A5081715353- Mass Spectrometry Techniques and Applications
- Analytical chemistry methods development
- Radioactive element chemistry and processing
- Metabolomics and Mass Spectrometry Studies
- Pesticide Residue Analysis and Safety
AstraZeneca (United Kingdom)
2022
Large compound libraries utilised for HTS often include metal contaminated compounds which can interfere with assay signal or target biology, and therefore appear as hits. Pursuit of these divert considerable time resource away from more propitious hits, yet there is currently no established method detecting impurities in a rapid effective manner. Here we describe the development application high-throughput to identify contaminants using acoustic mist ionisation mass spectrometry (AMI-MS)....
Rapid triage of compounds acting via undesired mechanisms is a crucial stage in high-throughput screening (HTS) cascade to ensure time and resource efficiently assigned the most propitious hits. Redox cycling (RCCs) produce reactive oxygen species, such as hydrogen peroxide, which can impair protein function appear hits against liable targets. Direct measurement tris(2-carboxyethyl)phosphine (TCEP) oxidation has been demonstrated sensitive accurate measure redox [1]. However, current nuclear...