A5101668674- Viral Infectious Diseases and Gene Expression in Insects
- Educational Innovations and Technology
- Business, Innovation, and Economy
- E-Learning and Knowledge Management
- Regional Development and Innovation
- Virus-based gene therapy research
- Organizational Management and Innovation
- 3D Printing in Biomedical Research
- Education and Teacher Training
- Digital literacy in education
- CRISPR and Genetic Engineering
- Higher Education Teaching and Evaluation
- Online and Blended Learning
- Educational theories and practices
- Online Learning and Analytics
- Higher Education and Sustainability
- Business, Education, Mathematics Research
- Environmental and Ecological Studies
- Diverse Applied Research Studies
- Technology in Education and Healthcare
- Crystallization and Solubility Studies
- Knowledge Societies in the 21st Century
- Consumer Retail Behavior Studies
- Educational Innovations and Challenges
- Literacy and Educational Practices
Universidad Autónoma de Nuevo León
2015-2024
Laboratoire de Recherche Scientifique
2021
Universidad Autónoma de Madrid
2019
Impact
2017
Universidad Panamericana de Nuevo Laredo
2017
Fundación Universitaria San Pablo CEU
2017
Universidade de Santiago de Compostela
2016
Universidad Politécnica de Madrid
2010
Bioscience (China)
2007
Integrative Medicine Institute
2007
Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient are low titers and specific productivity compared to stable cell lines. Here, we report an improved protocol that yields exceeding 1 g/l HEK293E cells. This was achieved by combining a new highly efficient polyethyleneimine (PEI)-based protocol, optimized gene expression vectors, use cycle regulators p18 p21, acidic Fibroblast Growth Factor, exposure cells valproic acid...
Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins still more than an order of magnitude lower yields typically achieved with cell lines. Here protein production Chinese hamster ovary transfected linear 25 kDa polyethylenimine was significantly enhanced by incubation at temperatures ranging from 29 33 degrees C after DNA delivery. With this approach, antibody 60-80 mg/L were...
En 2020, a raíz de la COVID-19, Perú experimentó un rápido crecimiento en implementación tecnología Big Data. El objetivo este estudio es determinar influencia del conocimiento Data sobre el desempeño financiero las empresas peruanas, mediado por capacidad gestión. Para ello, se empleó enfoque cuantitativo correlacional con diseño no experimental, que participaron 77 gerentes grandes peruanas incluidas ranking mejores empresas. La técnica análisis utilizada fue modelo ecuaciones...
Orbitally shaken cylindrical bioreactors [OrbShake (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50‐mL tubes with a ventilated cap (OSR50), originally derived from standard laboratory centrifuge conical bottom, have found many applications including high‐throughput screening optimization cell conditions. To better understand fluid dynamics and gas transfer rates at liquid surface in OSR50, we...
We present a new approach for biomass assessment in cell culture using disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber sample loading and lower 5 µL capillary collection during centrifugation. resulting packed volume (PCV) can be quantitatively expressed as the percentage of total sample. study focused on validation method mammalian lines that are widely used bioprocessing. Using several examples, PCV was shown to more precise, rapid, reproducible...
Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce backwards and forwards 'wave' , or orbital shaking generate that runs round cylindrical container, have proved be successful suspension cultures cells, especially when combined with disposable materials. This article presents an overview engineering characteristics these new are applied case studies covering scales operation from milliliters 1000 liters.
There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37 degrees C and freezing in small batches. We investigated a third approach, the "pausing" of cells days or weeks temperatures below variety cultivation vessels. High cell viability exponential growth were observed after pausing recombinant Chinese hamster ovary line (CHO-Clone 161) temperature range 6-24 microcentrifuge tubes up to 3 weeks. After T-flasks 4 9 days, adherent cultures CHO-DG44...
Facebook is essentially a successful online social network site, in which individuals can share information; the company was founded 2004 by Mark Zuckerberg, Dustin Moskovitz and Chris Hughes as way to support communication of Harvard University. This used within community, then quickly spread out other institutions. Site opened public 2006. Nine years later, when established it became into most popular world, more users are being added every day, coming already be millions around including...
Transient gene expression (TGE) is the most commonly used technology for rapid production of moderate quantities recombinant proteins preclinical studies or analytical assay development [1,2]. Whereas TGE can provide milligram to gram amounts within a time period as short few days, technical limitations and high costs plasmid DNA hamper application this at larger scales. Here we describe an alternative method fast from pools mammalian cells stably transfected with PiggyBac (PB) transposon....
This study presents the background of theoretical models culture shock and adaptation in international students higher education. The Stress Stressors that might be experience by these is measured Perceives Scale 10-Item version Focus Groups Interviews to Faculty Staff Members a Mexican Private University. Guidelines procedures Counselors are shared as well results data obtained this Mixed-Method Approach Study.
Abstract: This study aimed to evaluate the quality of university services in business school at a public Mexico from perspective its actors (students and teachers). To get their results, SERVQUAL scale was used with some adjustments, which is an instrument multiple items that were originally developed measure customer insights regarding service. The consists 22 phrases expectations similar perception service established by difference scores assigns perceptions.Questions serve other aspects...
ResumenEn este trabajo se analizó la influencia que inclusión de castaña en formulación piensos ejerce sobre calidad canal y carne 24 cerdos industriales.La tanto a un 15% (C15) como 25% (C25) incrementaron el peso vivo (140 kg vs. 128 kg) (en torno 114 103 comparación con control.En cuanto las medidas solo longitud mostró diferencias entre los grupos.En grupo C25 fueron superiores rendimiento (82 80% otros) pesos porcentajes lomo jamón, mientras resto piezas no observaron significativas.En...
A major bottleneck in the manufacture of recombinant therapeutic proteins is time and effort needed for generation stable, high-producing mammalian cell lines. Conventional gene transfer methods stable line rely on random transgene integration, resulting unpredictable highly variable levels expression individual clones [1,2]. As a consequence, large number stably transfected cells must be analyzed to recover few clones. Recently, we described use PiggyBac (PB) transposon lines [3]. The PB...
Appears in: INTED2024 Proceedings Publication year: 2024Pages: 5197-5204ISBN: 978-84-09-59215-9ISSN: 2340-1079doi: 10.21125/inted.2024.1346Conference name: 18th International Technology, Education and Development ConferenceDates: 4-6 March, 2024Location: Valencia, Spain