A5011272523- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Genomics and Chromatin Dynamics
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Legume Nitrogen Fixing Symbiosis
University of Lausanne
2020-2022
Max Planck Institute of Biochemistry
2016
Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood. Here, we investigate the role of Smc ATPase activity recruitment to Bacillus subtilis chromosome. We demonstrate that targeting ParB/parS loading sites is strictly dependent on engagement head domains relies an open organization coiled coils. find dimerization hinge domain stabilizes closed rods hinders as well...
Three-component ParABS systems are widely distributed factors for plasmid partitioning and chromosome segregation in bacteria. ParB acts as adaptor protein between the 16–base pair centromeric parS DNA sequences proteins ParA Smc (structural maintenance of chromosomes). Upon cytidine triphosphate (CTP) binding, dimers form clamps that spread onto -flanking by sliding, thus assembling so-called partition complex. We show here CTP hydrolysis is essential efficient but largely dispensable...
SMC complexes are widely conserved ATP-powered DNA-loop-extrusion motors indispensable for organizing and faithfully segregating chromosomes. How translocate along DNA loop extrusion what happens when two meet on the same molecule is largely unknown. Revealing origins consequences of encounters crucial understanding folding process not only bacterial, but also eukaryotic Here, we uncover several factors that influence bacterial chromosome organization by modulating probability such clashes....
Chromosomes readily unlink and segregate to daughter cells during cell division, highlighting a remarkable ability of organize long DNA molecules. SMC complexes promote organization by loop extrusion. In most bacteria, chromosome folding initiates at dedicated start sites marked the ParB/parS partition complexes. Whether recognize specific structure in complex or protein component is unclear. By replacing genes Bacillus subtilis with orthologous sequences from Streptococcus pneumoniae, we...
SMC complexes, loaded at ParB-
Abstract SMC complexes are widely conserved ATP-powered loop extrusion motors indispensable for the faithful segregation of chromosomes during cell division. How translocate along DNA and what happens when two meet on same molecule is largely unknown. Revealing origins consequences encounters crucial understanding folding process not only bacterial, but also eukaryotic chromosomes. Here, we uncover several factors that influence bacterial chromosome organization by modulating probability...
Abstract Three-component ParABS systems are widely distributed factors for plasmid partitioning and chromosome segregation in bacteria. ParB protein acts as an adaptor between the 16 bp centromeric parS DNA sequences ATPase ParA. It accumulates at high concentrations near a site by assembling partition complex. dimers form sliding clamp whose closure requires CTP binding. The mechanism underlying loading role of hydrolysis however remain unclear. We show that is dispensable Smc recruitment...
Abstract Chromosomes readily unlink from one another and segregate to daughter cells during cell division highlighting a remarkable ability of organize long DNA molecules. SMC complexes mediate chromosome folding by loop extrusion. In most bacteria, start extrusion at the ParB/ parS partition complex formed near replication origin. Whether they are recruited recognizing specific structure in or protein component is unknown. By replacing genes Bacillus subtilis with orthologous sequences...
Abstract SMC complexes, loaded at ParB- parS sites, are key mediators of chromosome organization in bacteria. ParA/Soj proteins interact with ParB/Spo0J a pathway involving ATP-dependent dimerization and DNA binding, leading to segregation loading. In Bacillus subtilis , also regulates replication initiation, along is involved cell cycle changes during endospore formation. The first morphological stage sporulation the formation an elongated structure called axial filament. We now show that...