A5082762622- Prenatal Screening and Diagnostics
- Reproductive Biology and Fertility
- Chromosomal and Genetic Variations
- Sperm and Testicular Function
- Assisted Reproductive Technology and Twin Pregnancy
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomic variations and chromosomal abnormalities
- Genomics and Phylogenetic Studies
- Renal and related cancers
- Innovations in Medical Education
- DNA Repair Mechanisms
- Clinical Reasoning and Diagnostic Skills
- RNA and protein synthesis mechanisms
- Problem and Project Based Learning
- Reproductive Health and Technologies
- Pluripotent Stem Cells Research
- Telomeres, Telomerase, and Senescence
- bioluminescence and chemiluminescence research
- COVID-19 Impact on Reproduction
- Ovarian function and disorders
- Genetics, Bioinformatics, and Biomedical Research
- Carcinogens and Genotoxicity Assessment
- Advanced biosensing and bioanalysis techniques
Roseman University of Health Sciences
2021-2025
Florida International University
2012-2021
Molecular Research Institute
2018
Reproductive Biology Associates
2013
University of Calgary
2007-2010
University of Kent
2004-2009
London Bridge Hospital
2009
University of Cambridge
2009
Brunel University of London
2003-2004
Birds are characterised by feathers, flight, a small genome and very distinctive karyotype. Despite the large numbers of chromosomes, diploid count 2n ≈ 80 has remained remarkably constant with 63% birds where = 74–86, 24% 66–74 extremes 40 142. Of these, most studied is chicken (2n 78), molecular cytogenetic probes generated from this species have been used to further understand evolution avian genome. The ancestral karyotype is, it appears, similar that chicken, chromosomes 1, 2, 3, 4q, 5,...
Abstract Chicken genome mapping is important for a range of scientific disciplines. The ability to distinguish chromosomes the chicken and other birds thus priority. Here we describe molecular cytogenetic characterization each chromosome using painting individual clones by FISH. Where possible, have assigned known linkage groups. We propose, on basis size, that NOR approximately size 22; however, suggest its original assignment 16 should be retained. also definitive classification system...
Abstract Background Comparative genomics is a powerful means of establishing inter-specific relationships between gene function/location and allows insight into genomic rearrangements, conservation evolutionary phylogeny. The availability the complete sequence chicken genome has initiated development detailed information in other birds including turkey, an agriculturally important species where mapping hitherto focused on linkage with limited physical information. No molecular study yet...
Multicolour fluorescent in situ hybridization was utilized to detect sperm aneuploidy for chromosomes 13, 21, X and Y testicular cancer Hodgkin's lymphoma chemotherapy patients. Aneuploidy assessed before, 6, 12 and/or 18–24 months after, the initiation of chemotherapy, compared with age matched controls. 635 396 were scored blindly 5000 sperm/patient/chromosome/ time point, where available. (First two phrases have been reversed). Comparing patients each other controls, cancer-specific...
The availability of the complete chicken (Gallus gallus) genome sequence as well a large number probes for fluorescent in-situ hybridization (FISH) and microarray resources facilitate comparative genomic studies between other bird species. In previous study, we provided comprehensive cytogenetic map turkey (Meleagris gallopavo) first analysis copy variants (CNVs) in birds. Here, extend this approach to Pekin duck (Anas platyrhynchos), an obvious target due its agricultural importance...
BACKGROUNDWe present the first powered prospective study to assess whether sperm aneuploidy can predict outcome of ICSI.
Single cell gel electrophoresis, also known as the comet assay, has become a widespread DNA damage assessment tool due to its sensitivity, adaptability, low cost, ease of use, and reliability. Despite these benefits, this assay shortcomings, such long running time, manipulation multiple slides, individually, through numerous process steps, challenge working in darkened environment, reportedly considerable inter- intra-laboratory variation. All researchers typically perform based upon common...
Abstract The organization of chromosomes in sperm nuclei has been proposed to possess a unique “hairpin-loop” arrangement, which is hypothesized aid the ordered exodus paternal genome following fertilization. This study simultaneously assessed 3D and 2D radial longitudinal telomeres, centromeres, investigated whether formed same centromere clusters cells. Reproducible non-random was observed for all loci using both approaches multiple subjects. We report novel findings, with telomeres...
In this study Chinese herbs commonly used in the treatment of male infertility were investigated for relevant biochemical activity. Male factor predominantly arises via barriers to, or defects in, spermatogenesis. The process spermatogenesis is under strict endocrine control; addition oxidative stress has been implicated with significant levels reactive oxygen species detected 25% infertile males. A total 37 individual and seven herb decoctions therefore tested activity using a recombinant...