A5087288466- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- RNA modifications and cancer
- Click Chemistry and Applications
- RNA Research and Splicing
- Chemical Synthesis and Analysis
- RNA regulation and disease
- DNA and Nucleic Acid Chemistry
- Lymphatic System and Diseases
ETH Zurich
2012-2018
École Polytechnique Fédérale de Lausanne
2012
A chemical method for the post-synthetic labeling of pre-miRNAs on solid support using easily accessible reagents was developed. The procedure employed to generate a library 31 pre-microRNAs carrying labels commonly used in biology, including Cy3, trioxalen, biotin, and BHQ-1. As service our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may be re-organized online delivery, but not copy-edited or typeset. Technical issues...
MicroRNAs (miRNAs) originate from stem-loop-containing precursors (pre-miRNAs, pri-miRNAs) and mature by means of the Drosha Dicer endonucleases their associated factors. The let-7 miRNAs have prominent roles in developmental differentiation regulating cell proliferation. In cancer, tumor suppressor function is abrogated overexpression Lin28, one several RNA-binding proteins that regulate biogenesis interacting with conserved motifs close to cleavage site. Using vitro assays, we identified a...
We synthesized a miR-122 antimiR library in which drug-like fragments were site-specifically introduced to short 2'-O-methyl-RNAs. At some sites selected elevated cellular activity that of an unmodified 23mer antimiR, whereas at others the same abolished activity. The potency antimiRs correlated with uptake into miRISC.
Eine chemische Methode für die nachsynthetische Markierung von Prä-MikroRNAs (Prä-miRNAs) auf festem Träger mit einfach zugänglichen Reagentien wird beschrieben. Die wurde genutzt, um eine Bibliothek 31 gebräuchlichen Markierungen, einschließlich Cy3, Trioxalen, Biotin und BHQ-1, herzustellen. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed may be re-organized for online delivery, but not...
We report, herein, a new class of RNAi trigger molecules based on the unconventional parallel hybridization two oligonucleotide chains. have prepared and studied several stranded (ps) duplexes, in which orientation is achieved through incorporation isoguanine isocytosine to form reverse Watson-Crick base pairs ps-DNA:DNA, ps-DNA:RNA, ps-(DNA-2′F-ANA):RNA, ps-DNA:2′F-RNA duplexes. The formation these duplexes was confirmed by UV melting experiments, FRET CD studies. In addition, NMR...