A5027667196- Cellular transport and secretion
- Protein Degradation and Inhibitors
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
University of Notre Dame
2019-2020
Abstract Breast cancer brain metastases (BCBM) have a 5-20 year latency and account for 30% of mortality; however, mechanisms governing adaptation to the microenvironment remain poorly defined. We combine time-course RNA-sequencing BCBM development with Drosophila melanogaster genetic screen, identify Rab11b as functional mediator metastatic adaptation. Proteomic analysis reveals that controls cell surface proteome, recycling proteins required successful interaction microenvironment,...
GRAPHICAL ABSTRACT SUMMARY Breast cancer brain metastases (BCBM) have a 5-20 year latency and account for up to 30% of mortality. Developing new therapeutics requires molecular understanding adaptation the microenvironment. Here, we combined RNA-sequencing BCBM development with reverse genetic screen in Drosophila melanogaster identified Rab11b, an endosomal recycling protein, as mediator metastatic adaptation. We show that disseminated cells up-regulate Rab11b early after arrival brain,...