A5032398566- Zebrafish Biomedical Research Applications
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Neuroscience and Neuropharmacology Research
- Nanoparticle-Based Drug Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Polymer Surface Interaction Studies
- Extracellular vesicles in disease
- Congenital Ear and Nasal Anomalies
- Parkinson's Disease Mechanisms and Treatments
- Muscle Physiology and Disorders
- Otitis Media and Relapsing Polychondritis
- Advanced Fluorescence Microscopy Techniques
- Prion Diseases and Protein Misfolding
- MicroRNA in disease regulation
- Tracheal and airway disorders
- Nanoplatforms for cancer theranostics
- Congenital heart defects research
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
- Sphingolipid Metabolism and Signaling
- Cholesterol and Lipid Metabolism
- Optical Coherence Tomography Applications
- Advanced Optical Sensing Technologies
Institut National de la Recherche Scientifique
2016-2025
Université de Montréal
2012-2025
Université du Québec
2024
Université du Québec à Montréal
2019-2024
Armand Frappier Museum
2016
Centre Hospitalier Universitaire Sainte-Justine
2012
University of Alberta
2005-2010
Introduction: Drug delivery across the blood-brain barrier (BBB) is challenging and therefore severely restricts neurodegenerative diseases therapy such as Alzheimer's disease (AD). Donepezil (DNZ) an acetylcholinesterase (AChE) inhibitor largely prescribed to AD patients, but its use limited due peripheral adverse events. Nanodelivery strategies with polymer Poly (lactic acid)-poly(ethylene glycol)-based nanoparticles (NPs-PLA-PEG) extracellular vesicles (EVs) were developed aim improve...
The DNA/RNA-binding proteins TDP-43 and FUS are found in protein aggregates a growing number of neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) related dementia, but little is known about the neurotoxic mechanisms. We have generated Caenorhabditis elegans zebrafish animal models expressing mutant human (A315T or G348C) (S57Δ R521H) that reflect certain aspects ALS motor neuron degeneration, axonal deficits, progressive paralysis. To explore potential our humanized...
CHARGE syndrome is caused by mutations in the CHD7 gene. Several organ systems including retina, cranial nerves, inner ear and heart are affected syndrome. However, mechanistic link between many of dysfunction remains elusive. Here, we show that Chd7 required for organization neural retina zebrafish. We observe an abnormal expression or a complete absence molecular markers retinal ganglion cells photoreceptors, indicating regulates differentiation plays essential role cell development. In...
Abstract The most common genetic cause of amyotrophic lateral sclerosis (ALS) and fronto-temporal dementia (FTD) is a hexanucleotide repeat expansion within the C9orf72 gene. Reduced levels mRNA protein have been found in ALS/FTD patients, but role this disease pathogenesis still poorly understood. Here, we report generation characterization stable loss-of-function (LOF) model zebrafish. We show that reduced function leads to motor defects, muscle atrophy, neuron loss mortality early larval...
One important challenge in treating avascular-degraded cartilage is the development of new drugs for both pain management and joint preservation. Considerable efforts have been invested developing nanosystems using biomaterials, such as chitosan, a widely used natural polymer exhibiting numerous advantages, i.e., non-toxic, biocompatible biodegradable. However, even if chitosan generally recognized safe, safety biocompatibility nanomaterials must be addressed because potential greater...
A new theoretical framework that enables the use of differential dynamic microscopy (DDM) in fluorescence imaging mode to quantify situ protein adsorption onto nanoparticles (NP) while simultaneously monitoring for NP aggregation is proposed. This methodology used elucidate thermodynamic and kinetic properties corona (PC) vitro vivo. The results show triggers particle over a wide concentration range formed aggregate structures can be quantified using proposed methodology. Protein affinity...
RNA polymerase III transcribes essential non-coding RNAs, a process regulated by transcription factors TFIIIB and TFIIIC. Although germline variants in TFIIIC subunit genes have been described few patients with neurodevelopmental disorders, the associated pathogenesis clinical spectrum are not yet well defined. Herein, we describe identification of biallelic GTF3C3, which encodes key component subunit, four from three unrelated families different ethnicities collected through GeneMatcher....
Abstract BLOC-one-related complex (BORC) is a multiprotein composed of eight subunits named BORCS1–8. BORC associates with the cytosolic face lysosomes, where it sequentially recruits small GTPase ARL8 and kinesin-1 -3 microtubule motors to promote anterograde transport lysosomes toward peripheral cytoplasm in non-neuronal cells distal axon neurons. The physiological pathological importance humans, however, remains be determined. Here, we report identification compound heterozygous variants...
Article26 April 2021Open Access Transparent process Chromatin remodeller CHD7 is required for GABAergic neuron development by promoting PAQR3 expression Priyanka Jamadagni INRS- Centre Armand-Frappier Santé Biotechnologie, Laval, QC, Canada Search more papers this author Maximilian Breuer Kathrin Schmeisser de recherche du Hospitalier l'Université Montréal (CRCHUM), Montréal, Tatiana Cardinal d'Excellence en Recherche sur les Maladies Orphelines - Fondation Courtois (CERMO-FC), Université...
Drug nanocarriers (NCs) capable of crossing the vascular endothelium and deeply penetrating into dense tissues CNS could potentially transform management neurological diseases. In present study, we investigated interaction bottle-brush (BB) polymers with different biological barriers in vitro vivo compared it to nanospheres similar composition. internalization permeability assays revealed that BB are not internalized by brain-associated cell lines translocate much faster across a blood–brain...
Abstract Protein kinase C isozymes are a biologically diverse group of enzymes known to be involved in wide variety cellular processes. They fall into three families (conventional, novel and atypical) depending upon their mode activation. Several classes zebrafish neurons have been shown express PKCα during development, but the expression other isoforms remains unknown. In this study we performed immunohistochemistry determine if various PKC. We used antibodies test for presence that thought...
The trafficking of AMPA receptors (Rs) to and from synaptic membranes is a key component underlying plasticity mechanisms such as long-term potentiation (LTP) depression (LTD), likely important for development in embryonic organisms. However, some the biochemical components required receptor embryos are still unknown. Here, we report that zebrafish, activation PKCgamma by phorbol 12-myristate 13-acetate, strongly potentiates amplitude AMPAR-mediated miniature excitatory postsynaptic currents...
Glutamate AMPA receptors (AMPARs) are major excitatory in the vertebrate CNS. In many biological systems there is a developmental speeding AMPAR kinetics, which occurs either because of switch subunits or change synaptic morphology. We studied development AMPAR-mediated miniature postsynaptic currents (AMPAR-mEPSCs) zebrafish Mauthner cells (M-cells) to determine reasons underlying mEPSCs this preparation. recorded AMPAR-mEPSCs ranging age from 33 h postfertilization (hpf) 72 hpf. found that...
Persistent reactive oxygen species (ROS) and neuroinflammation contribute to the onset progression of neurodegenerative diseases, underscoring need for targeted therapeutic strategies mitigate these effects. Extracellular vesicles (EVs) show promise in drug delivery due their biocompatibility, ability cross biological barriers, specific interactions with cell tissue receptors. In this study, we demonstrated that human plasma-derived EVs (pEVs) exhibit higher brain-targeting specificity,...
Neuromuscular junction (NMJ) structural integrity is crucial for transducing motor neuron signals that initiate skeletal muscle contraction. Zebrafish has emerged as a simple and efficient model to study NMJ morphology function in the context of developmental neurobiology neuromuscular diseases. However, methods quantify from voluminous data confocal images accurately, rapidly, reproducibly are lacking.We developed an ImageJ macro called "NMJ Analyser" automatically unbiasedly analyse...
Voltage-gated Na+ and K+ channels play key roles in the excitability of skeletal muscle fibers. In this study we investigated steady-state kinetic properties voltage-gated currents slow fast fibers zebrafish ranging age from 1 day postfertilization (dpf) to 4-6 dpf. The inner white (fast) possess an A-type inactivating current that increases peak density accelerates its rise decay times during development. As matured, V50s activation inactivation became more depolarized, then hyperpolarized...
Abstract A key step in the maturation of glutamate synapses is developmental speeding α‐amino‐3‐hydroxyl‐5‐methyl‐4‐isoxazole‐propionate receptor (AMPA‐R) kinetics, which occurs via a switch subtypes. However, molecular components required for receptors are unknown. Here, we used zebrafish preparation to show that activation protein kinase C (PKC)γ necessary AMPA‐R kinetics. Targeted knockdown PKCγ with an antisense morpholino oligonucleotide [PKCγ‐morpholino (PKCγ‐MO)], prevents normal up...
Oxysterols have essential effects on brain homeostasis and their levels are often altered in neurodegenerative neuroinflammatory diseases. Several studies demonstrated the cytotoxic of 25-HC different cell lines, however, not much is known about its neurons vivo. In this study, we examined exposure nervous system development zebrafish. We showed that survival rate zebrafish embryos/larvae significantly decreased at doses above 40 μM. was found to affect motility larvae, primary motor axon...
Conventional second harmonic generation (SHG) microscopy might not clearly reveal the structure of complex samples if interference between all scatterers in focal volume results artefactual patterns.We report here use interferometric (I-SHG) to efficiently remove these artifacts from SHG images.Interfaces two regions opposite polarity are considered because they known produce imaging muscle for instance.As a model system, such interfaces first studied periodically-poled lithium niobate...