A5075494402- Signaling Pathways in Disease
- Cardiac Ischemia and Reperfusion
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Cardiac Fibrosis and Remodeling
- Hormonal Regulation and Hypertension
- Nitric Oxide and Endothelin Effects
- Cardiac electrophysiology and arrhythmias
- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- Eicosanoids and Hypertension Pharmacology
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
SickKids Foundation
2024
University of Toronto
2024
Hospital for Sick Children
2021
University of Alberta
2019
Significance The HtrA2 protease plays a critical role in ensuring proper cellular function and regulating variety of different processes, including programmed cell death. Although the three-dimensional structure trimeric form enzyme has been determined, mechanism by which this important molecule is regulated remains to be elucidated. Our data reveal that accomplished through second conformation it forms hexamer with much lower affinity for substrate fact low-activity state until binding...
Many challenges remain in the preclinical evaluation, adjudication, and prioritization of novel compounds therapeutic discovery pipelines. These obstacles are evident by large number candidate or lead failing to reach clinical trials, significantly due a lack efficacy disease paradigm interest and/or presence innate chemical toxicity. The consequential compound attrition pipelines results added monetary time costs, potential danger patients, slowed true therapeutics. low rate successful...
Lipopolysaccharide (LPS) is a bacterial wall endotoxin producing many pathophysiological conditions including myocardial inflammation leading to cardiotoxicity. Linoleic acid (18:2n6, LA) an essential n-6 PUFA which converted arachidonic (20:4n6, AA) by desaturation and elongation via enzyme systems within the body. Biological transformation of through CYP-mediated hydroxylation, epoxidation allylic oxidation produces lipid mediators, may be subsequently hydrolyzed corresponding diol...
Matrix metalloproteinase-2 (MMP-2) is a zinc-dependent protease which contributes to cardiac contractile dysfunction when activated during myocardial ischaemia-reperfusion (IR) injury. MMP-2 localized several subcellular sites inside myocytes; however, its role in the sarcoplasmic reticulum (SR) unknown. The Ca2+ ATPase SERCA2a, pumps cytosolic into SR facilitate muscle relaxation, degraded IR injury; responsible for this unclear. We hypothesized that by proteolyzing thereby impairing...