A5003053481- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- 14-3-3 protein interactions
- Plant and Biological Electrophysiology Studies
- Plant Molecular Biology Research
- Nitrogen and Sulfur Effects on Brassica
- Mitochondrial Function and Pathology
- Fungal and yeast genetics research
- Magnetic and Electromagnetic Effects
- Ubiquitin and proteasome pathways
- Genomics and Phylogenetic Studies
- Hybrid Renewable Energy Systems
- Microbial Metabolic Engineering and Bioproduction
- Plant Reproductive Biology
- Bioinformatics and Genomic Networks
- Genetic Neurodegenerative Diseases
- Various Chemistry Research Topics
- Transgenic Plants and Applications
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- RNA and protein synthesis mechanisms
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
- Plant biochemistry and biosynthesis
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022
Centre National de la Recherche Scientifique
2005-2022
Institut des Sciences des Plantes de Montpellier
2008-2022
Institut Agro Montpellier
2008-2022
Université de Montpellier
2008-2022
UCLouvain
2000-2014
Centre National pour la Recherche Scientifique et Technique (CNRST)
2011
Yeasty HIPHOP In order to identify how chemical compounds target genes and affect the physiology of cell, tests perturbations that occur when treated with a range pharmacological chemicals are required. By examining haploinsufficiency profiling (HIP) homozygous (HOP) chemogenomic platforms, Lee et al. (p. 208 ) analyzed response yeast thousands different small molecules, genetic, proteomic, bioinformatic analyses. Over 300 were identified targeted 121 within 45 cellular signature networks....
The plasma membrane proton pump ATPase (H+-ATPase) plays a major role in the activation of ion and nutrient transport has been suggested to be involved several physiological processes, such as cell expansion salt tolerance. Its activity is regulated by C-terminal autoinhibitory domain that can displaced phosphorylation binding regulatory 14-3-3 proteins, resulting an activated enzyme. To better understand consequence this activation, we have analyzed transgenic tobacco (Nicotiana tabacum)...
Summary Amongst the nine voltage‐gated K + channel (Kv) subunits expressed in Arabidopsis, AtKC1 does not seem to form functional Kv channels on its own, and is therefore said be silent. It has been proposed a regulatory subunit, significantly influence properties of heteromeric which it participates, along with other subunits. The mechanisms underlying these remain unknown. Here, transient (co‐)expression , AKT1 and/or KAT1 genes was obtained tobacco mesophyll protoplasts, lack endogenous...
A functional Shaker potassium channel requires assembly of four α-subunits encoded by a single gene or various genes from the family. In Arabidopsis thaliana, AtKC1, α-subunit that is silent when expressed alone, has been shown to regulate activity AKT1 forming heteromeric AtKC1-AKT1 channels. Here, we investigated whether AtKC1 general regulator activity. Co-expression in Xenopus oocytes dominant negative (pore-mutated) subunit with inward subunits KAT1, KAT2 AKT2, outward SKOR GORK,...
Friedreich's ataxia is caused by a deficit in frataxin, small mitochondrial protein of unknown function that has been conserved during evolution. Previous studies have pointed out role for frataxin iron-sulfur (Fe-S) metabolism. Here, we analyzed the incorporation Fe-S clusters into yeast ferredoxin imported isolated energized mitochondria from cells grown presence glycerol, an obligatory respiratory carbon source. Similar amounts apo-ferredoxin precursor were and processed wild-type...
The proton pump ATPase (H<sup>+</sup>-ATPase) of the plant plasma membrane is regulated by an autoinhibitory C-terminal domain, which can be displaced phosphorylation penultimate Thr residue and subsequent binding 14-3-3 proteins. We performed a mass spectrometric analysis PMA2 (plasma H<sup>+</sup>-ATPase isoform 2) isolated from <i>Nicotiana tabacum</i> suspension cells identified two new phosphorylated residues in enzyme protein site: Thr<sup>931</sup> Ser<sup>938</sup>. When was...
Abstract Stomata optimize land plants’ photosynthetic requirements and limit water vapor loss. So far, all of the molecular electrical components identified as regulating stomatal aperture are produced, operate, directly within guard cells. However, a completely autonomous function cells is inconsistent with anatomical biophysical observations hinting at mechanical contributions epidermal origins. Here, potassium (K+) assays, membrane potential measurements, microindentation, plasmolysis...
The plant plasma membrane H+-ATPase is regulated by an auto-inhibitory C-terminal domain that can be displaced phosphorylation of the penultimate residue, a Thr, and subsequent binding 14-3-3 proteins. By mass spectrometric analysis isoform 2 (PMA2) isolated from Nicotiana tabacum plants suspension cells, we identified new site, Thr-889, in region upstream protein site. This residue was mutated into aspartate or alanine, H+-ATPases expressed yeast Saccharomyces cerevisiae. Unlike wild-type...
The recessive nuclear vdl (for variegated and distorted leaf) mutant of tobacco was obtained by T-DNA insertion characterized leaves abnormal roots flowers. Affected leaf tissues were white distorted, lacked palisadic cells, contained undifferentiated plastids. variegation due to phenotypic, rather than genetic, instability. Genomic cDNA clones for both the wild-type VDL alleles. Three transcripts, resulting from alternate intron splicing or polyadenylation, found wild type. transcripts...
Summary A deletion and mutagenesis study was performed on the mitochondrial presequence of β‐subunit F 1 ‐ATP synthase from Nicotiana plumbaginifolia linked to green fluorescent protein (GFP). The various constructs were tested in vivo by transient expression tobacco protoplasts. GFP distribution transformed cells analysed situ confocal microscopy, vitro subcellular fractions Western blotting. Despite its being highly conserved different species, C‐terminal region (residues 48–54) did not...
The structural determinants involved in the targeting of multitransmembrane-span proteins to plasma membrane (PM) remain poorly understood. H+ -ATPase (PMA) from Nicotiana plumbaginifolia, a well-characterized 10 transmembrane-span enzyme, was used as model identify elements essential for PM. When PMA2 and PMA4, representatives two main PMA subfamilies, were fused green fluorescent protein (GFP), chimeras shown be still functional correctly rapidly targeted PM transgenic tobacco. By...
The plasma membrane H(+)-ATPases PMA2 and PMA4 are the most widely expressed in Nicotiana plumbaginifolia, belong to two different subfamilies. Both activated by phosphorylation of a Thr at penultimate position subsequent binding 14-3-3 proteins. Their expression Saccharomyces cerevisiae revealed functional regulatory differences. To determine whether properties between exist plants, we generated monoclonal antibodies able detect either or total protein extract. We also raised tabacum...
Potassium translocation in plants is accomplished by a large variety of transport systems. Most the available molecular information on these proteins concerns voltage-gated potassium channels (Kv channels). The Arabidopsis genome comprises nine genes encoding alpha-subunits Kv channels. Based knowledge their animal homologues, and biochemical investigations, it broadly admitted that four such polypeptides must assemble to yield functional channel. intrinsic properties channel have been...
Abstract Background In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made characterise such putative at the functional level most often relied electrophysiological in classical expression systems, as Xenopus oocytes or mammalian cells. cases, these systems failed so far provide data and one can speculate that using system instead an might more efficient way towards characterisation...
The Nicotiana tabacum Bright-Yellow-2 (BY2) cell line is one of most commonly used plant suspension lines and offers interesting properties, such as fast growth, amenability to genetic transformation, synchronization division. To build a proteome reference map BY2 proteins, we isolated the soluble proteins from N. cells at end exponential growth phase analyzed them by 2-DE MALDI TOF-TOF. Of 1422 spots isolated, 795 were identified with significant score, corresponding 532 distinct proteins.
We sought to determine the structural features involved in processing of mitochondrial F 1 ‐ATPase β‐subunit (F β) presequence (54 residues) from Nicotiana plumbaginifolia . The cleavage efficiency β mutants linked green fluorescent protein (GFP) was evaluated vivo tobacco by situ microscopy and Western blotting. residue at position −1 (Tyr) required be an aromatic +2 (Thr) found important for processing, while, unexpectedly, changing distal (Arg‐15) proximal (Arg‐5) arginine residues did...