Vitrification of oocytes has been shown to be closely associated with increased levels reactive oxygen species (ROS) and apoptotic events. However, little information is available the effect melatonin on ROS events in vitrified oocytes. Therefore, we studied bovine by supplementing vitrification solution or vitro maturation (IVM) 10(-9) m melatonin. We analyzed ROS, mitochondrial Ca(2+) (mCa(2+) ) membrane potential (ΔΨm), externalization phosphatidylserine (PS), caspase-3 activation, DNA...

Cryodamage is a major problem in semen cryopreservation, causing changes the levels of proteins that influence function and motility spermatozoa. In this study, protein samples prepared from fresh frozen-thawed boar spermatozoa were compared using isobaric tags for relative absolute quantification (iTRAQ) labeling technique coupled to 2D LC-MS/MS analysis. A total 41 differentially expressed identified quantified, including 35 present at higher six lower by least mean 1.79-fold (P<0.05). On...

The zootechnical performances of broilers at the end rearing period depend in part on quality day-old chicks placement. is highly affected by incubation conditions, hatch time (which determines spent hatcher under high temperature and humidity), handling after hatch. This article first presents an overview most relevant pre-incubation factors that affect chick quality: egg size, weight, eggs, sex embryos, age breeders, conditions duration storage. It then reviews important time, hatchability...

This study was designed to examine the recovery of mitochondrial function and endogenous antioxidant systems in vitrified oocytes during extended incubations. After 16 hr vitro maturation, bovine meiosis-II were vitrified, then surviving cultured an additional 8 hr. ATP content, synthase activity, expression F0 subunit 6 (ATP6) (ATP8) genes, reactive oxygen species (ROS) levels investigated this period (4 or hr). The results showed that: (1) content activities at post-warming (754.6 fmol,...

This study was conducted to evaluate the effect of a 12-h light, dark (12L : 12D) photoperiod green light during day 1 18 incubation time, on embryo growth, hormone concentration and hatch process. In test group, monochromatic provided by total 204 light-emitting diodes (522 nm) mounted in frame which placed above top tray eggs give even spread illumination. No light–dark cycle used control group. Four batches (n=300/group per batch) from fertile Ross 308 broiler breeders were this...

Abstract It has been suspected for many years that cattle possess two functional IgH gene loci, located on Bos taurus autosome (BTA) 21 and BTA11, respectively. In this study, based fluorescence in situ hybridization additional experiments, we showed all bovine genes were BTA21, only a truncated μCH2 exon was present BTA11. By sequencing of seven bacterial artificial chromosome clones screened from Hostein cow library, generated 678-kb continuous genomic sequence covering the IGHV, IGHD,...

This experiment studied the effect of transportation duration 1-d-old chicks on dehydration, mortality, production performance, and pododermatitis during growout period. Eggs from same breeder flock (Ross PM3) were collected at 35, 45, 56 wk age, for 3 successive identical experiments. In each experiment, newly hatched received 1 treatments hatchery before placement in on-site rearing facility: no corresponding to direct less than 5 min (T00), or 4 (T04) 10 h (T10) transportation. The housed...

Current methods of administering busulfan to remove the endogenous germ cells cause hematopoietic toxicity, require special instruments and a narrow transplantation time. We use direct testicular injection method for preparing recipients SSC transplantation. Male ICR mice (recipients) were divided into four groups, two experimental groups treated with bilateral 4 or 6 mg/kg/side (n = 60 per concentration group). Mice received an intraperitoneal (i.p.) 40 mg/kg 60, positive control)...

This study was designed to determine the effect of melatonin on in vitro maturation (IVM) and developmental potential bovine oocytes denuded cumulus oophorus (DOs). DOs were cultured alone (DOs) or with 10-9 M (DOs + MT), cumulus-oocyte complexes (COCs) without as control. After IVM, meiosis II (MII) rates DOs, reactive oxygen species (ROS) levels, apoptotic parthenogenetic blastocyst MII determined. The relative expression ATP synthase F0 Subunit 6 8 (ATP6 ATP8), bone morphogenetic protein...

We investigated the efficacy of a single bacterium strain, Bacillus subtilis (B. subtilis) YW1, on performance, morphology, cecal microbiota, and intestinal barrier function laying hens. A total 216 28-week-old Hy-line Brown hens were divided into three dietary treatment groups, with six replicates 12 birds each for 4 weeks. The control group (Ctr) was fed basal diet T1 T2, supplemented B. at dose rate 5 × 108 CFU/kg 2.5 109 CFU/kg, respectively. Dietary supplementation did not significantly...

The present study was designed to determine the effects of vitrification on promoter methylation and expression levels pluripotency differentiation genes in mouse blastocysts. Promoter region CpG patterns octamer-binding transcription factor (Oct4), Nanog homeobox (Nanog), caudal-type 2 (Cdx2), heart neural crest derivatives-expressed transcript 1 (Hand1) were analyzed fresh vitrified Methylation measured by bisulphate mutagenesis sequencing; gene determined real-time reverse...

SUMMARY Differential expression of genes leads to variations in the phenotypes X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes bovine sperm were identified by a combination suppression subtractive hybridization (SSH), cDNA microarray, sequence‐homology analysis. Microarray data Significance Analysis Microarrays software used identify 31 genes, only four which previously identified. These involved fundamental life processes...

Natural mating colony cages for layer breeders have become commonplace in China. However, feather pecking (FP) and cannibalism are prominent this system. The objective of study was to investigate the effects four light-emitting diode (LED) light colors (white: WL, red: RL, yellow-orange: YO, blue-green: BG) with two intensities each color, on FP, plumage condition, cannibalism, fear, stress. A total 32 identical were used eight treatments (four replicates treatment). For both intensities,...

Less evidence is available currently to reveal whether the immune system and productivity of laying hens change under long periods ammonia exposure in hot climate. The present study was conducted determine effects chronic high temperature concentrations on health, response, reproductive hormones commercial hens. A total five hundred seventy six 20-week-old (Hy-Line Brown) were used this study. Birds housed cages (4 birds per cage) received 16-wk treatments 6 artificial environmental...

In a cold climate, ensuring indoor air quality and heat preservation simultaneously has always been difficult problem in the poultry house. The current study was carried out order to determine effects of chronic low temperature ammonia concentration on productive performance egg commercial laying hens. 576 18-week-old Hy-line Brown hens were used this study. Birds housed cages received for 20-week exposure six artificial environmental chambers. randomly assigned into treatments: treatment 1...

Canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) cause severe disease in young animals, pups, kittens. CPV-2 evolved from FPV by altering the species-specific binding of viral capsid to host receptor, i.e., transferrin receptor (TfR), genetic variants have been identified specific VP2 amino acid residues (297, 426). Early studies focused on main protein VP2; however, there limited non-structural NS1. In this study, we clinical samples dogs cats northern China during...

This study investigated variations in eggshell temperature (T(egg)) during the hatching process of broiler eggs. Temperature sensors monitored embryo by registering T(egg) every minute. Measurements carried out on a sample 40 focal eggs revealed drops between 2 to 6°C last 3 d incubation. Video cameras recorded and served as gold standard reference for manually labeling hatch times chicks. Comparison time individuals synchronization with 99% correlation coefficient an absolute average...

Bovine viral diarrhea virus (BVDV) is one of the most important pathogens in cattle. Previously, BVDV sub-genotypes 1b, 1c, 1d, and 1 m were detected China. However, isolation type 1a from cattle has not been reported In 2010, twenty nasal swabs blood samples collected suspected infection Henan province, A isolate was isolated using cell culture, pathogenesis studied. Virus performed on MDBK cells. The identification conducted by RT-PCR, neutralization test immunofluorescence assay. order to...