A heterologous radioimmunoassay was used to measure the concentration of immunoreactive atrial natriuretic peptide (iANP) in plasma from femoral artery eight chloralose anaesthetized dogs. Mitral obstruction which increased left pressure by 11 cmH 2 O iANP 97 ± 10.3 (mean SE) 135 14.3 pg/mL. Pulmonary vein distension heart rate but did not increase iANP. Bilateral cervical vagotomy and administration atenolol (2 mg/kg) prevent with mitral obstruction. Samples blood coronary sinus had...

The distribution of atrial natriuretic factor (ANF) binding sites in Wistar rat tissues, as well tissues from other species was studied. Using autoradiography slide mounted tissue sections incubated with 125 I-labelled ANF, high densities were found the renal glomeruli and papilla, aortic smooth muscle, iliac vein, choroid plexus, anterior pituitary, lung, adrenal zona glomerulosa. Results renal, aortic, adrenal, lung spontaneously hypertensive rats did not differ those rats. Binding also...

In 12 chloralose anaesthetized dogs plasma concentration of immunoreactive atrial natriuretic peptide (IR-ANP) was measured using a radioimmunoassay. Plasma IR-ANP 74 ± 4.8 pg/mL (mean SE) and increased by 39 4.1 when left pressure 10 cm H 2 O during partial mitral obstruction. Observation the time course changes in distension showed that within min declined after distension, with half-time 4.5 min. The unaffected vagotomy or administration atenolol. Maximum electrical stimulation right ansa...

Abstract Atrial natriuretic peptide (ANP) was measured in plasma of dogs and rabbits by radioimmunoassay (RIA) using a commercially available anti α-ANP serum compared to our measurements ANP rats humans. Plasma concentration dog coronary sinus (234.9 ± 41.0 pg/ml) significantly greater than systemic arterial blood (81.2 8.4 pg/ml). Gel filtration resulted an peak with the elution volume (Ve) synthetic atriopeptin III (AIII) minor eluting void (Vo). Rabbit 53.3 4.3 pg/ml yielded one peak, Ve...

Infusions of norepinephrine led to a significant sevenfold increase in plasma immunoreactive atrial natriuretic peptide, while infusions acetylcholine caused no change the level peptide. Efferent stimulation right vagus nerve or inferior cervical ganglion anesthetized, vagotomized rabbits produced changes The findings suggest that mechanism by which releases peptide is not result direct action on cardiac myocytes.

To determine the effects of exercise during pregnancy on cardiovascular system their progeny, female Sprague-Dawley rats were assigned to one four groups: 1) trained-nontrained (T-NT) animals who ran for 7 wk at 35 m/min before but not pregnancy; 2) T-T and 32 3) control (C) did exercise; 4) NT-T only (16 m/min) pregnancy. Significant increases in skeletal muscle cytochrome found between exercised (NT-T, T-T) nonexercised (C, T-NT) mothers; C had significantly lower maximal oxygen...

Random urine Ca/creatinine (UCa/Cr) is used to estimate 24-hour Ca excretion. However, due decreased creatinine excretion in children with muscle mass (DMM), UCa/Cr overestimates their excretion.To evaluate whether DMM random Ca/osmolality (UCa/Osm) can accurately predict hypercalciuria (24-hour > 4.0 mg/kg) and at which "cutoff" value.19 29 normal (NMM), ages 6 - 17 years, were studied. was diagnosed based on clinical findings serum creatinine, confirmed by low Over 24 hours, subjects...

Distension of the left atrium in chloralose anaesthetized dogs causes a diuresis and dilution urine. It has been reported previously that if distension is maintained then urine flow reaches peak after 50 min declines. A radioimmunoassay was used to measure plasma arginine vasopressin (AVP) at 10-min intervals before, during, atrial for 90 min. Plasma AVP decreased during did not increase until removed.Urine volume free-water clearance increased osmolality decreased, reach maximum changes...

Immunoreactive atrial natriuretic peptide (iANP) levels in plasma of edematous rats with an experimental nephrotic syndrome produced by the injection puromycin aminonucleoside (PAN) were not different from those untreated rats. To test ability to secrete iANP response a volume stress, subjected 20% expansion their estimated blood volumes using donor PAN-treated had very small and diuretic responses compared rats; however, there was no difference secretory iANP, which increased approximately...

The injection or infusion of NIAMDD prolactin (NIH P-S-10) into unanesthetized rats resulted in water and electrolyte retention with a large increase urine osmolality but no effect on glomerular filtration rate. Since these effects output were also observed homozygous Brattleboro rats, the antidiuretic activity could not have caused by release endogenous hormone. Radioimmunoassay NIH showed that it was contaminated vasopressin (20 ng/mg prolactin). By comparison, Sigma had excretion...

The objective of this study was to determine if water temperature influenced exercise-induced hyperthermia in swim-trained pregnant rats and the resulting fetal development. Pregnant Sprague-Dawley with 6 weeks pre-pregnancy training were exercised daily from day 1 18 gestation that 34.6±0.4 °C (Cool Water Swimmers - CWS) or 37.6±0.1 (Warm WWS), for one hour/day. During time period another group immersed neck warm (37.6±0.2 °C) Controls WWC). On 19 all animals sacrificed development...

The elimination from plasma of the peptidec hormones vasopressin (VP) and atrial natriuretic peptide (ANP) as well time course release these after a physiological stimulus were studied in anesthetized rabbits. As an inverse relationship was found to exist between carotid sinus pressure IR-ANP, decrease 60 mm Hg used stimulate ANP VP release. iv injection involved rapid initial phase slow late component, with corresponding half-life (tvO values 0.9 5.4 min, respectively. After reduction Hg,...

Plasma vasopressin (AVP) concentration in dogs anaesthetized with chloralose was measured by radioimmunoassay and within the range of 2‐5 pg/ml. during control periods. Distension left atrium led 2 min to a fall plasma AVP which reached steady lower value 4 min. After cessation atrial distension returned pre‐distention values Cooling cervical vagosympathetic nerves 8‐10 degrees C rise concentration. Atrial cooling vagi resulted further increase

We have previously shown that testicular fluid contains factors can inhibit luteinizing hormone (LH)-stimulated androgen production by Leydig cells, and others reported the presence of immunoreactive vasopressin (iAVP) in testes as well vitro inhibition cell-androgen production. In current report, we used an established radioimmunoassay (RIA) to measure concentration iAVP related changes disruption seminiferous tubules. Spermatogenesis was disrupted adult rats surgically establishing...

The influence of aortic baroreceptors and vagal afferent nerves on the release immunoreactive vasopressin (iVP) atrial natriuretic factor (iANF) was examined in anaesthetized rabbits. Changes plasma concentrations iVP iANF, heart rate, mean arterial pressure, right pressure were measured response to blood volume changes (+20, +10, −10, −20%). Carotid sinus maintained at 100 mmHg (1 = 133.3 Pa), performed before after bilateral vagotomy (VNX) all experiments. Two experimental groups studied:...

Morphine was administered intravenously to normal and Brattleboro (genetically deficient in antidiuretic hormone) rats. The injection caused a significant decrease mean arterial pressure urine flow all animals, including those homozygous for diabetes insipidus. It is suggested that the effect of morphine may occur independently ADH release.

The time course of changes in the plasma concentration immunoreactive atrial natriuretic peptide (iANP) accompanying tachycardia was measured anesthetized rabbits. In contrast to hemodynamic changes, which occurred within 1st min tachycardia, iANP increased gradually and did not reach significantly elevated levels until 10 into stimulation period. After 20 almost 200 pg/ml. Immunoreactive ANP prior following extraction. Although basal were higher unextracted than extracted (62 vs. 22 pg/ml),...