A5087121657- Chromosomal and Genetic Variations
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- RNA and protein synthesis mechanisms
- Genetic and phenotypic traits in livestock
- Livestock and Poultry Management
- Agriculture and Rural Development Research
- Animal Nutrition and Physiology
- Plant Reproductive Biology
- Rabbits: Nutrition, Reproduction, Health
- Genetic diversity and population structure
- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Peroxisome Proliferator-Activated Receptors
- Glycosylation and Glycoproteins Research
- Education, sociology, and vocational training
- Health, Medicine and Society
- Agroforestry and silvopastoral systems
- Plant Virus Research Studies
- Ruminant Nutrition and Digestive Physiology
- Silkworms and Sericulture Research
- Agriculture Sustainability and Environmental Impact
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2008-2024
Génétique Physiologie et Systèmes d'Elevage
2014-2024
Laboratoire de Génétique Cellulaire
2004-2024
Laboratoire de Génie Chimique
2024
Université de Toulouse
2017-2023
École Nationale Vétérinaire de Toulouse
2019-2023
Hospices Civils de Lyon
2015
Institut National de la Recherche Agronomique
1999-2013
RELX Group (Netherlands)
1998
Centre National de la Recherche Scientifique
1998
Abstract The importance of the Gallus gallus (chicken) as a model organism and agricultural animal merits continuation sequence assembly improvement efforts. We present new version chicken genome (Gallus_gallus-5.0; GCA_000002315.3), built from combined long single molecule sequencing technology, finished BACs, improved physical maps. In overall assembled bases, we see gain 183 Mb, including 16.4 Mb in placed chromosomes with corresponding percentage intact repeat elements characterized. Of...
Rose-comb, a classical monogenic trait of chickens, is characterized by drastically altered comb morphology compared to the single-combed wild-type. Here we show that Rose-comb caused 7.4 Mb inversion on chromosome 7 and second allele arose unequal crossing over between wild-type chromosome. The phenotype relocalization MNR2 homeodomain protein gene leading transient ectopic expression during development. We also provide molecular explanation for first example epistatic interaction reported...
Abstract Chicken genome mapping is important for a range of scientific disciplines. The ability to distinguish chromosomes the chicken and other birds thus priority. Here we describe molecular cytogenetic characterization each chromosome using painting individual clones by FISH. Where possible, have assigned known linkage groups. We propose, on basis size, that NOR approximately size 22; however, suggest its original assignment 16 should be retained. also definitive classification system...
Abstract Background Comparative genomics is a powerful means of establishing inter-specific relationships between gene function/location and allows insight into genomic rearrangements, conservation evolutionary phylogeny. The availability the complete sequence chicken genome has initiated development detailed information in other birds including turkey, an agriculturally important species where mapping hitherto focused on linkage with limited physical information. No molecular study yet...
By comparing the quail genome with that of chicken, chromosome rearrangements have occurred in these two galliform species over 35 million years evolution can be detected. From a more practical point view, definition conserved syntenies helps to predict position genes quail, based on information taken from chicken sequence, thus enhancing utility this biological studies through better knowledge its structure. A microsatellite and an Amplified Fragment Length Polymorphism (AFLP) genetic map...
As a first step towards the development of radiation hybrid maps, we have produced panel in chicken by fusing female embryonic diploid fibroblasts irradiated at 6 000 rads with HPRT-deficient hamster Wg3hCl2 cells. Due to low retention frequency fragments, high number clones was from which best ones were selected. Thus, 452 fusion tested for frequencies 46 markers. Based on these results, 103 mean marker 23.8% selected large scale culture produce DNA sufficient quantities genotyping numerous...
Abstract Background The chicken karyotype is composed of 39 chromosome pairs, which 9 still remain totally absent from the current genome sequence assembly, despite international efforts towards complete coverage. Some others are only very partially sequenced, amongst microchromosome 16 (GGA16), particularly under-represented, with 433 kb assembled for a full estimated size to 11 Mb. Besides obvious need coverage genetic markers QTL (Quantitative Trait Loci) mapping and major genes...
The chicken MHC is organized in two genetically independent gene complexes <i>B</i><sup>@</sup>and <i>RFP-Y</i>®<i>. </i>Previous studies have shown the localization of <i>B</i><sup>@</sup>complex on a small microchromosome. By using two-color fluorescent situ hybridization, we demonstrate <i>RFP-Y</i><sup>@</sup> complex to same chromosome. A recombination hot spot between loci might account for...
Le replacement consiste en l’adoption ou le placement dans des structures spécialisées, d’animaux utilisés à fins scientifiques une fois protocole expérimental achevé. Ce principe de réhabilitation a été intégré tant que 4e R la directive européenne sur l’expérimentation animale ainsi Code rural et pêche maritime dont l’article 214-112 définit les conditions remplir. En privilégiant maintien vie animaux, est démarche éthique alternative l’euthanasie. Pour mener bien processus replacement, il...
Interview de Laurence FORTUN-LAMOTHE, Davi SAVIETTO, Valérie FILLON
Owing to the low cost of high throughput Next Generation Sequencing (NGS) technology, more and species have been will be sequenced. However, de novo assemblies large eukaryotic genomes thus produced are composed a number contigs scaffolds medium small size, having no chromosomal assignment. Radiation hybrid (RH) mapping is powerful tool for building whole genome maps has used several animal species, help assign sequence chromosomes determining their order.We report here duck RH panel...
In quail, two feather colour phenotypes i.e. fawn-2/beige and yellow are associated with the ASIP locus. The aim of our study was to characterize structural modifications within this locus that explain mutation (large deletion) (assumed be caused by a different modification).For phenotype, we identified complex involves 141,162-bp long deletion. For 71-kb tandem duplication comprises one unchanged copy present in ITCH-ASIP fusion gene, which leads transcript coding for normal protein....
The aim of this article is not to present an exhaustive review molecular cytogenetics applications in domestic animal species, but more illustrate the considerable contribution these approaches diagnostics and research economically important species. A short presentation main humans points out domains which it has become essential tool underlines specificities attached species comparison farm animals. This devoted outlining current resources available some examples fluorescence situ...
Summary Karyotypes of chicken ( Gallus gallus domesticus ; 2 n = 78) and mallard duck Anas platyrhynchos 80) share the typical organization avian karyotypes including a few macrochromosome pairs, numerous indistinguishable microchromosomes, Z W sex chromosomes. Previous banding studies revealed great similarities between chickens ducks, but it was not possible to use comparative for microchromosomes. In order establish precise chromosome correspondences these two species, particularly we...
Retinal progenitor cells undergo apical mitoses during the process of interkinetic nuclear migration and newly generated post-mitotic neurons migrate to their prospective retinal layer. Whereas this is valid for most types neurons, chicken horizontal are by delayed non-apical from dedicated progenitors. The regulation such final cell cycle not well understood we have studied how Lim1 expressing (HPCs) exit cycle. We used markers S- G2/M-phase in combination with regulators Rb1, cyclin B1,...
Providing rabbits with a grassy outdoor area allows them to express broad variety of specific behaviours such as grazing where grazeable herbage persists. However, that graze are also exposed external stressors. Controlled access time may help preserve the grassland resource, while hiding place offer secure space. We focused on rabbit growth, health and behaviour according presence hideout 30-m2 pasture area. divided 144 into four groups (group 8 hours per day (H8) pastures provided an (Y)...
Providing rabbits with outdoor access allows them to express a large repertoire of behaviours and addresses societal expectations surrounding animal welfare in livestock systems. The aim this work was study rabbit growth, health, behaviour according genetic type pasture area size. We distributed 192 weaned into two groups different sizes, i.e., (LP): 60 m2 (n = 4 × 24) small (SP): 30 24). Each group contained half Californian 1001 New Zealand 1777 (1001) PS119 (PS119). Rabbits were reared...
The ChickRH6 radiation hybrid panel has been used to construct consensus chromosome (RH) maps of the chicken genome. Markers genotyped were either from throughout genome or targeted specific chromosomes and a large proportion (one third) data was result collaborative efforts. Altogether, 2,531 markers genotyped, allowing construction RH reference for 20 linkage groups four other chromosomes. Amongst markers, 581 belong framework maps, while 1,721 are on comprehensive maps. Around 800 still...
Intensive animal production is facing a crisis of legitimacy linked to its contribution pollution, biohazard risks, and suffering. With almost 97% the coming from intensive systems, rabbit farming questioned. Similarly, plant sector under scrutiny high input dependency. Among alternatives, organic agroforestry systems (associating trees animals) may contribute more sustainable agriculture. However, number elements should be evaluated when designing especially innovative with no previous...
Abstract Background The publication of the first draft chicken sequence assembly became available in 2004 and was updated 2006. However, this does not constitute a definitive complete genome, since microchromosomes are notably under-represented. In an effort to develop maps for absent from genome assembly, we developed radiation hybrid (RH) genetic with markers isolated currently assigned "chromosome Unknown" (chrUn). chrUn is composed contigs named chromosomes. To identify map belonging...
As an approach to integrate the chicken genetic and cytogenetic maps, bacterial artificial chromosome (BAC) P1‐derived (PAC) clones were localized by fluorescence in situ hybridization (FISH) on chromosomes mapping East Lansing Compton reference families. Some of used this study previously selected for presence potentially polymorphic (CA)n repeats a microsatellite marker was developed when possible mapping. For other clones, single strand conformational polymorphism (SSCP) purpose. Between...