• The differential accumulation of various polyphenols, particularly flavonoids and hydroxycinnamates, was studied in leaves Ligustrum vulgare exposed to increasing sunlight under well watered or drought-stress conditions. Light- drought-induced changes leaf polyphenol concentrations were normalized the CO2 assimilation rate. functional roles hydroxycinnamates analysed through tissue localization using multispectral fluorescence microimaging, efficiencies scavenge superoxide radicals (O2−)...

The production of olive oil yields a considerable amount waste water, which is powerful pollutant and currently discarded. Polyphenols other natural antioxidants, extracted from olives during extraction process, partially end up in the waters. Experimental commercial waters four Mediterranean countries were analyzed for possible recovering these biologically interesting constituents. Identification quantitation main polyphenols carried out by applying HPLC-DAD HPLC-MS methods. Representative...

In this study polyphenolic compounds extracted from olive fruits of five registered cultivars were analyzed. A solid−liquid extraction (LSE) procedure with Extrelut cartridge (diatomaceous earth) using different eluents was developed to obtain compounds. HPLC-DAD and HPLC-MS methods applied for the quali-quantitative analysis each fraction obtained LSE. The results work show that LSE diatomaceous earth supplies a rapid reproducible fractioning method able quantitative recovery all collect...

• The role of flavonoids in mechanisms acclimation to high solar radiation was analysed Ligustrum vulgare and Phillyrea latifolia, two Mediterranean shrubs that have the same flavonoid composition but differ strikingly their leaf morpho-anatomical traits. In plants exposed 12 or 100% radiation, measurements were made for surface morphology anatomy; optical properties, photosynthetic pigments, photosystem II efficiency; antioxidant enzymes, lipid peroxidation phenylalanine ammonia lyase;...

The antioxidant properties of galloyl quinic derivatives isolated from Pistacia lentiscus L. leaves have been investigated by means Electron Paramagnetic Resonance spectroscopy (EPR) and UV-Vis spectrophotometry. Antioxidant also estimated using the biologically relevant LDL test. scavenger activities gallic acid, 5-O-galloyl, 3,5-O-digalloyl, 3,4,5-O-trigalloyl acid derivatives, against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide (O2) hydroxyl (OH) radical. On whole, activity...

In the present study phenolic composition of leaves, stalks, and textile fiber extracts from Urtica dioica L. is described. Taking into account increasing demand for products made natural fibers necessity to create sustainable "local" processing chains, an Italian project was funded evaluate cultivation nettle in region Tuscany. The leaves two samples, cultivated wild (C W), contain large amounts chlorogenic 2-O-caffeoylmalic acid, which represent 71.5 76.5% total phenolics, respectively....

Oxidative stress is involved in the pathogenesis of numerous diseases. Nevertheless, no optimal natural antioxidant has been found for therapeutics, therefore polyphenol antioxidants have looked myrtle leaves, a plant that folk medicine used as anti-inflammatory drug. Antioxidant-rich fractions were prepared from (Myrtus communis L.) leaves liquid-liquid extraction (LLE) with different solvents. All extracts very rich polyphenols. In particular, hydroalcoholic contain galloyl-glucosides,...

Abstract Separation, identification and quantification of polyphenols was carried out on leaves Pistacia lentiscus L., an evergreen member the family Anacardiaceae, using semi‐preparative HPLC, HPLC‐photodiode array detection HPLC‐MS analysis, together with 1 H‐ 13 C NMR. Three major classes secondary metabolites were detected: (i) gallic acid galloyl derivatives both glucose quinic acid; (ii) flavonol glycosides, i.e. myricetin quercetin glycosides; (iii) anthocyanins, namely delphinidin 3‐...

• Salt- and light-induced changes in morpho-anatomical, physiological biochemical traits were analysed Myrtus communis Pistacia lentiscus with a view to explaining their ecological distribution the Mediterranean basin. In plants exposed 20 or 100% solar radiation supplied 0 200 mm NaCl, measurements conducted for ionic water relations photosynthetic performance, leaf morpho-anatomical optical properties tissue-specific accumulation of tannins flavonoids. Net carbon gain photosystem II (PSII)...

This paper describes a circular economy process based on environmentally and economically sustainable procedures which was applied to the sector of olive oil processing an industrial scale. Olea europaea L. tissues by-products represent renewable low-cost source polyphenols, in particular hydroxytyrosol (HTyr), naturally occurring compound well known for its biological properties. Specifically, green leaves (GL), dried (DL), pitted pulp were treated with water pneumatic extractor obtain...

A hydroxytyrosol (HTyr)-enriched fraction containing HTyr 6% w/w, derived from Olea europaea L. byproducts and obtained using an environmentally economically sustainable technology, was lipophilized under green chemistry conditions. The effects of three fractions hydroxytyrosyl butanoate, octanoate, oleate, named, respectively, lipophilic 5, 6, 7, unreacted on the human colon cancer cell line HCT8-β8 engineered to overexpress estrogen receptor β (ERβ) were evaluated compared those pure HTyr....

Sweet Chestnut ( Castanea sativa Mill.) wood extracts, rich in Hydrolyzable Tannins (HTs), are traditionally used the tanning and textile industries, but recent studies suggest additional uses. The aim of this work is HPLC-DAD-ESI-MS characterization aqueous extracts fractions obtained through a membrane separation technology system without using other solvents, evaluation their antioxidant antiradical activities. Total tannins range between 2.7 138.4 mM; gallic acid ranges 6% 100%;...

This study investigated the polyphenolic composition and antioxidant potential of Medjool date tissues (flesh seeds) from two plantations in Jordan Valley, using chromatographic (HPLC/DAD) spectrophotometric methods (Folin-Ciocalteau DPPH assays). Significant levels phenolic compounds, including gallic acid, ferulic tannins, flavonoids, were identified. The analysis revealed that polyphenol concentrations pulp varied depending on plantation practices environmental conditions. Additionally,...

Polyphenols are widespread in vegetables and fruits. They can play an important role human diet health, they influence the sensorial properties of many foods, act as natural antioxidants. This study was conducted using HPLC/DAD, tyrosinase biosensor, differential pulse voltammetry (DPV) analyses to detect polyphenolic compounds complex matrices. The were applied a series both standards extracts derived from grape, olives, green tea. pure include phenolic acids, flavones, flavonols,...

Anthocyanins (Anths) in olive (Olea europaea L.) fruits at different degrees of pigmentation were assessed nondestructively by measuring chlorophyll fluorescence (ChlF). The method is based on the comparison ChlF excitation spectra from olives with green to green−red, reddish−purple, and purple. logarithm ratio between (logFER) two colored zones gave difference absorption spectrum them. absorbance derived logFER a red same devoid skin showed typical Anth band (at 550 nm). It matched that...