A5021980299- Gene expression and cancer classification
- Fungal and yeast genetics research
- Molecular Biology Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Bioinformatics and Genomic Networks
- Evolution and Genetic Dynamics
- Genomics and Chromatin Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Genetics, Bioinformatics, and Biomedical Research
- Various Chemistry Research Topics
- RNA and protein synthesis mechanisms
- Law, AI, and Intellectual Property
- Lichen and fungal ecology
- Helminth infection and control
- Microbial Natural Products and Biosynthesis
- Genetics, Aging, and Longevity in Model Organisms
- Mycorrhizal Fungi and Plant Interactions
- Antimicrobial Peptides and Activities
- Parasite Biology and Host Interactions
- Spaceflight effects on biology
- Intellectual Property and Patents
- Fungal Plant Pathogen Control
- Biochemical and Structural Characterization
- Parasites and Host Interactions
- Light effects on plants
University of California, Los Angeles
2025
University of British Columbia
2014-2017
Yeasty HIPHOP In order to identify how chemical compounds target genes and affect the physiology of cell, tests perturbations that occur when treated with a range pharmacological chemicals are required. By examining haploinsufficiency profiling (HIP) homozygous (HOP) chemogenomic platforms, Lee et al. (p. 208 ) analyzed response yeast thousands different small molecules, genetic, proteomic, bioinformatic analyses. Over 300 were identified targeted 121 within 45 cellular signature networks....
Abstract While the pace of discovery human genetic variants in tumors, patients, and diverse populations has rapidly accelerated, deciphering their functional consequence become rate-limiting. Using cross-species complementation, model organisms like budding yeast, Saccharomyces cerevisiae, can be utilized to fill this gap serve as a platform for testing variants. To end, we performed two parallel screens, one-to-one complementation screen essential yeast genes implicated chromosome...
Background The lack of new anthelmintic agents is growing concern because it affects human health and our food supply, as both livestock plants are affected. Two principal factors contribute to this problem. First, nematode resistance drugs increasing worldwide second, many effective nematicides pose environmental hazards. In paper we address problem by deploying a high throughput screening platform for drug discovery using the Caenorhabditis elegans surrogate infectious nematodes. This...
Systematic analyses of essential gene function using mutant collections in Saccharomyces cerevisiae have been conducted heterozygous diploids, promoter shut-off alleles, through alleles with destabilized mRNA, protein, or bearing mutations that lead to a temperature-sensitive (ts) phenotype. We previously described method for construction barcoded ts systematic fashion. Here we report the completion this collection covering 600 yeast genes. This resource covers larger repertoire than...
Identifying additional immune checkpoints hindering antitumor T cell responses is key to the development of next-generation cancer immunotherapies. Here, we report induction serotonin transporter (SERT), a regulator levels and physiological functions in brain peripheral tissues, tumor-infiltrating CD8 cells. Inhibition SERT using selective reuptake inhibitors (SSRIs), most widely prescribed antidepressants, significantly suppressed tumor growth enhanced immunity various mouse syngeneic human...
The Yeast Knockout (YKO) collection has provided a wealth of functional annotations from genome-wide screens. An unintended consequence is that 76% gene derive one genotype. nutritional auxotrophies in the YKO, particular, have phenotypic consequences. To address this issue, ‘prototrophic’ versions YKO been constructed, either by introducing plasmid carrying wild-type copies auxotrophic markers (Plasmid-Borne, PB prot ) or backcrossing (Backcrossed, BC to strain. systematically assess impact...
Baker's yeast (Saccharomyces cerevisiae) has broad genetic homology to human cells. Although typically grown as 1-2mm diameter colonies under certain conditions can form very large (10 + mm in diameter) or 'giant' on agar. Giant have been used study diverse biomedical processes such cell survival, aging, and the response cancer pharmacogenomics. Such evolve dynamically into complex stratified structures that respond differentially environmental cues. Ammonia production, gravity driven...
Abstract Drug resistance is a consequence of how most modern medicines work. Drugs exert pressure on cells that causes death or the evolution resistance. Indeed, highly specific drugs are rendered ineffective by single DNA mutation. In this study, we apply drug methotrexate, which widely used in cancer and rheumatoid arthritis, perform experiments Baker’s yeast to ask different ways become resistant. Because conserved nature biological pathways between man, our results can inform same...
Lichen-forming fungi and extracts derived from them have been used as alternative medicine sources for millennia recently there has a renewed interest in their known bioactive properties anticancer agents, cosmetics antibiotics.Although lichen-forming fungus-derived compounds are biologically commercially valuable, few studies performed to determine modes of action.This study chemical-genetic chemogenomic high-throughput analyses gain insight into the action Caloplaca flavoruscens...