A5074870866- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV, TB, and STIs Epidemiology
- HIV/AIDS Research and Interventions
- Bacteriophages and microbial interactions
- HIV, Drug Use, Sexual Risk
- Hepatitis C virus research
- Pneumocystis jirovecii pneumonia detection and treatment
- Virus-based gene therapy research
- Syphilis Diagnosis and Treatment
- Animal Disease Management and Epidemiology
- RNA Interference and Gene Delivery
- Sex work and related issues
- Cytomegalovirus and herpesvirus research
- Genomics and Phylogenetic Studies
- Herpesvirus Infections and Treatments
Central Research Institute of Epidemiology
2014-2024
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2017-2022
Central Research Institute
2022
Background Eastern Europe and Central Asia (EECA) is one of the regions where HIV epidemic continues to grow at a concerning rate. Antiretroviral therapy (ART) coverage in EECA countries has significantly increased during last decade, which can lead an increase risk emergence, transmission, spread variants with drug resistance (DR) that cannot be controlled. Because genotyping performed these countries, data about DR are limited or unavailable. Objectives To monitor circulating HIV-1 genetic...
Aim. To simultaneously analyze HIV-1 samples from all Russian regions to characterize the epidemiology of HIV infection in country as a whole. Subjects and methods. The most extensive study was conducted examine nucleotide sequences pol gene isolated HIV-positive persons different Russia, with diagnosis date being fixed during 1987—2015. genome were analyzed using computer programs on-line applications identify virus subtype new recombinant forms. Results. 1697 findings that genetic variant...
In Russia, antiretroviral therapy (ART) coverage has significantly increased, which, in the absence of routine genotyping testing, could lead to an increase HIV drug resistance (DR). The aim this study was investigate patterns and temporal trends DR as well prevalence genetic variants treatment-naïve patients from 2006 2022, using data Russian database (4481 protease reverse transcriptase 844 integrase gene sequences). variants, mutations (DRMs) were determined Stanford Database. analysis...
Tat protein is a major factor of HIV (human immunodeficiency virus) transcription regulation and has other activities. characterized by high variability, with some amino acid substitutions, including subtypespecific ones, being able to influence on its functionality. type 1 (HIV-1) sub-subtype A6 the most widespread in Russia. Previous studies polymorphisms structural regions variant have shown numerous characteristic features; however, polymorphism not been studied.Goals tasks. The main...
Although HIV-1 subtype A has predominated in Russia since the end of 20th century, other viral variants also circulate this country. The dramatic outbreak G 1988-1990 represents origin variant spreading Russia. However, full genome sequencing nosocomial and an analysis current circulating have not been conducted. We performed near full-length phylogenetic recombination analyses 11 samples; samples were determined to be based on pol region. Three reliably obtained from patients infected...
Aim: to analyze the prevalence, structure of drug resistance and mutations in protease reverse transcriptase genes HIV-1 among treatment naïve patients. Materials methods. We analyzed sequences from 1560 HIV-infected patients all Federal Districts Russian Federation with first positive immune blot during 1998–2017. Sequences were for presence predicted antiretroviral drugs using two algorithms — Stanford HIVDR Database (HIVdb) 2009 SDRM list (CPR). Results. The prevalence was 11,1%. More...
The development of sequencing technologies and bioinformatic analysis made it possible to conduct molecular epidemiological studies, in which nucleotide sequences the human immunodeficiency virus (HIV) are used as information added patient profile. From a practical perspective, studies prevalence HIV drug resistance (HIVDR) highest significance. To promote such different countries use databases that serve repositories genetic information. Russian HIVDR database (https://hivresist.ru/) was...
Objective. To develop and validate the method of dual HIV infection detection based on results Sanger sequencing to evaluate frequency in Russia. Materials methods. We carried out HIV-1 pol gene fragment model samples infection, calculation degeneracy bases index (DB-index), synonymity (SM-index) determination their threshold values for detection. analyzed nucleotide sequences (n = 1561) taken from Russian patients. Results. The 34 DB-index 0.05 SM-index allow detecting effectively using...
The increased antiretroviral therapy (ART) coverage of patients in the absence routine genotyping tests and context active labor migration highlight importance HIV-1 drug resistance (DR) surveillance Armenia. We conducted a two-phase pretreatment DR (PDR) study 2017-2018 (phase I; 120 patients) 2020-2021 II; 133 according to WHO-approved protocol. analysis genetic variants showed high degrees viral diversity, with predominance A6. prevalence any PDR was 9.2% phase I 7.5% II. protease...
The human immunodeficiency virus (HIV) Nef protein is one of the key factors determining infectivity and replicative properties HIV. With ability to interact with numerous proteins host cell, this provides maximum level production protects it from immune system. main activities are associated a decrease in expression CD4 receptor major histocompatibility complex class I molecules (MHC-I), as well rearrangement cytoskeleton. These determined by structure several motifs nef gene encoding it,...
Background. The increased antiretroviral therapy (ART) coverage of patients in the absence routine drug resistance (DR) tests highlight importance HIV-1 surveillance Armenia. Aim. aim this study was a determination prevalence DR on large-scale cohort HIV-infected citizens Republic Armenia who had no experience taking drugs. Materials and methods. carried out more than 20% PLHIV resulting 982 nucleotide sequences pol gene fragment, encoding protease reverse transcriptase region, as well 367...
Most HIV-1 tropism studies have involved non-A subtypes. Our aim was to study the prevalence of R5- and non-R5-tropic variants occurrence relative CD4 counts, treatment experiences, transmission routes other features infection in Russia, where subtype A is presumably predominant. In this multicenter, single-step, cross-sectional, epidemiologic study, 943 HIV-1-infected patients were enrolled at 12 AIDS centers throughout Russia. Viral determined using a genotype method-based kit. The V3 loop...
The new standing order of Russian Ministry Health for the use assisted reproductive technologies (ART) (in force since 2013) permits ART discordant couples with one partner infected HIV. This permission puts on clinics an additional responsibility due to risks HIV transmission. With aim decrease possibility nosocomial infection mother and child, method detection in washed sperm was developed validated.
Aim: to carry out the comparative analysis of Russian kits for HIV-1 viral load (VL) measuring: AmpliSens HIV-monitor-FRT, HIV-monitor-Duo-FL, RealBest RNA HIV quantitative, HIV-GEN quantitative. Materials and methods : we analyzed collection clinical blood plasma samples with VL 590–2,05×106 copies/ml, dilutions 2nd International Standard, as well panels subtypes recombinant forms group M. The convergence results was determined using Spearman correlation Bland-Altman method. Results. A high...
Introduction Maraviroc inhibits CCR5-tropic HIV-1 across different subtypes in vitro and has demonstrated efficacy clinical trials. V3-loop amino acid variants observed individual maraviroc-resistant viruses have not been found to be predictive of reduced susceptibility. Sequence-database searches that approximately 7.3% naturally encode these variants, raising concerns regarding potential pre-existing resistance. A study from Russia reported combinations same acids are present the V3 loops...
Objective. Accurate identification of recent HIV-1 infection cases will ensure a more effective and precise assessment the dynamics virus transmission, time between diagnosis, quality screening prevention programs. This study was undertaken to adjust testing algorithm using cohort patients, in whom since known. Materials methods. We used blood plasma samples obtained from 264 HIV-infected patients with known date infection. All were analyzed two serological assays aimed differentiate...