A5059615221- Trypanosoma species research and implications
- Toxoplasma gondii Research Studies
- RNA and protein synthesis mechanisms
- Lysosomal Storage Disorders Research
- DNA and Nucleic Acid Chemistry
- Ion channel regulation and function
- Cardiomyopathy and Myosin Studies
- Mosquito-borne diseases and control
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Calcium signaling and nucleotide metabolism
- Synthesis and Characterization of Heterocyclic Compounds
- Macrophage Migration Inhibitory Factor
- Synthesis of heterocyclic compounds
- Malaria Research and Control
- Genetic Neurodegenerative Diseases
- Cellular transport and secretion
- Cardiovascular Effects of Exercise
- Protein Structure and Dynamics
University of Vienna
2019-2023
Max Perutz Labs
2019-2023
Vienna Biocenter
2019-2023
University of Würzburg
2022
University of Ljubljana
2019
Medical University of Vienna
2019
MORN (Membrane Occupation and Recognition Nexus) repeat proteins have a wide taxonomic distribution, being found in both prokaryotes eukaryotes. Despite this ubiquity, they remain poorly characterised at structural functional level compared to other common repeats. In terms, are often assumed be lipid-binding modules that mediate membrane targeting. We addressed putative activity by focusing on protein composed solely of repeats— Trypanosoma brucei MORN1. Surprisingly, no evidence for...
MIF is a ubiquitous protein involved in proinflammatory processes, which undergoes an oxidation-driven conformational change to oxidized (ox)MIF. We demonstrate that hypochlorous acid, produced by neutrophil-released myeloperoxidase (MPO) under inflammatory conditions, effectively oxidizes into the oxMIF isoform, specifically recognized anti-oxMIF therapeutic antibody, ON104. NMR investigation of MPO system revealed increased flexibility throughout structure, including at several catalytic...
Sarcomeres, the basic contractile units of striated muscle cells, contain arrays thin (actin) and thick (myosin) filaments that slide past each other during contraction. The Ig-like domain-containing protein myotilin provides structural integrity to Z-discs—the boundaries between adjacent sarcomeres. Myotilin binds Z-disc components, including F-actin α-actinin-2, but molecular mechanism binding implications these interactions on are still elusive. To illuminate them, we used a combination...
ABSTRACT MORN (Membrane Occupation and Recognition Nexus) repeat proteins have a wide taxonomic distribution, being found in both prokaryotes eukaryotes. Despite this ubiquity, they remain poorly characterised at structural functional level compared to other common repeats. In terms, are often assumed be lipid-binding modules that mediate membrane targeting. We addressed putative activity by focusing on protein composed solely of repeats – Trypanosoma brucei MORN1. Surprisingly, no evidence...
ABSTRACT All endocytosis and exocytosis in the African trypanosome Trypanosoma brucei occurs at a single subdomain of plasma membrane. This subdomain, flagellar pocket, is small vase-shaped invagination containing root flagellum cell. Several cytoskeleton-associated multiprotein complexes are coiled around neck pocket on its cytoplasmic face. One these, hook complex, was proposed to affect macromolecule entry into lumen. In previous work, knockdown T. (Tb)MORN1, complex component, resulted...
Abstract All endo- and exocytosis in the African trypanosome Trypanosoma brucei occurs at a single subdomain of plasma membrane. This subdomain, flagellar pocket, is small vase-shaped invagination containing root cell’s flagellum. Several cytoskeleton-associated multiprotein complexes are coiled around neck pocket on its cytoplasmic face. One these, hook complex, was proposed to affect macromolecule entry into lumen. In previous work, knockdown complex component TbMORN1 resulted larger cargo...
ABSTRACT Measuring and quantifying thermodynamic parameters that determine stability of interactions between biological macromolecules is an essential necessary complement to structural studies. Although several laboratories are able obtain basic for the observed process, data interpretation analysis quality reported can be extremely tedious. We have started develop a web application will help users perform characterization G-quadruplex unfolding. The global fitting calorimetric...
Measuring and quantifying thermodynamic parameters that determine both the stability of interactions between biological macromolecules are an essential necessary complement to structural studies. Although basic for observed process can be readily obtained, data interpretation is often slow analysis quality extremely variable. We have started develop a web application will help users perform characterizations oligonucleotide unfolding. The global fitting calorimetric spectroscopic data, uses...
Abstract Sarcomeres, the basic contractile units of striated muscle cells, contain arrays thin (actin) and thick (myosin) filaments that slide past each other during contraction. The Ig-like domain containing protein myotilin provides structural integrity to Z-discs - boundaries between adjacent sarcomeres. Myotilin binds Z-disc components, including F-actin α-actinin-2, but molecular mechanism binding implications these interactions on are still elusive. We used a combination small angle...