Alzheimer's disease (AD) is characterized by the deposition of amyloid plaques in parenchyma and vasculature brain. Although previous analytical studies have provided much information about composition structure synthetic amyloid-β fibrils, there is, surprisingly, a dearth data on intact from AD Therefore, to elucidate detailed isolated plaque cores, we utilized high-resolution, nondestructive technique, Raman microscopy. The are very high quality contain protein conformation,...

The application of Raman spectroscopy to characterize natively unfolded proteins has been underdeveloped, even though it significant technical advantages. We propose that a simple three-component band fitting the amide I region can assist in conformational characterization ensemble structures present proteins. spectra α-synuclein, prototypical protein, were obtained presence and absence methanol, sodium dodecyl sulfate (SDS), hexafluoro-2-propanol (HFIP). Consistent with previous CD studies,...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCrystal structure of a papain-E-64 complexK. I. Varughese, F. R. Ahmed, P. Carey, S. Hasnain, C. Huber, and A. StorerCite this: Biochemistry 1989, 28, 3, 1330–1332Publication Date (Print):February 7, 1989Publication History Published online1 May 2002Published inissue 7 February 1989https://doi.org/10.1021/bi00429a058Request reuse permissionsArticle Views624Altmetric-Citations123LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum...

Structural and electronic aspects of IrO2 films prepared by electrodeposition on Au substrates were investigated in situ LIII-edge X-ray absorption surface enhanced Raman spectroscopies both acid alkaline aqueous solutions. Linear correlations found between the extent oxidation Ir3+ determined from a statistical fit white line, which includes contributions each sites differing single electron, coulometric analysis voltammetric curves. Analysis extended fine structure (EXAFS) yielded Ir−O...

Detailed photostability studies were carried out using purified delta-endotoxin crystals from Bacillus thuringiensis subspecies HD-1 and HD-73. The mechanism time course of sunlight inactivation was investigated by: (a) monitoring the tryptophan damage in intact by Raman spectroscopy, (b) amino acid analysis (c) biological assays insects. results demonstrate that, for or HD-73 crystals, 300-380 nm range solar spectrum is largely responsible bringing about crystal consequent loss toxicity....

Trypsin is shown to generate an insecticidal toxin from the 130‐kDa protoxin of Bacillus thuringiensis subsp. kurstaki HD‐73 by unusual proteolytic process. Seven specific cleavages are occur in ordered sequence starting at C‐terminus and proceeding toward N‐terminal region. At each step, C‐terminal fragments approximately 10 kDa produced rapidly proteolyzed small peptides. The sequential proteolysis ends with a 67‐kDa which resistant further proteolysis. However, could be specifically split...

The hepatitis delta virus (HDV) ribozyme uses a cytosine to facilitate general acid−base catalysis. Biochemical studies suggest that C75 has pKa perturbed near neutrality. To measure this directly, Raman spectra were recorded on single crystals using microscope. A spectral feature arising from neutral was identified at 1528 cm-1. At low pH, mode replaced with new feature. Monitoring these features as function of pH revealed values for the couple anticooperatively Mg2+ binding, 6.15 and 6.40...

Abstract The carotenoids astaxanthin, lutein and zeaxanthin form high molecular weight aggregates in aqueous solvent systems. absorption spectra of the aggregates, compared to those monomer molecules, show significant bandwidth narrowing blue shifts 600–800 Å. resonance Raman these aggregated polyenes are anomalous that ν CC occurs near 1518 cm −1 whereas a well‐established vs 1/λ max correlation predicts should be 1560 . data together demonstrate strong excited state interactions taking...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTResonance Raman spectra of "blue" copper proteins and the nature their sitesOlavi Siiman, N. Martin Young, Paul R. CareyCite this: J. Am. Chem. Soc. 1976, 98, 3, 744–748Publication Date (Print):February 1, 1976Publication History Published online1 May 2002Published inissue 1 February 1976https://doi.org/10.1021/ja00419a017RIGHTS & PERMISSIONSArticle Views349Altmetric-Citations77LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full...

Bacillus thuringiensis produces a 130-140 kDa insecticidal protein in the form of bipyramidal crystal. The crystals from subspecies kurstaki HD-1 and entomocidus was found to contain 16-18 cysteine residues per molecule, present primarily disulphide as cystine. Evidence that all symmetrical interchain linkages crystal obtained following results: (i) diagonal procedure [Brown & Hartley (1966) Biochem. J. 101, 214-228] gave only unpaired cysteic acid peptides maps; (ii) bridges were shown...

Systemic amyloidoses, an important class of protein misfolding diseases, are often due to fibrillation disulfide-cross-linked globular proteins otherwise unrelated in sequence or structure. Although cross-beta assembly is regarded as a universal property polypeptides, it not understood how such amyloids accommodate diverse disulfide connectivities. Does amyloidogenicity depend on topology? A model provided by insulin, two-chain containing three bridges. The importance chain topology...

We report a simple three-step method of generating homogeneous toxic fragment (toxin) in high yield from B. thuringiensis var. kurstaki. Purified crystals were digested with trypsin at pH 10.5, followed by (NH4)2SO4 precipitation and dialysis. For the HD73 strain preparation is to eastern-spruce-budworm (Choristoneura fuminiferana) larvae. It gives single 66 kDa band on polyacrylamide-gel electrophoresis an isoelectric point 5.5 isoelectric-focusing gel. A isoleucine N-terminus was detected,...

Resonance Raman spectra are reported for several biologically important nitroanilines and nitrophenols. The data provide new insight into intramolecular interactions in highly conjugated nitroaromatics. In certain ortho substituted compounds the evidence structural changes going from ground to excited electronic state, or states, probed by resonance experiment. each compound bands show variation of intensity with excitation wavelength. For N-methyl-o- nitroaniline profiles some isolated...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTResonance Raman studies of blue copper proteinsOlavi Siiman, N. Martin Young, and Paul R. CareyCite this: J. Am. Chem. Soc. 1974, 96, 17, 5583–5585Publication Date (Print):August 1, 1974Publication History Published online1 May 2002Published inissue 1 August 1974https://pubs.acs.org/doi/10.1021/ja00824a053https://doi.org/10.1021/ja00824a053research-articleACS PublicationsRequest reuse permissionsArticle Views138Altmetric-Citations39LEARN ABOUT...