A5034248184- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Metalloenzymes and iron-sulfur proteins
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- Biochemical Acid Research Studies
- Microbial metabolism and enzyme function
Institute of Biological Chemistry, Academia Sinica
2022-2024
Abstract Biological CO 2 fixation is so far the most effective means for reduction at scale and accounts of fixed on Earth. Through this process, carbon in cellular components biomass during organismal growth. To uncouple from growth regulation, cell-free systems represent an alternative approach since rate can be independently manipulated. Here we designed oxygen-insensitive, self-replenishing system with opto-sensing. The comprises a synthetic reductive glyoxylate pyruvate synthesis (rGPS)...
As methanol can be derived from either CO2 or methane, economy play an important role in combating climate change. In this scenario, rapid utilization of by industrial microorganism is the first and crucial step for efficient C1 feedstock chemical. Here, we report development a methylotrophic E. coli strain with doubling time 3.5 hours under optimal conditions, comparable faster than native model methylotrophs Methylorubrum extorquens AM1 (Td~4hr) Bacillus methanolicus at 37°C (Td~5hr). To...
Abstract As methanol can be derived from either CO 2 or methane, economy may play a role in combating climate change. In this scenario, rapid utilization of by an industrial microorganism is the first and crucial step for efficient C1 feedstock chemical. Here, we report development methylotrophic E. coli strain (SM6) with doubling time 3.5 hours, outpacing that common native methylotrophs. We accomplish using evolution engineering dynamic copy number variation (CNV). developed bacterial...