A5074537385- Lipid Membrane Structure and Behavior
- Surfactants and Colloidal Systems
- Supramolecular Self-Assembly in Materials
- Polymer Surface Interaction Studies
- Advanced Polymer Synthesis and Characterization
- RNA and protein synthesis mechanisms
- Cellular Mechanics and Interactions
- Microbial metabolism and enzyme function
- Enzyme Structure and Function
- RNA modifications and cancer
- interferon and immune responses
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- Cell death mechanisms and regulation
Utrecht University
2023-2024
University of Tübingen
2023
Institute of Molecular Biology and Biophysics
2015-2017
University of Kaiserslautern
2015-2016
The dynamic ribosome-translocon complex, which resides at the endoplasmic reticulum (ER) membrane, produces a major fraction of human proteome1,2. It governs synthesis, translocation, membrane insertion, N-glycosylation, folding and disulfide-bond formation nascent proteins. Although individual components this machinery have been studied high resolution in isolation3-7, insights into their interplay native remain limited. Here we use cryo-electron tomography, extensive classification...
Abstract BAX and BAK are proapoptotic members of the BCL2 family that directly mediate mitochondrial outer membrane permeabilition (MOMP), a central step in apoptosis execution. However, molecular architecture apoptotic pore remains key open question especially little is known about contribution lipids to MOMP. By performing comparative lipidomics analysis proximal environment isolated lipid nanodiscs, we find significant enrichment unsaturated species nearby conditions. We then demonstrate...
Copolymers of styrene and maleic acid (SMA) have gained great attention as alternatives to conventional detergents, they offer decisive advantages for studying membrane proteins lipids in vitro. These polymers self-insert into artificial biological membranes and, at sufficiently high concentrations, solubilise them disc-shaped nanostructures containing a lipid bilayer core surrounded by polymer belt. We used 31P nuclear magnetic resonance spectroscopy dynamic light scattering systematically...
Some styrene/maleic acid (SMA) copolymers solubilise membrane lipids and proteins to form polymer-bounded nanodiscs termed SMA/lipid particles (SMALPs). Although SMALPs preserve a lipid-bilayer core, they appear be more dynamic than other mimics. We used time-resolved Förster resonance energy transfer small-angle neutron scattering determine the kinetics mechanisms of phospholipid among SMALPs. In contrast with vesicles or protein-bounded nanodiscs, exchange not only by monomer diffusion but...
The lipid interactions of unconventional surfactants are conceptualised in terms a three-stage model, allowing for new thermodynamic classification.
Membrane proteins are an essential part of signaling and transport processes targeted by multiple drugs. To isolate investigate them in their native state, polymer-bounded nanodiscs have become valuable tools. In this study, we the lipid model system dimyristoyl-phosphocholine (DMPC) with nanodisc-forming copolymers styrene maleic acid (SMA) diisobutylene (DIBMA). Using small-angle X-ray scattering (SAXS) dynamic light (DLS), studied influence polymer concentration temperature on nanodisc...