to N/C-termini fragments formed consensus effect groups (DEEGs), all containing DEEG summarized according ratio ranking obtained above four situations. The interaction patterns between T1R1/T1R3-VFD compared by statistical analysis molecular docking, most conservative contacts found be HdB_277_ARG...
Determining how an organism responds to its environment by altering gene expression is key understanding ecology. Here, we used RNA-seq comprehensively and quantitatively assess the transcriptional response of bacterial opportunistic cystic fibrosis (CF) pathogen endemic soil dweller, Burkholderia cenocepacia, in conditions mimicking these 2 environments. By sequencing 762 million bases cDNA from closely related B. cenocepacia strains (one isolated a CF patient one soil), identified number...
Abstract Whole-genome sequencing is identifying growing numbers of non-coding variants in human disease studies, but the lack accurate functional annotations prevents their interpretation. We describe genome-wide landscape distant-acting enhancers active developing and adult heart, an organ whose impairment a predominant cause mortality morbidity. Using integrative analysis >35 epigenomic data sets from mouse pre- postnatal hearts we created comprehensive reference >80,000 putative...
Pufferfish is prone to deterioration due abundant nutrients and high moisture content. Drying technology can extend the shelf life enhance flavor quality of aquatic products. The study investigated effect hot air drying (HAD), microwave vacuum (MVD) assisted radio frequency (HARFD) on taste volatile profiles Takifugu obscurus. Different methods had significant influence color, rehydration, 5'-nucleotides, free amino acids components (P < 0.05). results showed that HAD HARFD could promote T....
Roasting is pivotal for enhancing the flavor of Wuyi rock tea (WRT). A study investigated a novel compound that enhances umami taste WRT. Metabolomics Shuixian (SXT) and Rougui (RGT) under light roasting (LR), medium (MR), heavy (HR) revealed significant differences in nonvolatiles compounds. Compared LR reducing sugars amino acids notably decreased MR HR, with l-alanine declining by 69%. Taste-guided fractionation identified fraction II–B as having high sweet intensities. surprising...
Abstract The genetic basis of human facial variation and craniofacial birth defects remains poorly understood. Distant-acting transcriptional enhancers control the fine-tuned spatiotemporal expression genes during critical stages development. However, a lack accurate maps genomic locations cell type-resolved activities prevents their systematic exploration in genetics studies. Here, we combine histone modification, chromatin accessibility, gene profiling development with single-cell analyses...