A5027260863- Microbial metabolism and enzyme function
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Alcohol Consumption and Health Effects
- 3D Printing in Biomedical Research
- Metalloenzymes and iron-sulfur proteins
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Fungal and yeast genetics research
- Gut microbiota and health
- Fungal Biology and Applications
- Genomics and Phylogenetic Studies
- Microbial Fuel Cells and Bioremediation
- Metal-Catalyzed Oxygenation Mechanisms
- Wastewater Treatment and Nitrogen Removal
- Porphyrin Metabolism and Disorders
- Biochemical and biochemical processes
- Pharmacological Effects of Natural Compounds
- Biochemical Acid Research Studies
- Pickering emulsions and particle stabilization
Okayama University
2015-2023
Metabolomic analysis revealed that Methylobacterium cells accumulate a large amount of ergothioneine (EGT), which is sulfur-containing, non-proteinogenic, antioxidative amino acid derived from histidine. EGT biosynthesis and its role in methylotrophy physiology for plant surface-symbiotic species were investigated this study. Almost all type strains can synthesize EGT. We selected one the most productive (M. aquaticum strain 22A isolated moss), feasibility fermentative production through...
Methylobacterium species are representative of methylotrophic bacteria. Their genomes usually encode two types methanol dehydrogenases (MDHs): MxaF and XoxF. The former is a Ca2+-dependent enzyme, the latter was recently determined to be lanthanide-dependent enzyme that necessary for expression mxaF. This finding revealed unexpected important roles lanthanides in bacterial methylotrophy. In this study, we performed transcriptome sequencing (RNA-seq) analysis using M. aquaticum strain 22A...
Methylobacterium species frequently inhabit plant surfaces and are able to utilize the methanol emitted from plants as carbon energy sources. As some of known promote growth, significant attention has been paid mechanism growth promotion specificity plant–microbe interactions. By screening our isolate collection for high effect in vitro, we selected candidates field pot tests rice barley, respectively. We found that inoculation resulted better ripening seeds, increased size barley grains but...
The pink-pigmented facultative methylotrophs (PPFMs), a major bacterial group found in the plant phyllosphere, comprise two genera: Methylobacterium and Methylorubrum. They have been separated into three clades: A, B (Methylorubrum), C. Within these genera, however, some species lack either pigmentation or methylotrophy, which raises question of what actually defines PPFMs. present study employed comprehensive comparative genomics approach to reveal phylogenetic relationship among PPFMs...
Methylobacterium and Methylorubrum species are facultative methylotrophic bacteria that abundant in the plant phyllosphere. They have two methanol dehydrogenases, MxaF XoxF, which dependent on either calcium or lanthanides (Lns), respectively. Lns exist as insoluble minerals nature, their solubilization uptake require a siderophore-like substance (lanthanophore). also been identified growth-promoting although actual mechanism has not well-investigated. This study aimed to reveal roles of...
A novel facultatively methanol-utilizing bacterial strain, SM30T, was isolated from rice rhizosphere. Strain SM30T Gram-stain-negative, aerobic, motile, short rods, and grew optimally at pH 7 28 °C. It could tolerate 0 to 2 % (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain most closely related Pleomorphomonas oryzae DSM 16300T, with a low similarity of 94.17 %. One the lanthanide metals, lanthanum, enhance its growth slightly methanol. Phylogenetic trees, based mxaF, xoxF...
Lanthanides (Ln) are an essential cofactor for XoxF-type methanol dehydrogenases (MDHs) in Gram-negative methylotrophs. The Ln3+ dependency of XoxF has expanded knowledge and raised new questions methylotrophy, including the differences characteristics MDHs, their regulation, methylotrophic metabolism formaldehyde oxidation. In this study, we genetically identified one set Ln3+- Ca2+-dependent MDHs (XoxF1 MxaFI), that involved ExaF-type Ln3+-dependent ethanol dehydrogenase, among six...
Methylobacterium and Methylorubrum species oxidize methanol via pyrroloquinoline quinone-methanol dehydrogenases (MDHs). MDHs can be classified into two major groups, Ca2+-dependent MDH (MxaF) lanthanide (Ln3+)-dependent (XoxF), whose expression is regulated by the availability of Ln3+. A set a siderophore, TonB-dependent receptor, an ABC transporter that resembles machinery for iron uptake involved in solubilization transport The Ln3+ cytosol enhances XoxF expression. unique protein named...
Motile bacteria often exhibit chemotaxis toward favorable compounds. However, the diversity of that are attracted to a given substance is largely unknown. This study aimed reveal with natural towards methanol. We tried enrich environmental chemotactic using glass capillary half-filled methanol solidified agarose as trap ("chemotaxis fishing"). The pilot experiment methanol-chemotactic Methylobacterium aquaticum strain 22A enriched cells by 46-fold. method was then applied bacterial...
Motile bacteria take a competitive advantage in colonization of plant surfaces to establish beneficial associations that eventually support health. Plant exudates serve not only as primary growth substrates for but also bacterial chemotaxis attractants. A number plant-derived compounds and corresponding sensors have been documented, however, the methanol, one major volatile released by plants, identified. Methylobacterium species are ubiquitous surface-symbiotic, methylotrophic bacteria....