A5039795791- Protein purification and stability
- Analytical chemistry methods development
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Chromium effects and bioremediation
- Ion-surface interactions and analysis
- Radioactive element chemistry and processing
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Gold and Silver Nanoparticles Synthesis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Geochemistry and Elemental Analysis
- Electrochemical sensors and biosensors
- Extracellular vesicles in disease
- Nuclear Materials and Properties
- Nuclear Physics and Applications
Clemson University
2022-2024
Oak Ridge National Laboratory
2020
The determination of trace elements, particularly rare earth in uranium ore concentrates (UOCs) is important as the pattern can be indictive characteristics. Presented here a methodology for accurately quantifying elements (REE) UOCs. To improve measurement uncertainty, isotope dilution mass spectrometry (IDMS) was utilized over other quantification techniques such external calibration or standard addition. isotopic determinations were measured by inductively coupled plasma-mass (ICP-MS)....
Extracellular vesicles (EVs) have garnered much interest due to their fundamental role in intracellular communication and potential utility clinical diagnostics as biotherapeutic vectors. Of particular relevance is the subset of EVs referred exosomes, ranging size from 30 150 nm, which contain incredible amounts information about cell origin, can be used track progress disease. As a complementary action, exosomes engineered with therapeutic cargo selectively target diseases. At present, lack...
Cell culture media (CCM) contain multiple metal nutrients in diverse chemical forms. A rapid method is developed to perform first-level (inorganic vs. ligated) speciation employing a novel polymer fiber chromatography column and ICP-MS.
Cultured cell lines are very commonly used for the mass production of therapeutic proteins, such as monoclonal antibodies (mAbs). In particular, Chinese hamster ovary (CHO) widely employed due to their high tolerance variations in experimental conditions and ability grow suspension or serum free media. CHO known produce titers biotherapeutic products immunoglobulin G (IgG). An emergent alternative means treating diseases, cancer, is use gene therapies, wherein genetic cargo "packaged"...
Cell culture media metal content is critical in mammalian cell growth and monoclonal antibody productivity. The variability concentrations has multiple sources of origin. As such, there a need to analyze before, during, after production. Furthermore, it not the simple presence given that can impact processes, but also their chemical form is, speciation. To first approximation, instructive simply quickly ascertain if metals exist as inorganic (free metal) ions or are part an organometallic...
Presented here is a novel automated method for determining the trace element composition of bulk thorium by inductively coupled plasma–optical emission spectroscopy (ICP-OES). ICP-OES universal approach measuring elemental impurities present in actinide-rich materials; however, due to rich spectrum actinide, separation from elements warranted spectrochemical analysis. Here, AG MP-1 ion exchange resin was utilized retention Th matrix, while allowing be separated prior subsequent analysis...