A5045233115- Plant tissue culture and regeneration
- Insect Utilization and Effects
- Algal biology and biofuel production
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Plant Pathogens and Fungal Diseases
- Plant-Derived Bioactive Compounds
- Insect and Arachnid Ecology and Behavior
- Microbial Natural Products and Biosynthesis
- Bioenergy crop production and management
- Plant and animal studies
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Insect Pest Control Strategies
- Genetically Modified Organisms Research
- Potato Plant Research
- Polysaccharides and Plant Cell Walls
- Insect and Pesticide Research
- Plant Reproductive Biology
- Microbial Metabolites in Food Biotechnology
- Invertebrate Immune Response Mechanisms
- Plant Micronutrient Interactions and Effects
- Spatial and Panel Data Analysis
- Chromosomal and Genetic Variations
- Plant biochemistry and biosynthesis
Northeast Forestry University
2007-2024
Inner Mongolia Agricultural University
2014
The insect gut microbiota has been shown to contribute the host's digestion, detoxification, development, pathogen resistance, and physiology. However, there is poor information about ranking of these roles. Most results were obtained with cultivable bacteria, whereas bacterial physiology may be different between free-living midgut-colonizing bacteria. In this study, we provided both proteomic genomic evidence on roles bacteria by investigating anal droplets from a weevil, Cryptorhynchus...
The community of endophytic fungi associated with Acer ginnala, a common tree in northeastern China, was investigated. Four media, PDA, Czapek’s, WA and Sabouraud’s, were used to inoculate explants from seeds, annual twigs perennial (xylem bark). Media strongly affected the isolated species number, but not colonization frequency (CF) or isolation (IF). To investigate media effect further, Principal Component Analysis (PCA) done. As result, two components accounted for 86.502% total variance...
Exopolysaccharides produced by edible microorganisms exhibit excellent constructive physicochemical and significant biological activity, which provide advantages for the food or pharmaceutical industries. Two novel exopolysaccharides Debaryomyces hansenii DH-1 were characterized, named S1 S2, respectively. S1, with a molecular weight of 34.594 kDa, primarily consisted mannose glucose in molar ratio 12.19:1.00, contained backbone fragment...
Insects share similar fundamental molecular principles with mammals in innate immunity. For modulating normal gut microbiota, insects produce phenoloxidase (PO), which is absent all vertebrates, and reactive nitrogen species (ROS) antimicrobial proteins (AMPs). However, reports on insect phagocytosis are very few. Furthermore, most previous studies measure gene expression at the transcription level. In this study, we provided proteomic evidence modulation of microorganisms by investigating...
Study on the characteristics of integration and expression is basis genetic stability foreign genes in transgenic trees. To obtain insight into relationship transgene copy number level, we screened 22 birch lines. Southern blot analysis plants indicated that bgt varies from one to four, which 18.2% were single copy. Copy gus was different 68% plants, indicating rearrangement or partial deletion appeared process T-DNA integration. Transcriptional gene analyzed by Northern blot. TGS has been...
The spatial pattern of diseased forest trees is a product the host and disease itself. Previous studies have focused on describing trees, it remains largely unknown whether an antecedent host/nonhost affects infection how large effect sizes size are. results from trivariate random labeling showed that ash tree, Fraxinus mandshurica, but not nonhost tree species, impacted stem fungal caused by Inonotus hispidus. To investigate we employed SADIE (Spatial Analysis Distance IndicEs) aggregation...
Fraxinus mandshurica Rupr. ( F . ) is a dioecious tree species with important ecological and application values. To delve deeper into the regulatory pathways genes responsible for male female flowers in , we conducted transcriptome sequencing on at four distinct stages. The analysis revealed that database generated 38,319,967 reads while 43,320,907 reads, resulting 2930 differentially expressed 1441 were up-regulated 1489 down-regulated males compared to females. Following an of Gene...
Abstract Background: Manchurian ash ( Fraxinus mandshurica Rupr.) is an endangered hardwood tree species, providing both economic and medicinal benefits. However, observations such as browning of adventitious shoot buds high rate somatic embryo abnormality, were presented in protocols F. regeneration. Therefore, a rapid high-efficiency regeneration system demanded for mass propagation genetic transformation . Results: We have developed efficient through organogenesis using hypocotyl...
In this paper, Southern blot analysis of the transgenic birch (Betula platyphylla Suk.) plants indicated that copy number bgt varies from one to four. Of which 18.2 percent were single copy. Transcriptional expression gene in was analyzed by Northern blot. TGS has been found four lines with one, three and copies bgt. Bgt inserted into prokaryotic vector pET 28a transferred E.coli BL21 (DE3) for BGT fusion protein. After overnight cleavage Thrombin purification immobilized metal ion affinity...