A5079813601- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Climate Change and Health Impacts
- Disaster Response and Management
- COVID-19 and healthcare impacts
- Air Quality and Health Impacts
Stanford University
2020-2023
Broad Institute
2020
Center for Systems Biology
2018
Harvard University
2018
Abstract Recent outbreaks of viral hemorrhagic fevers (VHFs), including Ebola virus disease (EVD) and Lassa fever (LF), highlight the urgent need for sensitive, deployable tests to diagnose these devastating human diseases. Here we develop CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting (EBOV) (LASV), with both fluorescent lateral flow readouts. We demonstrate on laboratory clinical samples sensitivity assays capacity SHERLOCK platform handle virus-specific diagnostic challenges....
Abstract Lassa fever, a hemorrhagic fever caused by virus (LASV), is endemic in West Africa. It difficult to distinguish febrile illnesses that are common Africa from based solely on patient’s clinical presentation. The field performance of recombinant antigen-based immunoassays was compared quantitative polymerase chain assays (qPCRs) using samples subjects meeting the case definition presenting Kenema Government Hospital Sierra Leone. (ReLASV) enzyme-linked immunosorbant assay (ELISA) for...
Climate change is a public health emergency.1Solomon CG LaRocque RC change–a emergency.N Engl J Med. 2019; 380: 209-211Crossref PubMed Scopus (75) Google Scholar Clinicians worldwide now face the reality of caring for patients during wildfires, heat waves, floods, and shifting infectious disease patterns. Health-care workers need better preparation to care communities facing effects climate change.2Salas RN The crisis clinical practice.N 2020; 382: 589-591Crossref (22) Medical students have...
Abstract Viral hemorrhagic fevers (VHFs) remain some of the most devastating human diseases, and recent outbreaks Ebola virus disease (EVD) 1,2 Lassa fever (LF) 3,4 highlight urgent need for sensitive, field-deployable tests to diagnose them 5,6 . Here we develop CRISPR-Cas13a-based (SHERLOCK) diagnostics targeting (EBOV) (LASV), with both fluorescent lateral flow readouts. We demonstrate on laboratory clinical samples sensitivity these assays capacity SHERLOCK platform handle virus-specific...