A5067003073- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Uterine Myomas and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Endometrial and Cervical Cancer Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Macrophage Migration Inhibitory Factor
- Protease and Inhibitor Mechanisms
- Estrogen and related hormone effects
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cardiovascular Issues in Pregnancy
- Gynecological conditions and treatments
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- HIV Research and Treatment
- Perfectionism, Procrastination, Anxiety Studies
- Signaling Pathways in Disease
- Pregnancy-related medical research
- Cancer-related molecular mechanisms research
- Nutrition, Genetics, and Disease
- Nuclear Receptors and Signaling
- Extracellular vesicles in disease
- Omental and Epiploic Conditions
The University of Kansas Cancer Center
2024
University of Kansas Medical Center
2015-2024
Reproductive Science Center
2023
The University of Kansas Health System
2023
University of Kansas
1998-2018
Institute for Reproductive Health
2015-2017
University of Kentucky
1994-1999
Central Baptist Hospital
1994
The Ohio State University
1990
The ribonuclease III endonuclease, Dicer1 (also known as Dicer), is essential for the synthesis of 19-25 nucleotide noncoding RNAs micro-RNAs (miRNAs). These miRNAs associate with RNA-induced silencing complex to regulate gene expression posttranscriptionally by base pairing 3'untranslated regions complementary mRNA targets. Although it established that are expressed in reproductive tract, their functional role and effect on disease remain unknown. studies herein establish first time...
Uterine fibroids (leiomyomas) are the most common tumors of female reproductive tract, occurring in up to 77% reproductive-aged women, yet molecular pathogenesis remains poorly understood. A role for atypically activated mammalian target rapamycin (mTOR) pathway uterine has been suggested several studies. We identified that G protein-coupled receptor 10 [GPR10, a putative signaling protein upstream phosphoinositide 3-kinase–protein kinase B/AKT–mammalian (PI3K/AKT–mTOR) pathway] is...
In many species, including the rabbit, spermatozoa attach to apical plasma membrane of oviductal epithelial cells. Attachment cells has a beneficial effect on maintenance sperm viability during storage in oviduct, but mechanism by which this occurs is unknown. The present study was conducted determine role direct contact between and maintaining viability. To accomplish this, an fraction isolated from homogenized rabbit oviducts kidney (control) differential precipitation. When viewed...
SUMMARY ANSWERmiR451 expression was elevated in endometriotic lesion tissue. MiR451 modulated the of macrophage migration inhibitory factor and limited cell survival.
In this review, we focus on recent advancements in our understanding of the roles inflammatory mediators endometriosis pathophysiology and potential for improved therapies based upon targeting these pathways. We review association between inflammation initial promise anti-tumor necrosis factor experimental evidence, how why studies have not translated to clinic. then discuss emerging data role inter-relationship among macrophage migration inhibitory factor, prostaglandin E 2, estrogen...
Interleukin-1 (IL-1) is a polypeptide that has both local and systemic effects on numerous tissues, including endocrine cells. To evaluate the effect of IL-1 luteal function, bovine cells were cultured for 5 days with increasing concentrations (0.1, 0.5, 1.0, 2.5, 5.0, 10.0 ng/ml) recombinant interleukin-1 β (rbIL-1 β). increased production 6-keto-prostaglandin-F1 α (6-keto-PGF1 α), prostaglandin E2 (PGE2), F2 (PGF2 α) in dose-dependent manner, but had no progesterone (P4) production....
Abstract Background Mutations in the receptor tyrosine kinase gene fibroblast growth factor 2 ( FGFR2 ) occur at a high frequency endometrial cancer (EC) and have been linked to advanced recurrent disease. However, little is known about how these mutations drive carcinogenesis. Methods Differential transcriptomic analysis two‐step quantitative real‐time PCR (qRT‐PCR) assays were applied identify genes differentially expressed two cohorts of EC patients carrying as well cells harbouring ....
Tissue inhibitor of metalloproteinase (TIMP)-1 is a multifunctional peptide that has been implicated in the ovulatory process. To assess function TIMP-1 during periovulatory period vivo, mice incapable expressing gene product were utilized. Twenty-three-day-old TIMP-1-deficient (n = 59) and wild-type 61) female injected with 5 IU eCG, followed 48 h later by an ovulation-inducing dose hCG (5 IU). Animals killed at time injection (0-h hCG), 12 (12-h or 24 post-hCG (24-h hCG) administration....
Tissue inhibitor of metalloproteinase-1 (TIMP-1) is a multifunctional protein expressed in the uterus essentially all species, yet function this uncertain. To assess role TIMP-1 uterine events that occur during murine estrous cycle, mature female wild-type and null mice were monitored for reproductive cyclicity. Mice sacrificed each stage peripheral blood was collected assayed serum estradiol progesterone content by RIA. Uterine morphology TIMP-1, TIMP-2, TIMP-3, TIMP-4 mRNA expression also...
PROBLEM: The immune system has been implicated in the pathophysiology of endometriosis. To determine if modulation influences endometriotic implant growth and protein production, following experiment was conducted. METHOD: Female rats with surgically induced endometriosis were treated either immunosuppressive agent pentoxifylline (Pent; 5 mg/kg BW; N = 16) or a vehicle (N for 7 consecutive days, then killed. Twenty‐four hours before death, 8 animals from each group injected intraperitoneally...
The present study examined the role of tissue inhibitors metalloproteinases ITIMPs) in remodeling that occurs during luteal development and regression throughout pseudopregnancy rat. Pseudopregnancy was induced immature female rats by eCG/ hCG priming. Animals (n = 4 per time point) were killed on Days 1, 2, 4, 8, 12, 14, 16 (post administration), ovaries removed analyzed for metalloproteinase inhibitor activity or TIMP-1, TIMP-2, TIMP-3 mRNA expression. Inhibitory highest Day-1 samples...
Endometriosis is defined as the growth of endometrial glandular and stromal components in ectopic locations affects many 10% all women reproductive age. Despite its high prevalence, pathogenesis endometriosis remains poorly understood. MicroRNAs, small non-coding RNAs that post-transcriptionally regulate gene expression, are mis-expressed but a functional role disease uncertain. To examine microRNA-451 (miR-451) initial development endometriosis, we utilized novel mouse model which eutopic...
In the present study, we examined roles of matrix metalloproteinases collagenase, 72-kDa and 92-kDa gelatinase, proteoglycanase in tissue remodeling that occurs during luteal development regression, using a pseudopregnant rat model. Pseudopregnancy was induced immature female rats by eCG/hCG priming, animals (n = 3 to 4 per time point) were killed on Days 1, 2, 4, 8, 12, 14, or 16 pseudopregnancy (Day 0 hCG administration). Ovaries then removed analyzed for either metalloproteinase mRNA...