A5065065276- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Supramolecular Self-Assembly in Materials
- Prion Diseases and Protein Misfolding
- Glycosylation and Glycoproteins Research
- Aortic Disease and Treatment Approaches
- Advanced Fluorescence Microscopy Techniques
- Botulinum Toxin and Related Neurological Disorders
- Enzyme Structure and Function
- Bone Tissue Engineering Materials
- Neurological diseases and metabolism
- Aortic aneurysm repair treatments
- Cardiac Health and Mental Health
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Signaling Pathways in Disease
- Force Microscopy Techniques and Applications
- Ubiquitin and proteasome pathways
- Protein Structure and Dynamics
- Peptidase Inhibition and Analysis
- Quantum Dots Synthesis And Properties
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Electron Microscopy Techniques and Applications
University of British Columbia
2025
University of the Fraser Valley
2024
Nitte University
2022
University Health Network
2022
Erasmus MC
2018-2021
Erasmus MC Cancer Institute
2021
Oncode Institute
2021
University of Twente
2014-2018
National Institute of Immunology
2011
Thioflavin-T (ThT) is the most commonly used fluorescent dye for following amyloid formation semi-quantitatively in vitro, specifically probing fibrillar cross-β-sheet content. In recent years, structural polymorphism of fibrils has been shown to be an important aspect formation, both vitro and neurodegenerative diseases. Therefore, understanding ThT-amyloid interactions context amyloids necessary correct interpretation ThT fluorescence data. Here we study influence fibril morphology on...
Amyloid polymorphs have become one of the focal points molecular studies neurodegenerative diseases like Parkinson's disease. Due to their distinct biochemical properties and prion-like characteristics, insights into origin stability amyloid over time are crucial for understanding potential role polymorphism in these diseases. Here, we systematically study fibrillization recombinantly produced human α-synuclein (αSyn) an extended period unravel temporal evolution polymorphism. We follow...
Abstract The formation of α-synuclein (α-S) amyloid aggregates, called Lewy bodies (LBs), is a hallmark Parkinson’s disease (PD). function LBs in the process however still unclear; they have been associated with both neuroprotection and toxicity. To obtain insight into this contradiction, we induced α-S inclusions, using three different induction methods SH-SY5Y cells rat-derived primary neuronal cells. Using confocal STED microscopy observed induction-dependent differences inclusion...
Under aggregation-prone conditions, soluble amyloidogenic protein monomers can self-assemble into fibrils or they fibrillize on preformed fibrillar seeds (seeded aggregation). Seeded aggregations are known to propagate the morphology of in event cross-seeding. However, not all proteins cross-seed aggregation. Cross-seeding has been proposed be restricted either because differences sequences conformations between and monomers. Here, we examine cross-seeding efficiency three α-synuclein...
Breast cancer type two susceptibility protein (BRCA2) is an essential in genome maintenance, homologous recombination (HR), and replication fork protection. Its function includes multiple interaction partners requires timely localization to relevant sites the nucleus. We investigated importance of highly conserved DNA-binding domain (DBD) C-terminal (CTD) BRCA2. generated BRCA2 variants missing one or both domains mouse embryonic stem (ES) cells defined their contribution HR dynamic nucleus,...
A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This facilitates rapid room-temperature digestions with multienzyme capabilities. Three methodologies were tested: within microcentrifuge tubes,
BRCA2 is a key breast cancer associated protein that predicted to have interspersed regions of intrinsic disorder. Intrinsic disorder coupled with large size likely allows sample broad range conformational space. We expect the resulting dynamic arrangements domains are functionally important aspect its role in homologous recombination DNA repair. To determine architectural organization and landscape BRCA2, we used scanning force microscopy based single molecule analyses map flexible...
Abstract BRCA2 is an essential protein in genome maintenance, homologous recombination and replication fork protection. Its function includes multiple interaction partners requires timely localization to relevant sites the nucleus. We investigated importance of highly conserved DNA binding domain (DBD) C-terminal (CTD) BRCA2. generated variants missing one or both domains mouse ES cells defined their contribution HR dynamic nucleus, by single particle tracking mobility. Changes molecular...